Prokaryotic expression of PE8 protein from Mycobacterium tuberculosis
Improvement of a Extremely Delicate Enzyme-Linked Immunosorbent Assay for Mouse Soluble Epoxide Hydrolase Detection by Combining a Polyclonal Seize Antibody with a Nanobody Tracer
Enzyme-linked immunosorbent assays (ELISA) for the detection of soluble epoxide hydrolase (sEH), a key enzyme within the metabolism of fatty acids and a biomarker, might more and more signify an essential diagnostic software. Nonetheless, there’s a lack of ELISAs for mouse sEH quantification, thus leading to a bottleneck in understanding the pathogenesis of many ailments associated to sEH based mostly on mouse fashions.
On this work, nanobodies recognizing mouse sEH have been obtained by way of rebiopanning in opposition to mouse sEH within the earlier phage show library of human sEH. Later, we developed 4 ELISAs involving a mix of anti-mouse sEH polyclonal antibodies (pAbs) and nanobodies. It was discovered that the double antibodies labored as twin filters and had a big impact on each the sensitivity and selectivity of sandwich immunoassays.
The change from anti-human sEH pAbs to anti-mouse sEH pAbs led to over a 100-fold improve within the sensitivity and a dramatic lower of the restrict of detection to a picogram per milliliter vary in format B (pAb/biotin-VHH/streptavidin-poly-horseradish peroxidase). Furthermore, we discovered that the 4 sandwich ELISAs would possibly exhibit glorious selectivities to mouse sEH, regardless of the antibodies alone displaying important cross-reactivity to the matrix, indicating the improved selectivity of double antibodies as twin filters.
Finally, for the primary time, the ELISA (format B) was efficiently used to measure the mouse sEH stage in most cancers cells with ultralow abundances. The ELISAs proposed right here signify a delicate software for monitoring sEH in numerous organic processes and likewise present deep insights into creating sandwich immunoassays in opposition to numerous targets when it comes to each the sensitivity and selectivity.
Prediction of Clearance of Monoclonal and Polyclonal Antibodies and Non-Antibody Proteins in Kids: Software of Allometric Scaling
Allometric scaling can be utilized for the extrapolation of pharmacokinetic parameters from adults to kids. The target of this examine was to foretell clearance of therapeutic proteins (monoclonal and polyclonal antibodies and non-antibody proteins) allometrically in preterm neonates to adolescents. There have been 13 monoclonal antibodies, seven polyclonal antibodies, and 9 therapeutic proteins (non-antibodies) within the examine.
The clearance of therapeutic proteins was predicted utilizing the age dependent exponents (ADE) mannequin after which in contrast with the noticed clearance values. There have been in complete 29 therapeutic proteins on this examine with 75 observations. The variety of observations with ≤30%, ≤50%, and >50% prediction error was 60 (80%), 72 (96%), and three (4%), respectively.
General, the anticipated clearance values of therapeutic proteins in kids was good. The allometric methodology proposed on this manuscript can be utilized to pick first-in-pediatric dose of therapeutic proteins in pediatric medical trials.
