Evaluation of Laboratory Application of Camelid Sera Containing Heavy-Chain Polyclonal Antibody

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Evaluation of Laboratory Application of Camelid Sera Containing Heavy-Chain Polyclonal Antibody Against Recombinant CTLA-4 Protein. Cytotoxic T-lymphocyte-associated protein-4 (CTLA-4) is a critical negative immunomodulatory receptor that is normally expressed in activated T cells and noticeably, in many cancerous cells. Indeed, molecular detection of CTLA-4 protein is crucial in basic research. In this work, the extracellular domain of the […]

Prokaryotic expression of PE8 protein from Mycobacterium tuberculosis

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Improvement of a Extremely Delicate Enzyme-Linked Immunosorbent Assay for Mouse Soluble Epoxide Hydrolase Detection by Combining a Polyclonal Seize Antibody with a Nanobody Tracer   Enzyme-linked immunosorbent assays (ELISA) for the detection of soluble epoxide hydrolase (sEH), a key enzyme within the metabolism of fatty acids and a biomarker, might more and more signify an essential diagnostic software. Nonetheless, […]

Expression of HPV16 E6 recombinant protein and preparation of its rabbit polyclonal antibody

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Era of Excessive Affinity Anti-Peptide Polyclonal Antibodies Recognizing Goat α s1-Casein   The chemical, technological and allergy properties of goat’s milk are considerably affected by the extent of αs1-casein. Detection and quantification of αs1-casein requires high-specificity strategies to beat high-sequence similarity between this protein and others within the casein household. Unavailability of antibodies with excessive affinity and specificity in […]

Preparation of a highly specific polyclonal antibody against and its use in development of ELISA

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The prokaryotic expression, polyclonal antibody preparation, and subcellular localization of the transmembrane protein NS2A of the duck Tembusu virus   Duck Tembusu virus (DTMUV) is a single-stranded, positive-sense RNA arbovirus, belonging to the genus Flavivirus, household Flaviviridae. As a transmembrane protein, non-structural protein 2A (NS2A) performs an vital position in virion meeting, replication advanced and antagonizing host […]