Preparation of a highly specific polyclonal antibody against and its use in development of ELISA

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The prokaryotic expression, polyclonal antibody preparation, and subcellular localization of the transmembrane protein NS2A of the duck Tembusu virus

 

Duck Tembusu virus (DTMUV) is a single-stranded, positive-sense RNA arbovirus, belonging to the genus Flavivirus, household Flaviviridae. As a transmembrane protein, non-structural protein 2A (NS2A) performs an vital position in virion meeting, replication advanced and antagonizing host immune response. Since NS2A protein comprises many hydrophobic amino acids, it’s arduous to realize the full-length protein of NS2A for prokaryotic expression. Subsequently, to make a deep examine, prokaryotic expression and polyclonal antibody preparation of truncated DTMUV NS2A was carried out.

The truncated NS2A gene (178-450 bp) was obtained, and sub-cloned into the prokaryotic vector pGEX-4T-1 (pGEX-4T-1-NS2A178-450bp). Subsequently, the recombinant GST-NS2A60-150aa protein was efficiently expressed in E. coli BL21 (DE3) with the induction by 0.Three mmol/l isopropyl β-D-thiogalactoside (IPTG) for six h at 37°C. The GST-NS2A60-150aa protein was extracted from the gel. The BALB/c mice have been immunized with the purified recombinant NS2A protein to organize polyclonal antibodies in opposition to the truncated NS2A protein.

The titer of the polyclonal antibodies, decided by enzyme-linked immunosorbent assay (ELISA) evaluation, was 1:128 000. The specificity of the polyclonal antibodies (mPAb-DTMUV-NS2A60-150aa) have been verified by Western blot evaluation. Moreover, the oblique immunofluorescence (IFA) was carried out to discover the subcellular localization of NS2A. NS2A protein was, within the transfected cells, positioned primarily round nucleus within the endoplasmatic reticulum.

Taken collectively, our examine supplied a helpful software for the additional exploration of the organic features and molecular mechanism of DTMUV NS2A. Key phrases: duck Tembusu virus; non-structural protein 2A; prokaryotic expression; polyclonal antibodies; subcellular location.

Prokaryotic expression of mouse Stra8 and preparation and identification of rabbit anti-mouse Stra8 polyclonal antibody

 

Goal To prokaryotically categorical mouse stimulated by retinoic acid gene 8 (Stra8) and put together rabbit anti-mouse Stra8 polyclonal antibody. Strategies The recombinant plasmid pET28a-Stra8 was constructed by cloning expertise and recognized by double enzyme digestion and sequencing, after which reworked into E. coli BL21 (DE3). The expression of Stra8 recombinant protein was induced by IPTG. The prokaryotic protein was purified by His-TAG affinity chromatograph after which used to immune New Zealand white rabbits to acquire Stra8 polyclonal antibody. ELISA,

Western blot and immunofluorescence assays have been used to find out the antibody titer, validity and specificity, respectively. Outcomes The recombinant plasmid pET28a-Stra8 was constructed efficiently as double enzyme digestion and sequencing confirmed, and the prokaryotic protein was expressed and purified successfully. The titer of the polyclonal antibody reached 1:106. Western blotting confirmed that the polyclonal antibody may particularly acknowledge native Stra8 protein within the testis.

Immunofluorescence assay revealed that the polyclonal antibody had good reactivity, and will acknowledge Stra8 protein in mouse testis. Conclusion Stra8 prokaryotic protein might be successfully induced in E. coli and particular rabbit anti-Stra8 polyclonal antibody has been ready.

Preparation of a extremely particular polyclonal antibody in opposition to and its use in growth of ELISA for willpower of in plasma

 

For therapeutic monitoring and pharmacokinetic research of the potent hypocholesterolaemic agent fluvastatin (FLV), a particular antibody was required for the event of a delicate enzyme-linked immunosorbent assay (ELISA) for the correct willpower of FLV in plasma. On this examine, a extremely particular polyclonal antibody in opposition to FLV has been ready. FLV was coupled to keyhole limpet hemocyanin (KLH) and bovine serum albumin (BSA) utilizing carbodiimide reagent. FLV-KLH conjugate was used as an immunogen. Feminine 8-weeks previous New Zealand white rabbits have been immunized with an emulsion of FLV-KLH with Freund’s adjuvant.

The immune response of the rabbits was monitored by direct ELISA utilizing FLV-BSA immobilized onto microwell plates as a strong section. The rabbit that confirmed the best antibody titer and affinity to FLV was scarified and its sera have been collected. The IgG fraction was remoted and purified by affinity chromatography on a protein A column.

The specificity of the purified antibody for FLV was evaluated by oblique aggressive ELISA utilizing numerous rivals from the FLV-structural analogues and therapeutic brokers used with FLV in a mix remedy. The excessive affinity of the antibody (IC50 = 150 pg ml-1) enabled the willpower of FLV in plasma at concentrations as little as 20 pg ml-1.