Fenretinide |
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HY-15373 | MedChemExpress | 10mM/1mL | EUR 151.2 |
Fenretinide |
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F250000 | Toronto Research Chemicals | 25mg | EUR 106 |
Description: 65646-68-6 |
Fenretinide |
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GP8861-10 | Glentham Life Sciences | 10 | EUR 63.3 |
Fenretinide |
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GP8861-10MG | Glentham Life Sciences | 10 mg | EUR 112.8 |
Fenretinide |
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GP8861-25 | Glentham Life Sciences | 25 | EUR 110.7 |
Fenretinide |
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GP8861-25MG | Glentham Life Sciences | 25 mg | EUR 170.4 |
Fenretinide |
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T1872-10mg | TargetMol Chemicals | 10mg | Ask for price |
Description: Fenretinide |
Fenretinide |
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T1872-1g | TargetMol Chemicals | 1g | Ask for price |
Description: Fenretinide |
Fenretinide |
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T1872-1mg | TargetMol Chemicals | 1mg | Ask for price |
Description: Fenretinide |
Fenretinide |
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T1872-50mg | TargetMol Chemicals | 50mg | Ask for price |
Description: Fenretinide |
Fenretinide |
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T1872-5mg | TargetMol Chemicals | 5mg | Ask for price |
Description: Fenretinide |
Fenretinide-d4 |
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F250002 | Toronto Research Chemicals | 25mg | EUR 1200 |
Fenretinide (4-HPR) |
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abx282632-100g | Abbexa | 100 µg | Ask for price |
Fenretinide (4-HPR) |
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abx282632-20g | Abbexa | 20 µg | EUR 143.75 |
Fenretinide (4-HPR) |
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abx282632-50g | Abbexa | 50 µg | EUR 362.5 |
3-Hydroxy Fenretinide |
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H825635 | Toronto Research Chemicals | 50mg | EUR 4500 |
Description: 1027233-22-2 |
Fenretinide [CAS# 65646-68-6] |
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F-4077 | LC LABORATORIES | 10 mg | EUR 39 |
4-Methoxy Fenretinide-13C,d3 |
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M262662 | Toronto Research Chemicals | 25mg | EUR 3000 |
3-Hydroxy Fenretinide-d4 |
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H825637 | Toronto Research Chemicals | 25mg | EUR 4500 |
4-Methoxy Fenretinide-d4 |
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M262661 | Toronto Research Chemicals | 10mg | EUR 1200 |
Fenretinide Glucuronide Monosodium Salt |
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F250010 | Toronto Research Chemicals | 500mg | EUR 11200 |
Description: 76177-99-6 |
Fenretinide Glucuronide Monosodium Salt-d4 |
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F250012 | Toronto Research Chemicals | 100mg | EUR 11200 |
13-cis-Fenretinide |
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F250005 | Toronto Research Chemicals | 100mg | EUR 1455 |
Description: 75686-07-6 |
13-cis-Fenretinide-d4 |
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F250007 | Toronto Research Chemicals | 25mg | EUR 1200 |
Fen1/ Rat Fen1 ELISA Kit |
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ELI-27513r | Lifescience Market | 96 Tests | EUR 1063.2 |
1000 ug/mL Fenthion in Acetone - 1ML |
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S-2010-AC | Scientific Laboratory Supplies | 1ML | EUR 189 |
1000 ug/mL Fenobucarb in Acetone - 1ML |
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S-4610-AC | Scientific Laboratory Supplies | 1ML | EUR 106.65 |
FEN1 |
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CSB-CL008585HU | Cusabio | 10 μg plasmid + 200μl Glycerol | Ask for price |
FEN1 |
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enz-468 | ProSpec Tany | 5µg | EUR 60 |
Description: Recombinant Human Flap Structure-Specific Endonuclease 1 |
1000 ug/mL Fenpyroximate in Acetone - 1ML |
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S-5804 | Scientific Laboratory Supplies | 1ML | EUR 259.2 |
FEN1 siRNA |
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20-abx916746 | Abbexa |
