Preparation of a highly specific polyclonal antibody against and its use in development of ELISA

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The prokaryotic expression, polyclonal antibody preparation, and subcellular localization of the transmembrane protein NS2A of the duck Tembusu virus

 

Duck Tembusu virus (DTMUV) is a single-stranded, positive-sense RNA arbovirus, belonging to the genus Flavivirus, household Flaviviridae. As a transmembrane protein, non-structural protein 2A (NS2A) performs an vital position in virion meeting, replication advanced and antagonizing host immune response. Since NS2A protein comprises many hydrophobic amino acids, it’s arduous to realize the full-length protein of NS2A for prokaryotic expression. Subsequently, to make a deep examine, prokaryotic expression and polyclonal antibody preparation of truncated DTMUV NS2A was carried out.

The truncated NS2A gene (178-450 bp) was obtained, and sub-cloned into the prokaryotic vector pGEX-4T-1 (pGEX-4T-1-NS2A178-450bp). Subsequently, the recombinant GST-NS2A60-150aa protein was efficiently expressed in E. coli BL21 (DE3) with the induction by 0.Three mmol/l isopropyl β-D-thiogalactoside (IPTG) for six h at 37°C. The GST-NS2A60-150aa protein was extracted from the gel. The BALB/c mice have been immunized with the purified recombinant NS2A protein to organize polyclonal antibodies in opposition to the truncated NS2A protein.

The titer of the polyclonal antibodies, decided by enzyme-linked immunosorbent assay (ELISA) evaluation, was 1:128 000. The specificity of the polyclonal antibodies (mPAb-DTMUV-NS2A60-150aa) have been verified by Western blot evaluation. Moreover, the oblique immunofluorescence (IFA) was carried out to discover the subcellular localization of NS2A. NS2A protein was, within the transfected cells, positioned primarily round nucleus within the endoplasmatic reticulum.

Taken collectively, our examine supplied a helpful software for the additional exploration of the organic features and molecular mechanism of DTMUV NS2A. Key phrases: duck Tembusu virus; non-structural protein 2A; prokaryotic expression; polyclonal antibodies; subcellular location.

Prokaryotic expression of mouse Stra8 and preparation and identification of rabbit anti-mouse Stra8 polyclonal antibody

 

Goal To prokaryotically categorical mouse stimulated by retinoic acid gene 8 (Stra8) and put together rabbit anti-mouse Stra8 polyclonal antibody. Strategies The recombinant plasmid pET28a-Stra8 was constructed by cloning expertise and recognized by double enzyme digestion and sequencing, after which reworked into E. coli BL21 (DE3). The expression of Stra8 recombinant protein was induced by IPTG. The prokaryotic protein was purified by His-TAG affinity chromatograph after which used to immune New Zealand white rabbits to acquire Stra8 polyclonal antibody. ELISA,

Western blot and immunofluorescence assays have been used to find out the antibody titer, validity and specificity, respectively. Outcomes The recombinant plasmid pET28a-Stra8 was constructed efficiently as double enzyme digestion and sequencing confirmed, and the prokaryotic protein was expressed and purified successfully. The titer of the polyclonal antibody reached 1:106. Western blotting confirmed that the polyclonal antibody may particularly acknowledge native Stra8 protein within the testis.

Immunofluorescence assay revealed that the polyclonal antibody had good reactivity, and will acknowledge Stra8 protein in mouse testis. Conclusion Stra8 prokaryotic protein might be successfully induced in E. coli and particular rabbit anti-Stra8 polyclonal antibody has been ready.

Preparation of a extremely particular polyclonal antibody in opposition to and its use in growth of ELISA for willpower of in plasma

 

For therapeutic monitoring and pharmacokinetic research of the potent hypocholesterolaemic agent fluvastatin (FLV), a particular antibody was required for the event of a delicate enzyme-linked immunosorbent assay (ELISA) for the correct willpower of FLV in plasma. On this examine, a extremely particular polyclonal antibody in opposition to FLV has been ready. FLV was coupled to keyhole limpet hemocyanin (KLH) and bovine serum albumin (BSA) utilizing carbodiimide reagent. FLV-KLH conjugate was used as an immunogen. Feminine 8-weeks previous New Zealand white rabbits have been immunized with an emulsion of FLV-KLH with Freund’s adjuvant.