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Cixiophiopogon A

HY-N2175 5mg
EUR 705

Saikosaponin F

HY-N2178 1mg
EUR 243

Hypaphorine

HY-N2179 10mg
EUR 705

Pinoresinol dimethyl ether

HY-N2180 1mg
EUR 108

Acetylshikonin

HY-N2181 10mg
EUR 243

Episyringaresinol 4'-O-β-D-glncopyranoside

HY-N2182 10mg
EUR 838

Baimaside

HY-N2183 10mg
EUR 320

(5R,6E)-5-Hydroxy-1,7-diphenyl-6-hepten-3-one

HY-N2185 10mg
EUR 574

Deoxyshikonin

HY-N2187 5mg
EUR 342

Hederagenin 28-O-beta-D-glucopyranosyl ester

HY-N2190 5mg
EUR 1034

Ecliptasaponin D

HY-N2191 10mg
EUR 359

Hirsutine

HY-N2193 5mg
EUR 1362

Bergamottin

HY-N2194 25mg
EUR 497

Hirsuteine

HY-N2197 1mg
EUR 443

Podocarpusflavone A

HY-N2198 10mg
EUR 436

Sotetsuflavone

HY-N2199 5mg
EUR 807

Pseudoginsenoside RT1

HY-N2201 1mg
EUR 204

Vitexin2''-​O-​p-​coumarate

HY-N2203 5mg
EUR 1034

Esculentoside H

HY-N2205 10mg
EUR 640

rel-(8R,8'R)-Dimethyl-(7S,7'R)-bis(3,4-methylenedioxyphenyl)tetrahydro-furan

HY-N2207 5mg
EUR 1034

4-Hydroxylonchocarpin

HY-N2208 10mg
EUR 640

Angeloylgomisin H

HY-N2209 1mg
EUR 153

5,7,4'-Trihydroxy-8-Methylflavanone

HY-N2210 5mg
EUR 1034

Picfeltarraenin IB

HY-N2211 5mg
EUR 142

7-O-Methylaloeresin A

HY-N2214 1mg
EUR 379

Aloeresin D

HY-N2215 5mg
EUR 574

Rotundic acid

HY-N2217 10mg
EUR 705

Jionoside B1

HY-N2218 5mg
EUR 509

6-Formyl-isoophiopogonanone A

HY-N2220 5mg
EUR 838

Guaijaverin

HY-N2224 5mg
EUR 320

(-)-Epigallocatechin-3-(3''-O-methyl) gallate

HY-N2228 1mg
EUR 597

Rhapontigenin

HY-N2229 5mg
EUR 186

N-p-trans-Coumaroyltyramine

HY-N2230 1mg
EUR 179

Ganolactone B

HY-N2234 5mg
EUR 1034

Piceatannol 3'-O-glucoside

HY-N2237 5mg
EUR 257

Dipsanoside A

HY-N2238 1mg
EUR 400

6-Methoxykaempferol 3-O-Rutinoside

HY-N2239 1mg
EUR 487

Eupalinolide K

HY-N2240 5mg
EUR 1231

Hosenkoside F

HY-N2241 5mg
EUR 452

Hosenkoside G

HY-N2242 1mg
EUR 313

Hosenkoside K

HY-N2243 5mg
EUR 268

Hosenkoside M

HY-N2244 5mg
EUR 452

Gomisin H

HY-N2246 5mg
EUR 705

Hosenkoside A

HY-N2249 5mg
EUR 268

Hosenkoside B

HY-N2250 5mg
EUR 326

Crebanine

HY-N2255 20mg
EUR 443

(-​)​-​Salutaridine

HY-N2256 20mg
EUR 379

Curcumenol

HY-N2259 25mg
EUR 383

(-)-Cephaeline (dihydrochloride)

HY-N2260 5mg
EUR 340

Ipecoside

HY-N2261 1mg
EUR 179

8-Geranyloxypsoralen

HY-N2262 5mg
EUR 512

Skimmin

HY-N2263 10mg
EUR 337

Gymnemagenin

HY-N2268 5mg
EUR 705

Angeloylgomisin O

HY-N2271 5mg
EUR 838

23-Hydroxylongispinogenin

HY-N2274 1mg
EUR 313

21-​O-​Tigloylgymnemagenin

HY-N2275 5mg
EUR 1034

Kurarinone

HY-N2279 10mg
EUR 443

Deltonin

HY-N2283 5mg
EUR 486

Sophoflavescenol

HY-N2284 5mg
EUR 960

Fabiatrin

HY-N2285 10mg
EUR 705

2''-O-Rhamnosylicariside II

HY-N2289 5mg
EUR 337

Baohuoside VII

HY-N2290 5mg
EUR 1034

Rebaudioside G

HY-N2291 1mg
EUR 379

6"-O-Apiosyl-5-O-Methylvisammioside

HY-N2295 10mM/1mL
EUR 475

(2R,3R)-3,7-Dihydroxy-2-(4-hydroxyphenyl)-5-methoxy-8-(3-methylbut-2-en-1-yl)chroman-4-one

