Evaluation of Laboratory Application of Camelid Sera Containing Heavy-Chain Polyclonal Antibody

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Evaluation of Laboratory Application of Camelid Sera Containing Heavy-Chain Polyclonal Antibody Against Recombinant CTLA-4 Protein. Cytotoxic T-lymphocyte-associated protein-4 (CTLA-4) is a critical negative immunomodulatory receptor that is normally expressed in activated T cells and noticeably, in many cancerous cells. Indeed, molecular detection of CTLA-4 protein is crucial in basic research. In this work, the extracellular domain of the […]

Efficient detection of eukaryotic calcium-sensing receptor

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In direction of middle-up evaluation of polyclonal antibodies: subclass-specific N-glycosylation profiling of murine immunoglobulin G (IgG) by the use of HPLC-MS   Lately, superior HPLC-MS methods based mostly on intact protein (“top-down”) or protein subunit (“middle-up/middle-down”) evaluation have been applied for the characterization of therapeutic monoclonal antibodies. Right here, we assess feasibility of middle-up/middle-down evaluation for polyclonal […]

Prokaryotic expression of PE8 protein from Mycobacterium tuberculosis

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Improvement of a Extremely Delicate Enzyme-Linked Immunosorbent Assay for Mouse Soluble Epoxide Hydrolase Detection by Combining a Polyclonal Seize Antibody with a Nanobody Tracer   Enzyme-linked immunosorbent assays (ELISA) for the detection of soluble epoxide hydrolase (sEH), a key enzyme within the metabolism of fatty acids and a biomarker, might more and more signify an essential diagnostic software. Nonetheless, […]