The immune response of the rabbits was monitored by direct ELISA utilizing FLV-BSA immobilized onto microwell plates as a strong section. The rabbit that confirmed the best antibody titer and affinity to FLV was scarified and its sera have been collected. The IgG fraction was remoted and purified by affinity chromatography on a protein A column.

The specificity of the purified antibody for FLV was evaluated by oblique aggressive ELISA utilizing numerous rivals from the FLV-structural analogues and therapeutic brokers used with FLV in a mix remedy. The excessive affinity of the antibody (IC50 = 150 pg ml-1) enabled the willpower of FLV in plasma at concentrations as little as 20 pg ml-1.

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Cat IgM-Biotin conjugate (non-immune, isotype control) purified

20002-5-B 0.1 mg
EUR 225

Cat IgM, unlabeled (non-immune, isotype control) purified

20002-5-UL 0.1 mg
EUR 164

FluoroQuest™ Anti-fading Kit II *Optimized for Plate Imaging*

20003 1 kit
EUR 132
  • R-phrase: R20, R21, R22
  • H-Phrase: H303, H313, H333
  • Symbol for dangerous compounds: Xn
  • UNSPEC Code: 12352200

Hamster IgG, purified (Syrian, isotype control)

20003-1 1 mg
EUR 141

Hamster IgG-Biotin conjugate, isotype control (Syrian)

20003-1-B 100 test
EUR 164

Hamster IgG-Cy5 conjugate, isotype control (Syrian)

20003-1-Cy5 50 tests
EUR 225

Hamster IgG-FITC conjugate, isotype control (Syrian)

20003-1-F 100 tests
EUR 164

Hamster IgG-HRP conjugate, isotype control (Syrian)

20003-1-HP 100 tests
EUR 164

Hamster IgG-R-PE-Cy5.5 conjugate, isotype control (Syrian)

20003-1-PC5 50 tests
EUR 250

Hamster IgG-R-PE conjugate, isotype control (Syrian)

20003-1-PE 50 tests
EUR 225

Hamster IgG, purified (Armenian, Isotype control)

20003-1AH 1 mg
EUR 263

Hamster (Syrian, non-immune) Serum IgM, purified (Syrian)

20003-2-1 0.5 mg
EUR 225

FluoroQuest™ Mounting Medium with DAPI

20004 50 mL
EUR 132
  • R-phrase: R20, R21, R68
  • H-Phrase: H303, H313, H340
  • Symbol for dangerous compounds: T
  • UNSPEC Code: 12352200

G. Pig IgG, purified (non-immune, serum, Isotype control)

20004-1 1 mg
EUR 141

G. Pig IgM, purified (non-immune, serum, Isotype control)

20004-2-1 0.5 mg
EUR 225

G. Pig IgG Fc-Biotin conjugate (isotype control, non-immune) purified

20004-3-B 0.1 mg
EUR 225

G. Pig IgG Fc-FITC conjugate (isotype control, non-immune) purified

20004-3-F 0.1 mg
EUR 225

G. Pig IgG Fc-HRP conjugate (isotype control, non-immune) purified

20004-3-HP 0.1 mg
EUR 225

G. Pig IgG Fc unlabeled (isotype control, non-immune) purified

20004-3-UL 0.5 mg
EUR 202

G. Pig IgA, purified (non-immune, serum, Isotype control)

20004-5-1 100 ug
EUR 347

G. Pig IgG-Biotin conjugate (isotype control)

20004-B 100 ug
EUR 202

G. Pig IgG-FITC conjugate (isotype control)

20004-F 100 ug
EUR 202

G. Pig IgG-HRP conjugate (isotype control)

20004-HP 100 ug
EUR 202

Rat IgG, purified (Whole, non-immune) (isotype control)

20005-1 1 mg
EUR 141

Rat IgG purified (isotype control)

20005-1-200 0.5 ml
EUR 103

Rat IgG Fab fragment, purified (isotype control)