HY-N2296 5mg
EUR 1034

Camellianin A

HY-N2298 1mg
EUR 268

Kuwanon A

HY-N2300 25mg
EUR 1345

Pleuromutilin

HY-N2301 100mg
EUR 173

Fucoxanthin

HY-N2302 50mg
EUR 725

Aclacinomycin A hydrochloride

HY-N2306A 10mg
EUR 601

Kainic acid

HY-N2309 10mM/1mL
EUR 238

Ibotenic acid

HY-N2311 10mg
EUR 441

Mogrol

HY-N2312 1mg
EUR 187

Podocarpic acid

HY-N2318 10mg
EUR 119

Dihydroergocristine (mesylate)

HY-N2319 10mM/1mL
EUR 345

4'-Demethylpodophyllotoxin

HY-N2323 10mg
EUR 142

D-(+)-Cellobiose

HY-N2325 10mM/1mL
EUR 113

Oleamide

HY-N2327 10mg
EUR 153

Piperlongumine

HY-N2329 10mg
EUR 108

Methyllycaconitine citrate

HY-N2332A 50mg
EUR 612

Glycochenodeoxycholic acid

HY-N2334 10mg
EUR 119

Glycochenodeoxycholic acid (sodium salt)

HY-N2334A 10mM/1mL
EUR 113

Coumestrol

HY-N2335 10mg
EUR 715

Rosmarinic acid (racemate)

HY-N2336 100mg
EUR 173

11beta-Hydroxyprogesterone

HY-N2337 50mg
EUR 201

Cholesterol myristate

HY-N2338 250mg
EUR 133

Cholesteryl behenate

HY-N2339 10mg
EUR 147

D-(+)-Melezitose

HY-N2340 10mM/1mL
EUR 126

Palmitelaidic Acid

HY-N2341 10mg
EUR 119

Procyanidin C1

HY-N2342 10mg
EUR 681

polyclonal antibody in opposition to a recombinantly expressed Triticum aestivum RHT-D1A protein

 

Background: Lowered height-1 dwarfing alleles have an effect on DELLA proteins belonging to a household of putative transcriptional regulators that modulate plant development and growth. The Arabidopsis thaliana genome encodes 5 DELLA proteins, whereas monocot crops, reminiscent of rice, barley, and wheat, every have a single DELLA protein. In wheat, wild-type Rht-B1a and Rht-D1a genes encode DELLA proteins and have many alleles that comprise lesions.

 

Amongst them, Rht-B1b and Rht-D1b are the commonest mutant dwarfing alleles, which have performed a key half within the creation of high-yielding wheat varieties. Regardless of their basic roles in plant biology, till now, DELLA proteins in wheat have been primarily researched concerning the phenotypic impact of faulty Rht mutants on yield-related traits, with out research on the underlying mechanisms.

The RHT-1 protein has but to be detected in wheat tissues, owing to a scarcity of applicable molecular instruments for characterization of RHT perform and protein interactions in sign transduction. This examine is concentrated on the manufacturing of a polyclonal antibody to the wheat RHT-D1A protein.

 

Outcomes: To generate the anti-RHT-D1A antibody, we expressed and purified soluble 6xHis-tagged RHT-D1A. The purified recombinant RHT-D1A was injected into New Zealand white rabbits to generate polyclonal antiserum. The polyclonal anti-RHT-D1A antibody was purified by ammonium sulfate precipitation, adopted by affinity chromatography on protein A-agarose beads.

The purified polyclonal antibody was demonstrated to be efficient in immunoblotting, western blot hybridization, and immunoprecipitation. In wheat seedling extracts, the polyclonal antibody acknowledged a protein with a molecular mass near the expected molecular weight of the endogenous RHT-D1A protein. We additionally demonstrated that RHT-D1A disappears in response to exogenous and endogenous gibberellic acid.

 

Conclusion: The purified polyclonal antibody raised in opposition to the recombinant RHT-D1A protein is sufficiently particular and delicate and could possibly be a useful gizmo for future insights into upstream and downstream parts of DELLA-regulatory mechanisms in wheat crops.

 

Bacterial expression of 183-227aa area of HER3 extracellular area I and preparation and identification of its polyclonal antibodies

 

Goal: To arrange the recombinant peptide MVF-HER3 I composed of the 183-227aa peptide section of human epidermal development issue receptor 3 (HER3 I) and the measles virus protein 288-302 peptide section (MVF), and put together polyclonal antibodies (PcAb) in opposition to this recombinant peptide.

 

Strategies: The MVF-HER3 I gene was synthesized chemically and subcloned into pET21b or pET32a plasmid containing Thioredoxin (Trx) tag gene. The recombinant plasmids have been recognized by endonuclease digestion. MVF-HER3 I used to be expressed in E.coli BL21(DE3) cells underneath an optimum bacterial expression situation.

The fusion protein Trx-MVF-HER3 I used to be purified utilizing nickel ion affinity chromatography, and the purified protein was digested by enterokinase to take away Trx tag. The digested combination underwent additional nickel ion affinity chromatography to acquire purified MVF-HER3 I.

