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Recently,there is an interest in biomatrials and joint replacement.Therefore,the search for innovation of orthopeadic biomaterials is always reasonable.In this study, we investigated the osteointegration of human osteoblast-like cells( MG 63) in cultures on the following surface-modified metallic and polymeric materials, developed for construction of artificial hip joint replacements:Ti-6Al-4V alloy modified by plasma-spraying,polishing,plasma-spraying and coating with amorphous hydrogenated carbon(a-C:H)or polishing and coating with a-C:H;stainless steel AISI 316 LWM modified with plasma-spraying or polishing;non-modified or polished polyetheretherketon (PEEK) Optima,and non-modified or polished ultra-high molecular weight polyethylene (UHMWPE).Standard cell culture polystyrene dishes (TPP, Switzerland)served as a reference material.On day 1 after seeding, the cell adhesion was in lower number on all materials than on control polystyrene dishes,except Ti-6Al-4V treated by plasma-spraying and coating with a-C:H, where the cell number, as well as the size of the cell adhesion area,were comparable with the values obtained on the reference material. As revealed by (ELISA) the cells on plasma-sprayed and a-C:H-coated Ti-6Al-4V contained the highest concentration of integrin (by 132% compared to the value on polystyrene)fibronectin and vitronectin.On day 3 after seeding, also the cell number on Ti-6Al-4V treated by polishing and coating with a-C:H equaled with the value on the control polystyrene.A relatively high cell number was also obtained on polished stainless steel AISI 316 LWM.The cells on these samples contained the highest concentrations of integrins alpha v vinculin and osteocalcin.On day 8 after seeding,the cell numbers on almost all samples equaled with the values on control polystyrene,except the numbers on polished and relatively highly hydrophobic UHMWPE.On plasma-sprayed and a-C:H-coated Ti-6Al-4V,and also non-modified PEEK,the cell numbers even exceeded the values on polystyrene dishes.In addition, the cells on non-modified PEEK contained one of the highest concentrations of talin,and on polished PEEK,the highest concentration of osteopontin.Therefore, we conclude that plasma-sprayed and a-C:H-coated Ti-6Al-4V, polished stainless steel AISI 316 LWM and PEEK seem to be suitable materials for constructing bone-anchoring parts of joint prostheses,while polished UHMWPE can be used for creation of articular surfaces,where the cell adhesion and growth is not desirable. |