20005-1-FAB 1 mg
EUR 164

Rat IgG F(ab')2 fragment, purified (isotype control)

20005-1-FAB2 1 mg
EUR 202

Rat IgG (Fc) fragment, purified (isotype control)

20005-1-FC 0.5 mg
EUR 250

Rat IgG (Fc)-Biotin Conjuagte (isotype control), purified

20005-1-FC-B 0.1 mg
EUR 225

Rat IgG (Fc)-FITC Conjuagte (isotype control), purified

20005-1-FC-F 0.1 mg
EUR 225

Rat IgG (Fc)-HRP Conjuagte (isotype control), purified

20005-1-FC-HP 0.1 mg
EUR 225

Rat IgG, purified (Whole, non-immune) (isotype control)

20005-10 10 mg
EUR 408

Rat IgG1 cunonjugated (isotype control)

20005-11 100 ug
EUR 164

Rat IgG1-Biotin conjugate (isotype control)

20005-11-B 100 ug
EUR 202

Rat IgG1-FITC conjugate (isotype control)

20005-11-F 100 ug
EUR 225

Rat IgG1-HRP conjugate (isotype control)

20005-11-HP 100 ug
EUR 202

Rat IgG1-R-PE-Cy5.5 conjugate (isotype control)

20005-11-PC5 25 tests
EUR 202

Rat IgG1-R-PE conjugate (isotype control)

20005-11-PE 25 tests
EUR 202

Rat IgG2a unconjugated (isotype control)

20005-12 100 ug
EUR 164

Rat IgG2a-APC conjugate (isotype control)

20005-12-APC 25 tests
EUR 202

Rat IgG2a-Biotin conjugate (isotype control)

20005-12-B 100 ug
EUR 202

Rat IgG2a-FITC conjugate (isotype control)

20005-12-F 100 ug
EUR 225

Rat IgG2a-HRP conjugate (isotype control)

20005-12-HP 100 ug
EUR 202

Rat IgG2a-R-PE-Cy5.5 conjugate (isotype control)

20005-12-PC5 25 tests
EUR 213

Rat IgG2a-R-PE conjugate (isotype control)

20005-12-PE 25 tests
EUR 202

Rat IgG2b unconjugated (isotype control)

20005-13 100 ug
EUR 164

Rat IgG2b-Biotin conjugate (isotype control)

20005-13-B 100 ug
EUR 202

Rat IgG2b-FITC conjugate (isotype control)

20005-13-F 100 ug
EUR 225

Rat IgG2b-HRP conjugate (isotype control)

20005-13-HP 100 ug
EUR 202

Rat IgG2b-R-PE-Cy5.5 conjugate (isotype control)

20005-13-PC5 25 tests
EUR 213

Rat IgG2b-R-PE conjugate (isotype control)

20005-13-PE 25 tests
EUR 202

Rat IgG2c-Biotin conjugate (isotype control)

20005-14-B 100 ug
EUR 225

Rat IgG2c-FITC conjugate (isotype control)

20005-14-F 100 ug
EUR 225

Rat IgG2c-HRP conjugate (isotype control)

20005-14-HP 100 ug
EUR 225

Rat IgG2c-R-PE conjugate (isotype control)

20005-14-PE 50 tests
EUR 250

Rat IgG2c unonjugated (isotype control)

20005-14-UL 100 ug
EUR 164

Rat IgM (non-immune), purified (isotype control)

20005-2-1 0.1 mg
EUR 164

Rat IgM-Biotin conjugate (isotype control)

20005-21-B 100 ug
EUR 225

Rat IgM-FITC conjugate (isotype control)

20005-21-F 100 ug
EUR 225

Rat IgM-HRP conjugate (isotype control)

20005-21-HP 100 ug
EUR 225

Rat IgM-R-PE conjugate (isotype control)

20005-21-PE 25 tests
EUR 202

Rat IgA (non-immune), purified (isotype control)

20005-3-1 25 ug
EUR 225

Rat IgG, purified (Isotype control)

20005-5 5 mg
EUR 286

Rat IgG-Agarose conjugate(aff matrix)