The purified MVF-HER3 I used to be used to immunize SD rats subcutaneously for getting ready anti-MVF-HER3 I PcAb. The titer of PcAb was decided utilizing ELISA. The bindings of anti-MVF-HER3 I PcAb to MVF-HER3 I, native HER3 and MCF7 cells have been analyzed utilizing immunoblotting, immunoprecipitation and laser confocal microscopy. The expansion inhibition impact of the antibodies on MCF7 cells cultured within the absence or presence of NRG was assessed utilizing sulforhodamine B.

 

Outcomes: The recombinant peptide gene couldn’t be expressed alone, however could possibly be effectively expressed after fusion with Trx gene underneath optimized situations. The fusion peptide MVF-HER3 I used to be efficiently ready from Trx-MVF-HER3 I. The anti-MVF-HER3 I PcAb, with a titer reaching 1: 512 000, particularly sure to MVF-HER3 I, acknowledged native HER3 and sure to the membrane of MCF7 cells. The obtained PcAb may dose-dependently inhibit the expansion of MCF7 cells no matter the presence or absence of NRG.

SF1 (Ab-82) Antibody

CSB-PA963117-100ul 100ul
EUR 316
Description: A polyclonal antibody against SF1 (Ab-82). Recognizes SF1 (Ab-82) from Human, Mouse. This antibody is Unconjugated. Tested in the following application: ELISA, WB, IHC, IF;WB:1:500-1:3000, IHC:1:50-1:100, IF:1:100-1:500

CCT7 Antibody

CSB-PA963179-
EUR 335
Description: A polyclonal antibody against CCT7. Recognizes CCT7 from Human. This antibody is Unconjugated. Tested in the following application: ELISA, WB;WB:1:500-1:3000

CCT7 Antibody

CSB-PA963179-100ul 100ul
EUR 316
Description: A polyclonal antibody against CCT7. Recognizes CCT7 from Human. This antibody is Unconjugated. Tested in the following application: ELISA, WB;WB:1:500-1:3000

Phospho-MAPT (Ser404) Antibody

CSB-PA963344-
EUR 335
Description: A polyclonal antibody against Phospho-MAPT (Ser404). Recognizes Phospho-MAPT (Ser404) from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, WB, IHC, IF;WB:1:500-1:1000, IHC:1:50-1:100, IF:1:100-1:200

Phospho-MAPT (Ser404) Antibody

CSB-PA963344-100ul 100ul
EUR 362
Description: A polyclonal antibody against Phospho-MAPT (Ser404). Recognizes Phospho-MAPT (Ser404) from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, WB, IHC, IF;WB:1:500-1:1000, IHC:1:50-1:100, IF:1:100-1:200

CST1 Antibody

CSB-PA963535-
EUR 335
Description: A polyclonal antibody against CST1. Recognizes CST1 from Human. This antibody is Unconjugated. Tested in the following application: ELISA, IHC, IF;IHC:1:50-1:100, IF:1:100-1:500

CST1 Antibody

CSB-PA963535-100ul 100ul
EUR 316
Description: A polyclonal antibody against CST1. Recognizes CST1 from Human. This antibody is Unconjugated. Tested in the following application: ELISA, IHC, IF;IHC:1:50-1:100, IF:1:100-1:500

Phospho-CCND1 (Ser90) Antibody

CSB-PA963558-
EUR 335
Description: A polyclonal antibody against Phospho-CCND1 (Ser90). Recognizes Phospho-CCND1 (Ser90) from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, WB;WB:1:500-1:1000

Phospho-CCND1 (Ser90) Antibody

CSB-PA963558-100ul 100ul
EUR 362
Description: A polyclonal antibody against Phospho-CCND1 (Ser90). Recognizes Phospho-CCND1 (Ser90) from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, WB;WB:1:500-1:1000

Histone H3K27me3 Antibody

CSB-PA963638-
EUR 335
Description: A polyclonal antibody against Histone H3K27me3. Recognizes Histone H3K27me3 from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, WB, IHC, IF, IP, ChIP;WB:1:500-1:2000, IHC:1:50-1:200, IF:1:50-1:200, IP:1:50-1:200, ChIP:1:50-1:200

Histone H3K27me3 Antibody

CSB-PA963638-100ul 100ul
EUR 380
Description: A polyclonal antibody against Histone H3K27me3. Recognizes Histone H3K27me3 from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, WB, IHC, IF, IP, ChIP;WB:1:500-1:2000, IHC:1:50-1:200, IF:1:50-1:200, IP:1:50-1:200, ChIP:1:50-1:200

MYOM1 Antibody

CSB-PA964006-
EUR 335
Description: A polyclonal antibody against MYOM1. Recognizes MYOM1 from Human. This antibody is Unconjugated. Tested in the following application: ELISA, IHC;IHC:1:50-1:100

MYOM1 Antibody

CSB-PA964006-100ul 100ul
EUR 316
Description: A polyclonal antibody against MYOM1. Recognizes MYOM1 from Human. This antibody is Unconjugated. Tested in the following application: ELISA, IHC;IHC:1:50-1:100

ADD3 Antibody

CSB-PA964364-
EUR 335
Description: A polyclonal antibody against ADD3. Recognizes ADD3 from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, WB;WB:1:500-1:3000

ADD3 Antibody

CSB-PA964364-100ul 100ul
EUR 316
Description: A polyclonal antibody against ADD3. Recognizes ADD3 from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, WB;WB:1:500-1:3000