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FEN1 siRNA |
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20-abx916747 | Abbexa |
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FEN1-IN-2 |
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T40931-10mg | TargetMol Chemicals | 10mg | Ask for price |
Description: FEN1-IN-2 |
FEN1-IN-2 |
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T40931-1g | TargetMol Chemicals | 1g | Ask for price |
Description: FEN1-IN-2 |
FEN1-IN-2 |
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T40931-1mg | TargetMol Chemicals | 1mg | Ask for price |
Description: FEN1-IN-2 |
FEN1-IN-2 |
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T40931-50mg | TargetMol Chemicals | 50mg | Ask for price |
Description: FEN1-IN-2 |
FEN1-IN-2 |
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T40931-5mg | TargetMol Chemicals | 5mg | Ask for price |
Description: FEN1-IN-2 |
FEN1-IN-3 |
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T9147-10mg | TargetMol Chemicals | 10mg | Ask for price |
Description: FEN1-IN-3 |
FEN1-IN-3 |
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T9147-1g | TargetMol Chemicals | 1g | Ask for price |
Description: FEN1-IN-3 |
FEN1-IN-3 |
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T9147-1mg | TargetMol Chemicals | 1mg | Ask for price |
Description: FEN1-IN-3 |
FEN1-IN-3 |
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T9147-50mg | TargetMol Chemicals | 50mg | Ask for price |
Description: FEN1-IN-3 |
FEN1-IN-3 |
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T9147-5mg | TargetMol Chemicals | 5mg | Ask for price |
Description: FEN1-IN-3 |
FEN1-IN-1 |
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T15274-10mg | TargetMol Chemicals | 10mg | Ask for price |
Description: FEN1-IN-1 |
FEN1-IN-1 |
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T15274-1g | TargetMol Chemicals | 1g | Ask for price |
Description: FEN1-IN-1 |
FEN1-IN-1 |
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T15274-1mg | TargetMol Chemicals | 1mg | Ask for price |
Description: FEN1-IN-1 |
FEN1-IN-1 |
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T15274-50mg | TargetMol Chemicals | 50mg | Ask for price |
Description: FEN1-IN-1 |
FEN1-IN-1 |
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T15274-5mg | TargetMol Chemicals | 5mg | Ask for price |
Description: FEN1-IN-1 |
FEN1-IN-4 |
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T8545-10mg | TargetMol Chemicals | 10mg | Ask for price |
Description: FEN1-IN-4 |
FEN1-IN-4 |
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T8545-1g | TargetMol Chemicals | 1g | Ask for price |
Description: FEN1-IN-4 |
FEN1-IN-4 |
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T8545-1mg | TargetMol Chemicals | 1mg | Ask for price |
Description: FEN1-IN-4 |
FEN1-IN-4 |
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T8545-50mg | TargetMol Chemicals | 50mg | Ask for price |
Description: FEN1-IN-4 |
FEN1-IN-4 |
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T8545-5mg | TargetMol Chemicals | 5mg | Ask for price |
Description: FEN1-IN-4 |
Lenti ORF particles, Fen1 (GFP-tagged) - Mouse flap structure specific endonuclease 1 (Fen1), 200ul, >10^7 TU/mL |
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MR220960L4V | Origene Technologies GmbH | 200 µl | Ask for price |
Lenti ORF particles, Fen1 (GFP-tagged ORF) - Rat flap structure-specific endonuclease 1 (Fen1), 200ul, >10^7 TU/mL |
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RR207787L4V | Origene Technologies GmbH | 200 µl | Ask for price |
1000 ug/mL Fenamidone in HPLC Acetonitrile - 1ML |
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LCS-4966 | Scientific Laboratory Supplies | 1ML | EUR 390.15 |
1000 ug/mL Fenazaquin in HPLC Acetonitrile - 1ML |
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LCS-6019 | Scientific Laboratory Supplies | 1ML | EUR 398.25 |
1000 ug/mL of Fenitrothion in Acetone 1mL Ampule - 1ML |
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S-2001 | Scientific Laboratory Supplies | 1ML | EUR 67.5 |
Lenti ORF particles, Fen1 (Myc-DDK-tagged) - Mouse flap structure specific endonuclease 1 (Fen1), 200ul, >10^7 TU/mL |
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MR220960L3V | Origene Technologies GmbH | 200 µl | Ask for price |