20005-AS-1 0.5 ml
EUR 164

Rat IgG-Biotin conjugate (isotype control) (Isotype control)

20005-B 100 ug
EUR 164

Rat IgG-FITC conjugate (isotype control) (Isotype control)

20005-F 100 ug
EUR 164

Rat IgG-HRP conjugate (isotype control) (Isotype control)

20005-HP 100 ug
EUR 164

Rat IgG-PE conjugate (isotype control) (Isotype control)

20005-PE 25 tests
EUR 202

FluoroQuest™ Fluorescence Signal Enhancing Solution

20006 5 mL
EUR 132
  • R-phrase: R20, R21, R68
  • H-Phrase: H303, H313, H340
  • Symbol for dangerous compounds: T
  • UNSPEC Code: 12352200

Sheep IgG, purified (isotype control)

20006-1 1 mg
EUR 141

Sheep IgG-Biotin Conjugate (isotype control, non-immune), purified

20006-1-B 0.5 mg
EUR 202

Sheep IgG-FITC Conjugate (isotype control, non-immune), purified

20006-1-F 0.5 mg
EUR 202

Sheep IgG-HRP Conjugate (isotype control, non-immune), purified

20006-1-HP 0.5 mg
EUR 202

Sheep IgM purified (isotype control)

20006-2 1 mg
EUR 347

Sheep IgM-Biotin Conjugate (non-immune) control, purified

20006-2-B 0.1 mg
EUR 225

Sheep IgA purified (isotype control)

20006-3 100 ug
EUR 286

Sheep IgG Fc-Biotin Conjugate (isotype control, non-immune), purified

20006-4-B 0.5 mg
EUR 202

Sheep IgG Fc-FITC Conjugate (isotype control, non-immune), purified

20006-4-F 0.5 mg
EUR 202

Sheep IgG Fc-HRP Conjugate (isotype control, non-immune), purified

20006-4-HP 0.5 mg
EUR 202

Sheep IgG Fc unlabeled (isotype control, non-immune), purified

20006-4-UL 0.5 mg
EUR 202

Human IgG, purified (serum, non-immune, isotype control)

20007-1-1 1 mg
EUR 141

Human IgG, purified (serum, non-immune, isotype control)

20007-1-100 100 mg
EUR 895

Human IgG, purified (serum, non-immune, isotype control)

20007-1-25 25 mg
EUR 651

Human IgG, purified (serum, non-immune, isotype control)

20007-1-5 5 mg
EUR 286

Human IgG-Biotin conjugate (isotype control, non-immune), purified

20007-1-B 0.5 mg
EUR 225

Human IgG (>98%, non-immune, control, Liquid @ 10 mg/ml, azide free, bulk size)

20007-1-BL-1 10 ml Ask for price

Human IgG (>98%, non-immune, control, powder, azide free, bulk size)

20007-1-BP-1 1 g
EUR 651

Human IgG (>98%, non-immune, control, powder, azide free, bulk size)

20007-1-BP-10 10 g
EUR 4313

Human IgG-FITC conjugate (isotype control, non-immune), purified

20007-1-F 0.5 mg
EUR 225

Human IgG Fab fragment, purified

20007-1-FAB 1 mg
EUR 202

Human IgG Fab fragment-Biotin Conjugate, purified

20007-1-FAB-B 0.1 mg
EUR 202

Human IgG Fab fragment-FITC Conjugate, purified

20007-1-FAB-F 0.1 mg
EUR 202

Human IgG Fab fragment-HRP Conjugate, purified

20007-1-FAB-HP 0.1 mg
EUR 202

Human IgG F(ab')2 fragment, purified

20007-1-FAB2 1 mg
EUR 202

Human IgG Fab2 fragment-Biotin Conjugate, purified

20007-1-FAB2-B 0.1 mg
EUR 202

Human IgG Fab2 fragment-FITC Conjugate, purified

20007-1-FAB2-F 0.1 mg
EUR 202

Human IgG Fab2 fragment-HRP Conjugate, purified

20007-1-FAB2-HP 0.1 mg
EUR 202

Human IgG (Fc) fragment, purified

20007-1-FC 0.5 mg
EUR 208

Human IgG Fc-Biotin conjugate (isotype control, non-immune), purified

20007-1-Fc-B 0.1 mg
EUR 164

Human IgG Fc-FITC conjugate (isotype control, non-immune), purified

20007-1-Fc-F 0.1 mg
EUR 164

Human IgG Fc-HRP conjugate (isotype control, non-immune), purified

20007-1-Fc-HP 0.1 mg
EUR 164

Human IgG (Fc) fragment, purified (>95%, low endotoxin)