TP53 (Ab-315) Antibody

CSB-PA964395-
EUR 335
Description: A polyclonal antibody against TP53 (Ab-315). Recognizes TP53 (Ab-315) from Human. This antibody is Unconjugated. Tested in the following application: ELISA, WB;WB:1:500-1:1000

TP53 (Ab-315) Antibody

CSB-PA964395-100ul 100ul
EUR 316
Description: A polyclonal antibody against TP53 (Ab-315). Recognizes TP53 (Ab-315) from Human. This antibody is Unconjugated. Tested in the following application: ELISA, WB;WB:1:500-1:1000

PRLR Antibody

CSB-PA964526-
EUR 335
Description: A polyclonal antibody against PRLR. Recognizes PRLR from Human. This antibody is Unconjugated. Tested in the following application: ELISA, WB;WB:1:500-1:1000

PRLR Antibody

CSB-PA964526-100ul 100ul
EUR 316
Description: A polyclonal antibody against PRLR. Recognizes PRLR from Human. This antibody is Unconjugated. Tested in the following application: ELISA, WB;WB:1:500-1:1000

PPP2R3C Antibody

CSB-PA964594-
EUR 335
Description: A polyclonal antibody against PPP2R3C. Recognizes PPP2R3C from Human. This antibody is Unconjugated. Tested in the following application: ELISA, WB;WB:1:500-1:3000

PPP2R3C Antibody

CSB-PA964594-100ul 100ul
EUR 316
Description: A polyclonal antibody against PPP2R3C. Recognizes PPP2R3C from Human. This antibody is Unconjugated. Tested in the following application: ELISA, WB;WB:1:500-1:3000

BAGE2 Antibody

CSB-PA964722-
EUR 335
Description: A polyclonal antibody against BAGE2. Recognizes BAGE2 from Human. This antibody is Unconjugated. Tested in the following application: ELISA, IHC;IHC:1:50-1:100

BAGE2 Antibody

CSB-PA964722-100ul 100ul
EUR 316
Description: A polyclonal antibody against BAGE2. Recognizes BAGE2 from Human. This antibody is Unconjugated. Tested in the following application: ELISA, IHC;IHC:1:50-1:100

PIK3R1/PIK3R3 (Ab-467/199) Antibody

CSB-PA965833-
EUR 335
Description: A polyclonal antibody against PIK3R1/PIK3R3 (Ab-467/199). Recognizes PIK3R1/PIK3R3 (Ab-467/199) from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, IHC;IHC:1:50-1:100

PIK3R1/PIK3R3 (Ab-467/199) Antibody

CSB-PA965833-100ul 100ul
EUR 316
Description: A polyclonal antibody against PIK3R1/PIK3R3 (Ab-467/199). Recognizes PIK3R1/PIK3R3 (Ab-467/199) from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, IHC;IHC:1:50-1:100

MAP2K1 (Ab-291) Antibody

CSB-PA965933-
EUR 335
Description: A polyclonal antibody against MAP2K1 (Ab-291). Recognizes MAP2K1 (Ab-291) from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, WB, IHC;WB:1:500-1:1000, IHC:1:50-1:200

MAP2K1 (Ab-291) Antibody

CSB-PA965933-100ul 100ul
EUR 316
Description: A polyclonal antibody against MAP2K1 (Ab-291). Recognizes MAP2K1 (Ab-291) from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, WB, IHC;WB:1:500-1:1000, IHC:1:50-1:200

Phospho-HSF1 (Ser307) Antibody

CSB-PA966483-
EUR 335
Description: A polyclonal antibody against Phospho-HSF1 (Ser307). Recognizes Phospho-HSF1 (Ser307) from Human. This antibody is Unconjugated. Tested in the following application: ELISA, WB, IF;WB:1:500-1:1000, IF:1:100-1:200

Phospho-HSF1 (Ser307) Antibody

CSB-PA966483-100ul 100ul
EUR 362
Description: A polyclonal antibody against Phospho-HSF1 (Ser307). Recognizes Phospho-HSF1 (Ser307) from Human. This antibody is Unconjugated. Tested in the following application: ELISA, WB, IF;WB:1:500-1:1000, IF:1:100-1:200

ASCL2 Antibody

CSB-PA966616-
EUR 335
Description: A polyclonal antibody against ASCL2. Recognizes ASCL2 from Human. This antibody is Unconjugated. Tested in the following application: ELISA, WB;WB:1:500-1:3000

ASCL2 Antibody

CSB-PA966616-100ul 100ul
EUR 316
Description: A polyclonal antibody against ASCL2. Recognizes ASCL2 from Human. This antibody is Unconjugated. Tested in the following application: ELISA, WB;WB:1:500-1:3000

HNRNPC Antibody

CSB-PA966696-
EUR 335
Description: A polyclonal antibody against HNRNPC. Recognizes HNRNPC from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, WB, IHC, IF;WB:1:500-1:3000, IHC:1:50-1:100, IF:1:100-1:500