Lenti ORF particles, FEN1 (Myc-DDK tagged) - Human flap structure-specific endonuclease 1 (FEN1), 200ul, >10^7 TU/mL |
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RC201785L1V | Origene Technologies GmbH | 200 µl | Ask for price |
Lenti ORF particles, FEN1 (mGFP-tagged) - Human flap structure-specific endonuclease 1 (FEN1), 200ul, >10^7 TU/mL |
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RC201785L2V | Origene Technologies GmbH | 200 µl | Ask for price |
Lenti ORF particles, FEN1 (Myc-DDK tagged) - Human flap structure-specific endonuclease 1 (FEN1), 200ul, >10^7 TU/mL |
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RC201785L3V | Origene Technologies GmbH | 200 µl | Ask for price |
Lenti ORF particles, FEN1 (mGFP-tagged) - Human flap structure-specific endonuclease 1 (FEN1), 200ul, >10^7 TU/mL |
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RC201785L4V | Origene Technologies GmbH | 200 µl | Ask for price |
FEN1 Rabbit mAb |
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59492 | SAB | 100ul | EUR 339 |
FEN1 Rabbit mAb |
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52758 | SAB | 100ul | EUR 499 |
FEN1 Rabbit pAb |
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A1175-100ul | Abclonal | 100 ul | EUR 369.6 |
FEN1 Rabbit pAb |
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A1175-200ul | Abclonal | 200 ul | EUR 550.8 |
FEN1 Rabbit pAb |
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A1175-20ul | Abclonal | 20 ul | EUR 219.6 |
FEN1 Rabbit pAb |
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A1175-50ul | Abclonal | 50 ul | EUR 267.6 |
FEN-1 Rabbit mAb |
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A8999 | Abclonal | 20 μL | EUR 128.7 |
Description: A synthetic peptide corresponding to a sequence within amino acids 5000-5100 of human MUC2 (Q02817). |
FEN1 protein |
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30R-1442 | Fitzgerald | 100 ug | EUR 410 |
Description: Purified recombinant Human FEN1 protein |
FEN1 Rabbit pAb |
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E2381904 | EnoGene | 100ul | EUR 225 |
Description: Available in various conjugation types. |
FEN1 Rabbit mAb |
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E2R26747 | EnoGene | 100ul | EUR 255 |
Description: Available in various conjugation types. |
FEN1 Rabbit pAb |
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E2501175 | EnoGene | 100ul | EUR 225 |
Description: Available in various conjugation types. |
FEN1 Rabbit pAb |
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A0129-100ul | Abclonal | 100 ul | EUR 369.6 |
FEN1 Rabbit pAb |
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A0129-200ul | Abclonal | 200 ul | EUR 550.8 |
FEN1 Rabbit pAb |
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A0129-20ul | Abclonal | 20 ul | Ask for price |
FEN1 Rabbit pAb |
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A0129-50ul | Abclonal | 50 ul | Ask for price |
FEN1 Rabbit mAb |
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E2R381904 | EnoGene | 100ul | EUR 275 |
Description: Biotin-Conjugated, FITC-Conjugated , AF350 Conjugated , AF405M-Conjugated ,AF488-Conjugated, AF514-Conjugated ,AF532-Conjugated, AF555-Conjugated ,AF568-Conjugated , HRP-Conjugated, AF405S-Conjugated, AF405L-Conjugated , AF546-Conjugated, AF594-Conjugated , AF610-Conjugated, AF635-Conjugated , AF647-Conjugated , AF680-Conjugated , AF700-Conjugated , AF750-Conjugated , AF790-Conjugated , APC-Conjugated , PE-Conjugated , Cy3-Conjugated , Cy5-Conjugated , Cy5.5-Conjugated , Cy7-Conjugated Antibody |
1000 ug/mL (E)-Fenpyroximate in Acetonitrile - 1ML |
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S-6179 | Scientific Laboratory Supplies | 1ML | EUR 136.35 |
Fen1 (untagged ORF) - Rat flap structure-specific endonuclease 1 (Fen1), (10 ug) |
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RN207787 | Origene Technologies GmbH | 10 µg | Ask for price |
Lenti ORF particles, Fen1 (Myc-DDK-tagged ORF) - Rat flap structure-specific endonuclease 1 (Fen1), 200ul, >10^7 TU/mL |
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RR207787L3V | Origene Technologies GmbH | 200 µl | Ask for price |
Fen1 (GFP-tagged) - Mouse flap structure specific endonuclease 1 (Fen1), (10ug) |