20007-1-FC-LE 100 ul
EUR 225

Human IgG (Fc) fragment-Biotin purified (>95%, low endotoxin)

20007-1-FC-LE-B 25 ug
EUR 286

Human IgG-HRP Conjugate (isotype control)

20007-1-HP 500 ug
EUR 202

Human IgG (serum origin, purified >97%, low endotoxin, azide free)

20007-1-LE-1 1 mg
EUR 164

Human IgG-Biotin (serum origin, purified >97%, low endotoxin, azide free)

20007-1-LE-BTN 100 ug
EUR 286

TruStrip RDT Human IgG Rapid test cards, 10/pk

20007-1-RDT 1 Pk
EUR 171

TruStrip RDT Human IgG Rapid test cards, 25/pk

20007-1-RDT-25 1 Pk
EUR 293

Human IgM, purified (native, isotype control, >96% pure, ELISA Grade)

20007-2 1 mg
EUR 164

Human IgM purified (isotype control, >96% pure, ELISA Grade)

20007-2-10 10 mg
EUR 895

polyclonal antibody in opposition to a recombinantly expressed Triticum aestivum RHT-D1A protein

 

Background: Lowered height-1 dwarfing alleles have an effect on DELLA proteins belonging to a household of putative transcriptional regulators that modulate plant development and growth. The Arabidopsis thaliana genome encodes 5 DELLA proteins, whereas monocot crops, reminiscent of rice, barley, and wheat, every have a single DELLA protein. In wheat, wild-type Rht-B1a and Rht-D1a genes encode DELLA proteins and have many alleles that comprise lesions.

 

Amongst them, Rht-B1b and Rht-D1b are the commonest mutant dwarfing alleles, which have performed a key half within the creation of high-yielding wheat varieties. Regardless of their basic roles in plant biology, till now, DELLA proteins in wheat have been primarily researched concerning the phenotypic impact of faulty Rht mutants on yield-related traits, with out research on the underlying mechanisms.

The RHT-1 protein has but to be detected in wheat tissues, owing to a scarcity of applicable molecular instruments for characterization of RHT perform and protein interactions in sign transduction. This examine is concentrated on the manufacturing of a polyclonal antibody to the wheat RHT-D1A protein.

 

Outcomes: To generate the anti-RHT-D1A antibody, we expressed and purified soluble 6xHis-tagged RHT-D1A. The purified recombinant RHT-D1A was injected into New Zealand white rabbits to generate polyclonal antiserum. The polyclonal anti-RHT-D1A antibody was purified by ammonium sulfate precipitation, adopted by affinity chromatography on protein A-agarose beads.

The purified polyclonal antibody was demonstrated to be efficient in immunoblotting, western blot hybridization, and immunoprecipitation. In wheat seedling extracts, the polyclonal antibody acknowledged a protein with a molecular mass near the expected molecular weight of the endogenous RHT-D1A protein. We additionally demonstrated that RHT-D1A disappears in response to exogenous and endogenous gibberellic acid.

 

Conclusion: The purified polyclonal antibody raised in opposition to the recombinant RHT-D1A protein is sufficiently particular and delicate and could possibly be a useful gizmo for future insights into upstream and downstream parts of DELLA-regulatory mechanisms in wheat crops.

 

Bacterial expression of 183-227aa area of HER3 extracellular area I and preparation and identification of its polyclonal antibodies

 

Goal: To arrange the recombinant peptide MVF-HER3 I composed of the 183-227aa peptide section of human epidermal development issue receptor 3 (HER3 I) and the measles virus protein 288-302 peptide section (MVF), and put together polyclonal antibodies (PcAb) in opposition to this recombinant peptide.