HNRNPC Antibody

CSB-PA966696-100ul 100ul
EUR 316
Description: A polyclonal antibody against HNRNPC. Recognizes HNRNPC from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, WB, IHC, IF;WB:1:500-1:3000, IHC:1:50-1:100, IF:1:100-1:500

GZMH Antibody

CSB-PA966723-
EUR 335
Description: A polyclonal antibody against GZMH. Recognizes GZMH from Human. This antibody is Unconjugated. Tested in the following application: ELISA, WB, IF;WB:1:500-1:3000, IF:1:100-1:500

GZMH Antibody

CSB-PA966723-100ul 100ul
EUR 316
Description: A polyclonal antibody against GZMH. Recognizes GZMH from Human. This antibody is Unconjugated. Tested in the following application: ELISA, WB, IF;WB:1:500-1:3000, IF:1:100-1:500

Phospho-SYT1 (Ser309) Antibody

CSB-PA967008-
EUR 335
Description: A polyclonal antibody against Phospho-SYT1 (Ser309). Recognizes Phospho-SYT1 (Ser309) from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, IF;IF:1:100-1:200

Phospho-SYT1 (Ser309) Antibody

CSB-PA967008-100ul 100ul
EUR 362
Description: A polyclonal antibody against Phospho-SYT1 (Ser309). Recognizes Phospho-SYT1 (Ser309) from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, IF;IF:1:100-1:200

NRSN1 Antibody

CSB-PA967502-
EUR 335
Description: A polyclonal antibody against NRSN1. Recognizes NRSN1 from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, WB;WB:1:500-1:3000

NRSN1 Antibody

CSB-PA967502-100ul 100ul
EUR 316
Description: A polyclonal antibody against NRSN1. Recognizes NRSN1 from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, WB;WB:1:500-1:3000

RABL2A Antibody

CSB-PA967554-
EUR 335
Description: A polyclonal antibody against RABL2A. Recognizes RABL2A from Human. This antibody is Unconjugated. Tested in the following application: ELISA, WB;WB:1:500-1:3000

RABL2A Antibody

CSB-PA967554-100ul 100ul
EUR 316
Description: A polyclonal antibody against RABL2A. Recognizes RABL2A from Human. This antibody is Unconjugated. Tested in the following application: ELISA, WB;WB:1:500-1:3000

HDAC3 Antibody

CSB-PA967736-
EUR 335
Description: A polyclonal antibody against HDAC3. Recognizes HDAC3 from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, WB, IHC, IF;WB:1:500-1:3000, IHC:1:50-1:100, IF:1:100-1:500

HDAC3 Antibody

CSB-PA967736-100ul 100ul
EUR 316
Description: A polyclonal antibody against HDAC3. Recognizes HDAC3 from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, WB, IHC, IF;WB:1:500-1:3000, IHC:1:50-1:100, IF:1:100-1:500

AKR1A1 Antibody

CSB-PA967994-
EUR 335
Description: A polyclonal antibody against AKR1A1. Recognizes AKR1A1 from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, WB;WB:1:500-1:3000

AKR1A1 Antibody

CSB-PA967994-100ul 100ul
EUR 316
Description: A polyclonal antibody against AKR1A1. Recognizes AKR1A1 from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, WB;WB:1:500-1:3000

CLCN4 Antibody

CSB-PA968321-
EUR 335
Description: A polyclonal antibody against CLCN4. Recognizes CLCN4 from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, WB, IHC, IF;WB:1:500-1:3000, IHC:1:50-1:200, IF:1:100-1:200

CLCN4 Antibody

CSB-PA968321-100ul 100ul
EUR 316
Description: A polyclonal antibody against CLCN4. Recognizes CLCN4 from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, WB, IHC, IF;WB:1:500-1:3000, IHC:1:50-1:200, IF:1:100-1:200

ATF1 Antibody

CSB-PA968509-
EUR 335
Description: A polyclonal antibody against ATF1. Recognizes ATF1 from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, WB, IF;WB:1:500-1:3000, IF:1:100-1:500

ATF1 Antibody

CSB-PA968509-100ul 100ul
EUR 316
Description: A polyclonal antibody against ATF1. Recognizes ATF1 from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, WB, IF;WB:1:500-1:3000, IF:1:100-1:500

Phospho-MEF2A (Ser408) Antibody

CSB-PA968646-
EUR 335
Description: A polyclonal antibody against Phospho-MEF2A (Ser408). Recognizes Phospho-MEF2A (Ser408) from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, IHC;IHC:1:50-1:100

Phospho-MEF2A (Ser408) Antibody

CSB-PA968646-100ul 100ul
EUR 362
Description: A polyclonal antibody against Phospho-MEF2A (Ser408). Recognizes Phospho-MEF2A (Ser408) from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, IHC;IHC:1:50-1:100

WISP2 Antibody

CSB-PA968927-
EUR 335
Description: A polyclonal antibody against WISP2. Recognizes WISP2 from Human. This antibody is Unconjugated. Tested in the following application: ELISA, WB;WB:1:500-1:1000