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MG220960 | Origene Technologies GmbH | 10 µg | Ask for price |
Fen1 (untagged) - Mouse flap structure specific endonuclease 1 (Fen1), (10ug) |
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MC208478 | Origene Technologies GmbH | 10 µg | Ask for price |
FEN1 (GFP-tagged) - Human flap structure-specific endonuclease 1 (FEN1) |
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RG201785 | Origene Technologies GmbH | 10 µg | Ask for price |
FEN1 (untagged)-Human flap structure-specific endonuclease 1 (FEN1) |
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SC110892 | Origene Technologies GmbH | 10 µg | Ask for price |
FEN1 Antibody |
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ABD6309 | Lifescience Market | 100 ug | EUR 525.6 |
FEN1 Antibody |
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ABD13091 | Lifescience Market | 100 ug | EUR 525.6 |
FEN1 Antibody |
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8C10585 | AAT Bioquest | 50ug | EUR 368 |
Description: FEN1 Antibody |
FEN1 antibody |
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10R-1738 | Fitzgerald | 100 ug | EUR 614.4 |
Description: Mouse monoclonal FEN1 antibody |
FEN1 Antibody |
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32204 | SAB | 100ul | EUR 439 |
FEN1 Antibody |
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32204-100ul | SAB | 100ul | EUR 302.4 |
FEN1 Antibody |
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33763 | SAB | 100ul | EUR 319 |
FEN1 Antibody |
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33763-100ul | SAB | 100ul | EUR 302.4 |
FEN1 Antibody |
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33763-50ul | SAB | 50ul | EUR 224.4 |
FEN1 Antibody |
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CSB-PA926648- | Cusabio | each | EUR 402 |
Description: A polyclonal antibody against FEN1. Recognizes FEN1 from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, WB, IHC, IF;WB:1:500-1:3000, IHC:1:50-1:100, IF:1:100-1:500 |
FEN1 Antibody |
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CSB-PA926648-100ul | Cusabio | 100ul | EUR 379.2 |
Description: A polyclonal antibody against FEN1. Recognizes FEN1 from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, WB, IHC, IF;WB:1:500-1:3000, IHC:1:50-1:100, IF:1:100-1:500 |
FEN1 Antibody |
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E033763 | EnoGene | 100μg/100μl | EUR 255 |
Description: Available in various conjugation types. |
FEN1 Antibody |
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E11-10585C | EnoGene | 100μg | EUR 225 |
Description: Available in various conjugation types. |
FEN1 Antibody |
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E19-3172 | EnoGene | 100μg/100μl | EUR 225 |
Description: Available in various conjugation types. |
FEN1 Antibody |
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E19-6309 | EnoGene | 100μg/100μl | EUR 225 |
Description: Available in various conjugation types. |
FEN1 Antibody |
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DF6309 | Affbiotech | 200ul | EUR 420 |
FEN1 Antibody |
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DF6309-100ul | Affinity Biosciences | 100ul | EUR 280 |
FEN1 Antibody |
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DF6309-200ul | Affinity Biosciences | 200ul | EUR 350 |
FEN1 Antibody |
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DF3172 | Affbiotech | 200ul | EUR 420 |
FEN1 Antibody |
|||
E91175 | EnoGene | 100ul | EUR 255 |
Description: Available in various conjugation types. |
FEN1 Antibody |
|||
E38PA1417 | EnoGene | 100ul | EUR 225 |
Description: Available in various conjugation types. |
FEN1 Antibody |
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E309179 | EnoGene | 200ul | EUR 275 |
Description: Available in various conjugation types. |
FEN1 Antibody |
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CAC11682-100ul | Biomatik Corporation | 100ul | EUR 314 |
FEN1 Antibody |
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CAC11682-50ul | Biomatik Corporation | 50ul | EUR 199.2 |
FEN1 antibody |
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70R-1605 | Fitzgerald | 100 ug | EUR 407 |
Description: Rabbit polyclonal FEN1 antibody raised against the C terminal of FEN1 |