 

Strategies: The MVF-HER3 I gene was synthesized chemically and subcloned into pET21b or pET32a plasmid containing Thioredoxin (Trx) tag gene. The recombinant plasmids have been recognized by endonuclease digestion. MVF-HER3 I used to be expressed in E.coli BL21(DE3) cells underneath an optimum bacterial expression situation.

The fusion protein Trx-MVF-HER3 I used to be purified utilizing nickel ion affinity chromatography, and the purified protein was digested by enterokinase to take away Trx tag. The digested combination underwent additional nickel ion affinity chromatography to acquire purified MVF-HER3 I.

The purified MVF-HER3 I used to be used to immunize SD rats subcutaneously for getting ready anti-MVF-HER3 I PcAb. The titer of PcAb was decided utilizing ELISA. The bindings of anti-MVF-HER3 I PcAb to MVF-HER3 I, native HER3 and MCF7 cells have been analyzed utilizing immunoblotting, immunoprecipitation and laser confocal microscopy. The expansion inhibition impact of the antibodies on MCF7 cells cultured within the absence or presence of NRG was assessed utilizing sulforhodamine B.

 

Outcomes: The recombinant peptide gene couldn’t be expressed alone, however could possibly be effectively expressed after fusion with Trx gene underneath optimized situations. The fusion peptide MVF-HER3 I used to be efficiently ready from Trx-MVF-HER3 I. The anti-MVF-HER3 I PcAb, with a titer reaching 1: 512 000, particularly sure to MVF-HER3 I, acknowledged native HER3 and sure to the membrane of MCF7 cells. The obtained PcAb may dose-dependently inhibit the expansion of MCF7 cells no matter the presence or absence of NRG.