WISP2 Antibody

CSB-PA968927-100ul 100ul
EUR 316
Description: A polyclonal antibody against WISP2. Recognizes WISP2 from Human. This antibody is Unconjugated. Tested in the following application: ELISA, WB;WB:1:500-1:1000

Phospho-GRIN2B (Tyr1474) Antibody

CSB-PA969425-
EUR 335
Description: A polyclonal antibody against Phospho-GRIN2B (Tyr1474). Recognizes Phospho-GRIN2B (Tyr1474) from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, WB, IF;WB:1:500-1:1000, IF:1:100-1:200

Phospho-GRIN2B (Tyr1474) Antibody

CSB-PA969425-100ul 100ul
EUR 362
Description: A polyclonal antibody against Phospho-GRIN2B (Tyr1474). Recognizes Phospho-GRIN2B (Tyr1474) from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, WB, IF;WB:1:500-1:1000, IF:1:100-1:200

PMAIP1 Antibody

CSB-PA969433-
EUR 335
Description: A polyclonal antibody against PMAIP1. Recognizes PMAIP1 from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, IHC;IHC:1:50-1:100

PMAIP1 Antibody

CSB-PA969433-100ul 100ul
EUR 316
Description: A polyclonal antibody against PMAIP1. Recognizes PMAIP1 from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, IHC;IHC:1:50-1:100

Phospho-ZAP70 (Tyr319) Antibody

CSB-PA969675-
EUR 335
Description: A polyclonal antibody against Phospho-ZAP70 (Tyr319). Recognizes Phospho-ZAP70 (Tyr319) from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, WB, IHC, IF;WB:1:500-1:1000, IHC:1:50-1:100, IF:1:100-1:200

Phospho-ZAP70 (Tyr319) Antibody

CSB-PA969675-100ul 100ul
EUR 362
Description: A polyclonal antibody against Phospho-ZAP70 (Tyr319). Recognizes Phospho-ZAP70 (Tyr319) from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, WB, IHC, IF;WB:1:500-1:1000, IHC:1:50-1:100, IF:1:100-1:200

RPS4X Antibody

CSB-PA969688-
EUR 335
Description: A polyclonal antibody against RPS4X. Recognizes RPS4X from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, WB, IF;WB:1:500-1:3000, IF:1:100-1:500

RPS4X Antibody

CSB-PA969688-100ul 100ul
EUR 316
Description: A polyclonal antibody against RPS4X. Recognizes RPS4X from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, WB, IF;WB:1:500-1:3000, IF:1:100-1:500

TUBB3 Antibody

CSB-PA969690-
EUR 335
Description: A polyclonal antibody against TUBB3. Recognizes TUBB3 from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, WB, IHC;WB:1:500-1:3000, IHC:1:50-1:100

TUBB3 Antibody

CSB-PA969690-100ul 100ul
EUR 316
Description: A polyclonal antibody against TUBB3. Recognizes TUBB3 from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, WB, IHC;WB:1:500-1:3000, IHC:1:50-1:100

NT5C1B Antibody

CSB-PA969848-
EUR 335
Description: A polyclonal antibody against NT5C1B. Recognizes NT5C1B from Human. This antibody is Unconjugated. Tested in the following application: ELISA, IF;IF:1:100-1:500

NT5C1B Antibody

CSB-PA969848-100ul 100ul
EUR 316
Description: A polyclonal antibody against NT5C1B. Recognizes NT5C1B from Human. This antibody is Unconjugated. Tested in the following application: ELISA, IF;IF:1:100-1:500

AKT1 (Ab-450) Antibody

CSB-PA969941-
EUR 335
Description: A polyclonal antibody against AKT1 (Ab-450). Recognizes AKT1 (Ab-450) from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, WB, IHC, IF;WB:1:500-1:1000, IHC:1:50-1:200, IF:1:100-1:200

AKT1 (Ab-450) Antibody

CSB-PA969941-100ul 100ul
EUR 316
Description: A polyclonal antibody against AKT1 (Ab-450). Recognizes AKT1 (Ab-450) from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, WB, IHC, IF;WB:1:500-1:1000, IHC:1:50-1:200, IF:1:100-1:200

Phospho-MAP2K4 (Ser80) Antibody

CSB-PA970074-
EUR 335
Description: A polyclonal antibody against Phospho-MAP2K4 (Ser80). Recognizes Phospho-MAP2K4 (Ser80) from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, WB, IHC, IF;WB:1:500-1:1000, IHC:1:50-1:100, IF:1:100-1:200

Phospho-MAP2K4 (Ser80) Antibody

CSB-PA970074-100ul 100ul
EUR 362
Description: A polyclonal antibody against Phospho-MAP2K4 (Ser80). Recognizes Phospho-MAP2K4 (Ser80) from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, WB, IHC, IF;WB:1:500-1:1000, IHC:1:50-1:100, IF:1:100-1:200

RAB29 Antibody

CSB-PA970360-
EUR 335
Description: A polyclonal antibody against RAB29. Recognizes RAB29 from Human, Mouse. This antibody is Unconjugated. Tested in the following application: ELISA, WB;WB:1:500-1:3000