Cat IgM-Biotin conjugate (non-immune, isotype control) purified
20002-5-B 0.1 mg
EUR 225
Cat IgM, unlabeled (non-immune, isotype control) purified
20002-5-UL 0.1 mg
EUR 164
FluoroQuest™ Anti-fading Kit II *Optimized for Plate Imaging*
20003 1 kit
EUR 132
  • R-phrase: R20, R21, R22
  • H-Phrase: H303, H313, H333
  • Symbol for dangerous compounds: Xn
  • UNSPEC Code: 12352200
Hamster IgG, purified (Syrian, isotype control)
20003-1 1 mg
EUR 141
Hamster IgG-Biotin conjugate, isotype control (Syrian)
20003-1-B 100 test
EUR 164
Hamster IgG-Cy5 conjugate, isotype control (Syrian)
20003-1-Cy5 50 tests
EUR 225
Hamster IgG-FITC conjugate, isotype control (Syrian)
20003-1-F 100 tests
EUR 164
Hamster IgG-HRP conjugate, isotype control (Syrian)
20003-1-HP 100 tests
EUR 164
Hamster IgG-R-PE-Cy5.5 conjugate, isotype control (Syrian)
20003-1-PC5 50 tests
EUR 250
Hamster IgG-R-PE conjugate, isotype control (Syrian)
20003-1-PE 50 tests
EUR 225
Hamster IgG, purified (Armenian, Isotype control)
20003-1AH 1 mg
EUR 263
Hamster (Syrian, non-immune) Serum IgM, purified (Syrian)
20003-2-1 0.5 mg
EUR 225
FluoroQuest™ Mounting Medium with DAPI
20004 50 mL
EUR 132
  • R-phrase: R20, R21, R68
  • H-Phrase: H303, H313, H340
  • Symbol for dangerous compounds: T
  • UNSPEC Code: 12352200
G. Pig IgG, purified (non-immune, serum, Isotype control)
20004-1 1 mg
EUR 141
G. Pig IgM, purified (non-immune, serum, Isotype control)
20004-2-1 0.5 mg
EUR 225
G. Pig IgG Fc-Biotin conjugate (isotype control, non-immune) purified
20004-3-B 0.1 mg
EUR 225
G. Pig IgG Fc-FITC conjugate (isotype control, non-immune) purified
20004-3-F 0.1 mg
EUR 225
G. Pig IgG Fc-HRP conjugate (isotype control, non-immune) purified
20004-3-HP 0.1 mg
EUR 225
G. Pig IgG Fc unlabeled (isotype control, non-immune) purified
20004-3-UL 0.5 mg
EUR 202
G. Pig IgA, purified (non-immune, serum, Isotype control)
20004-5-1 100 ug
EUR 347
G. Pig IgG-Biotin conjugate (isotype control)
20004-B 100 ug
EUR 202
G. Pig IgG-FITC conjugate (isotype control)
20004-F 100 ug
EUR 202
G. Pig IgG-HRP conjugate (isotype control)
20004-HP 100 ug
EUR 202
Rat IgG, purified (Whole, non-immune) (isotype control)
20005-1 1 mg
EUR 141
Rat IgG purified (isotype control)
20005-1-200 0.5 ml
EUR 103
Rat IgG Fab fragment, purified (isotype control)
20005-1-FAB 1 mg
EUR 164
Rat IgG F(ab')2 fragment, purified (isotype control)
20005-1-FAB2 1 mg
EUR 202
Rat IgG (Fc) fragment, purified (isotype control)
20005-1-FC 0.5 mg
EUR 250
Rat IgG (Fc)-Biotin Conjuagte (isotype control), purified
20005-1-FC-B 0.1 mg
EUR 225
Rat IgG (Fc)-FITC Conjuagte (isotype control), purified
20005-1-FC-F 0.1 mg
EUR 225
Rat IgG (Fc)-HRP Conjuagte (isotype control), purified
20005-1-FC-HP 0.1 mg
EUR 225
Rat IgG, purified (Whole, non-immune) (isotype control)
20005-10 10 mg
EUR 408
Rat IgG1 cunonjugated (isotype control)
20005-11 100 ug
EUR 164
Rat IgG1-Biotin conjugate (isotype control)
20005-11-B 100 ug
EUR 202
Rat IgG1-FITC conjugate (isotype control)
20005-11-F 100 ug
EUR 225
Rat IgG1-HRP conjugate (isotype control)
20005-11-HP 100 ug
EUR 202
Rat IgG1-R-PE-Cy5.5 conjugate (isotype control)
20005-11-PC5 25 tests
EUR 202
Rat IgG1-R-PE conjugate (isotype control)
20005-11-PE 25 tests
EUR 202
Rat IgG2a unconjugated (isotype control)
20005-12 100 ug
EUR 164
Rat IgG2a-APC conjugate (isotype control)
20005-12-APC 25 tests
EUR 202
Rat IgG2a-Biotin conjugate (isotype control)
20005-12-B 100 ug
EUR 202
Rat IgG2a-FITC conjugate (isotype control)
20005-12-F 100 ug
EUR 225
Rat IgG2a-HRP conjugate (isotype control)
20005-12-HP 100 ug
EUR 202
Rat IgG2a-R-PE-Cy5.5 conjugate (isotype control)
20005-12-PC5 25 tests
EUR 213
Rat IgG2a-R-PE conjugate (isotype control)
20005-12-PE 25 tests
EUR 202
Rat IgG2b unconjugated (isotype control)
20005-13 100 ug
EUR 164
Rat IgG2b-Biotin conjugate (isotype control)
20005-13-B 100 ug
EUR 202
Rat IgG2b-FITC conjugate (isotype control)
20005-13-F 100 ug
EUR 225
Rat IgG2b-HRP conjugate (isotype control)
20005-13-HP 100 ug
EUR 202
Rat IgG2b-R-PE-Cy5.5 conjugate (isotype control)
20005-13-PC5 25 tests
EUR 213
Rat IgG2b-R-PE conjugate (isotype control)
20005-13-PE 25 tests
EUR 202
Rat IgG2c-Biotin conjugate (isotype control)
20005-14-B 100 ug
EUR 225
Rat IgG2c-FITC conjugate (isotype control)
20005-14-F 100 ug
EUR 225
Rat IgG2c-HRP conjugate (isotype control)
20005-14-HP 100 ug
EUR 225
Rat IgG2c-R-PE conjugate (isotype control)
20005-14-PE 50 tests
EUR 250
Rat IgG2c unonjugated (isotype control)
20005-14-UL 100 ug
EUR 164
Rat IgM (non-immune), purified (isotype control)
20005-2-1 0.1 mg
EUR 164
Rat IgM-Biotin conjugate (isotype control)
20005-21-B 100 ug
EUR 225
Rat IgM-FITC conjugate (isotype control)
20005-21-F 100 ug
EUR 225
Rat IgM-HRP conjugate (isotype control)
20005-21-HP 100 ug
EUR 225
Rat IgM-R-PE conjugate (isotype control)
20005-21-PE 25 tests
EUR 202
Rat IgA (non-immune), purified (isotype control)
20005-3-1 25 ug
EUR 225
Rat IgG, purified (Isotype control)
20005-5 5 mg
EUR 286
Rat IgG-Agarose conjugate(aff matrix)
20005-AS-1 0.5 ml
EUR 164
Rat IgG-Biotin conjugate (isotype control) (Isotype control)
20005-B 100 ug
EUR 164
Rat IgG-FITC conjugate (isotype control) (Isotype control)
20005-F 100 ug
EUR 164
Rat IgG-HRP conjugate (isotype control) (Isotype control)
20005-HP 100 ug
EUR 164
Rat IgG-PE conjugate (isotype control) (Isotype control)
20005-PE 25 tests
EUR 202
FluoroQuest™ Fluorescence Signal Enhancing Solution
20006 5 mL
EUR 132
  • R-phrase: R20, R21, R68
  • H-Phrase: H303, H313, H340
  • Symbol for dangerous compounds: T
  • UNSPEC Code: 12352200
Sheep IgG, purified (isotype control)
20006-1 1 mg
EUR 141
Sheep IgG-Biotin Conjugate (isotype control, non-immune), purified
20006-1-B 0.5 mg
EUR 202
Sheep IgG-FITC Conjugate (isotype control, non-immune), purified
20006-1-F 0.5 mg
EUR 202
Sheep IgG-HRP Conjugate (isotype control, non-immune), purified
20006-1-HP 0.5 mg
EUR 202
Sheep IgM purified (isotype control)
20006-2 1 mg
EUR 347
Sheep IgM-Biotin Conjugate (non-immune) control, purified
20006-2-B 0.1 mg
EUR 225
Sheep IgA purified (isotype control)
20006-3 100 ug
EUR 286
Sheep IgG Fc-Biotin Conjugate (isotype control, non-immune), purified
20006-4-B 0.5 mg
EUR 202
Sheep IgG Fc-FITC Conjugate (isotype control, non-immune), purified
20006-4-F 0.5 mg
EUR 202
Sheep IgG Fc-HRP Conjugate (isotype control, non-immune), purified
20006-4-HP 0.5 mg
EUR 202
Sheep IgG Fc unlabeled (isotype control, non-immune), purified
20006-4-UL 0.5 mg
EUR 202
Human IgG, purified (serum, non-immune, isotype control)
20007-1-1 1 mg
EUR 141
Human IgG, purified (serum, non-immune, isotype control)
20007-1-100 100 mg
EUR 895
Human IgG, purified (serum, non-immune, isotype control)
20007-1-25 25 mg
EUR 651
Human IgG, purified (serum, non-immune, isotype control)
20007-1-5 5 mg
EUR 286
Human IgG-Biotin conjugate (isotype control, non-immune), purified
20007-1-B 0.5 mg
EUR 225
Human IgG (>98%, non-immune, control, Liquid @ 10 mg/ml, azide free, bulk size)
20007-1-BL-1 10 ml Ask for price
Human IgG (>98%, non-immune, control, powder, azide free, bulk size)
20007-1-BP-1 1 g
EUR 651
Human IgG (>98%, non-immune, control, powder, azide free, bulk size)
20007-1-BP-10 10 g
EUR 4313
Human IgG-FITC conjugate (isotype control, non-immune), purified
20007-1-F 0.5 mg
EUR 225
Human IgG Fab fragment, purified
20007-1-FAB 1 mg
EUR 202

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