RAB29 Antibody

CSB-PA970360-100ul 100ul
EUR 316
Description: A polyclonal antibody against RAB29. Recognizes RAB29 from Human, Mouse. This antibody is Unconjugated. Tested in the following application: ELISA, WB;WB:1:500-1:3000

KIR2DL5B Antibody

CSB-PA970470-
EUR 335
Description: A polyclonal antibody against KIR2DL5B. Recognizes KIR2DL5B from Human. This antibody is Unconjugated. Tested in the following application: ELISA, IF;IF:1:100-1:500

KIR2DL5B Antibody

CSB-PA970470-100ul 100ul
EUR 316
Description: A polyclonal antibody against KIR2DL5B. Recognizes KIR2DL5B from Human. This antibody is Unconjugated. Tested in the following application: ELISA, IF;IF:1:100-1:500

IKZF3 Antibody

CSB-PA970658-
EUR 335
Description: A polyclonal antibody against IKZF3. Recognizes IKZF3 from Human, Mouse. This antibody is Unconjugated. Tested in the following application: ELISA, WB;WB:1:500-1:3000

IKZF3 Antibody

CSB-PA970658-100ul 100ul
EUR 316
Description: A polyclonal antibody against IKZF3. Recognizes IKZF3 from Human, Mouse. This antibody is Unconjugated. Tested in the following application: ELISA, WB;WB:1:500-1:3000

USP13 Antibody

CSB-PA970742-
EUR 335
Description: A polyclonal antibody against USP13. Recognizes USP13 from Human. This antibody is Unconjugated. Tested in the following application: ELISA, WB, IHC;WB:1:500-1:3000, IHC:1:50-1:100

USP13 Antibody

CSB-PA970742-100ul 100ul
EUR 316
Description: A polyclonal antibody against USP13. Recognizes USP13 from Human. This antibody is Unconjugated. Tested in the following application: ELISA, WB, IHC;WB:1:500-1:3000, IHC:1:50-1:100

ABCD4 Antibody

CSB-PA970746-
EUR 335
Description: A polyclonal antibody against ABCD4. Recognizes ABCD4 from Human. This antibody is Unconjugated. Tested in the following application: ELISA, WB;WB:1:500-1:3000

ABCD4 Antibody

CSB-PA970746-100ul 100ul
EUR 316
Description: A polyclonal antibody against ABCD4. Recognizes ABCD4 from Human. This antibody is Unconjugated. Tested in the following application: ELISA, WB;WB:1:500-1:3000

AATF Antibody

CSB-PA970851-
EUR 335
Description: A polyclonal antibody against AATF. Recognizes AATF from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, WB;WB:1:500-1:3000

AATF Antibody

CSB-PA970851-100ul 100ul
EUR 316
Description: A polyclonal antibody against AATF. Recognizes AATF from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, WB;WB:1:500-1:3000

MOB1B Antibody

CSB-PA970943-
EUR 335
Description: A polyclonal antibody against MOB1B. Recognizes MOB1B from Human, Mouse. This antibody is Unconjugated. Tested in the following application: ELISA, WB;WB:1:500-1:3000

MOB1B Antibody

CSB-PA970943-100ul 100ul
EUR 316
Description: A polyclonal antibody against MOB1B. Recognizes MOB1B from Human, Mouse. This antibody is Unconjugated. Tested in the following application: ELISA, WB;WB:1:500-1:3000

PLG Antibody

CSB-PA970985-
EUR 335
Description: A polyclonal antibody against PLG. Recognizes PLG from Human. This antibody is Unconjugated. Tested in the following application: ELISA, WB;WB:1:500-1:3000

PLG Antibody

CSB-PA970985-100ul 100ul
EUR 316
Description: A polyclonal antibody against PLG. Recognizes PLG from Human. This antibody is Unconjugated. Tested in the following application: ELISA, WB;WB:1:500-1:3000

OXR1 Antibody

CSB-PA971107-
EUR 335
Description: A polyclonal antibody against OXR1. Recognizes OXR1 from Human, Mouse. This antibody is Unconjugated. Tested in the following application: ELISA, IHC;IHC:1:50-1:100

OXR1 Antibody

CSB-PA971107-100ul 100ul
EUR 316
Description: A polyclonal antibody against OXR1. Recognizes OXR1 from Human, Mouse. This antibody is Unconjugated. Tested in the following application: ELISA, IHC;IHC:1:50-1:100

NBPF5P Antibody

CSB-PA971232-
EUR 335
Description: A polyclonal antibody against NBPF5P. Recognizes NBPF5P from Human. This antibody is Unconjugated. Tested in the following application: ELISA, WB;WB:1:500-1:3000

NBPF5P Antibody

CSB-PA971232-100ul 100ul
EUR 316
Description: A polyclonal antibody against NBPF5P. Recognizes NBPF5P from Human. This antibody is Unconjugated. Tested in the following application: ELISA, WB;WB:1:500-1:3000

KCNJ4 Antibody

CSB-PA971432-
EUR 335
Description: A polyclonal antibody against KCNJ4. Recognizes KCNJ4 from Human. This antibody is Unconjugated. Tested in the following application: ELISA, WB;WB:1:500-1:3000

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