FEN1 antibody |
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70R-17281 | Fitzgerald | 50 ul | EUR 289 |
Description: Rabbit polyclonal FEN1 antibody |
FEN1 antibody |
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70R-31706 | Fitzgerald | 100 ug | EUR 294 |
Description: Rabbit polyclonal FEN1 antibody |
FEN1 antibody |
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70R-49736 | Fitzgerald | 100 ul | EUR 242 |
Description: Purified Polyclonal FEN1 antibody |
FEN1 Antibody |
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1-CSB-PA008585GA01HU | Cusabio |
|
|
Description: A polyclonal antibody against FEN1. Recognizes FEN1 from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, WB |
FEN1 Antibody |
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CSB-PA008585KA01HU- | Cusabio | each | EUR 402 |
Description: A polyclonal antibody against FEN1. Recognizes FEN1 from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, WB, IHC;WB:1:500-1:2000, IHC:1:50-1:200 |
FEN1 Antibody |
|||
CSB-PA008585KA01HU-100ul | Cusabio | 100ul | EUR 466.8 |
Description: A polyclonal antibody against FEN1. Recognizes FEN1 from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, WB, IHC;WB:1:500-1:2000, IHC:1:50-1:200 |
FEN1 Antibody |
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ABD3172 | Lifescience Market | 100 ug | EUR 525.6 |
FEN1 Antibody |
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1-CSB-PA002510 | Cusabio |
|
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Description: A polyclonal antibody against FEN1. Recognizes FEN1 from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: WB, IHC, IF, ELISA;WB:1/500-1/2000.IHC:1/100-1/300.IF:1/200-1/1000.ELISA:1/20000 |
FEN1 Antibody |
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GWB-5BB6C0 | GenWay Biotech | 0.1 mg | Ask for price |
FEN1 Antibody |
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GWB-8C5945 | GenWay Biotech | 0.1 mg | Ask for price |
FEN1 Antibody |
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GWB-670BF0 | GenWay Biotech | 0.1 ml | Ask for price |
FEN1 Antibody |
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GWB-MQ320E | GenWay Biotech | 50ug | Ask for price |
FEN1 Antibody |
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GWB-MQ321F | GenWay Biotech | 100ug | Ask for price |
FEN-1 Antibody |
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RQ5695 | NSJ Bioreagents | 100 ug | EUR 356.15 |
Description: Flap endonuclease 1 is an enzyme that in humans is encoded by the FEN1 gene. It is mapped to 11q12.2. The protein encoded by this gene removes 5' overhanging flaps in DNA repair and processes the 5' ends of Okazaki fragments in lagging strand DNA synthesis. Direct physical interaction between this protein and AP endonuclease 1 during long-patch base excision repair provides coordinated loading of the proteins onto the substrate, thus passing the substrate from one enzyme to another. The protein is a member of the XPG/RAD2 endonuclease family and is one of ten proteins essential for cell-free DNA replication. DNA secondary structure can inhibit flap processing at certain trinucleotide repeats in a length-dependent manner by concealing the 5' end of the flap that is necessary for both binding and cleavage by the protein encoded by this gene. Therefore, secondary structure can deter the protective function of this protein, leading to site-specific trinucleotide expansions. |
FEN1-IN-SC13 |
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T9743-10mg | TargetMol Chemicals | 10mg | Ask for price |
Description: FEN1-IN-SC13 |
FEN1-IN-SC13 |
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T9743-1g | TargetMol Chemicals | 1g | Ask for price |
Description: FEN1-IN-SC13 |
FEN1-IN-SC13 |
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T9743-1mg | TargetMol Chemicals | 1mg | Ask for price |
Description: FEN1-IN-SC13 |
FEN1-IN-SC13 |
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T9743-50mg | TargetMol Chemicals | 50mg | Ask for price |
Description: FEN1-IN-SC13 |
FEN1-IN-SC13 |
|||
T9743-5mg | TargetMol Chemicals | 5mg | Ask for price |
Description: FEN1-IN-SC13 |
FEN1 Antibody |
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abx243080-96tests | Abbexa | 96 tests | EUR 287.5 |
1000 ug/mL (E)-Fenpyroximate in HPLC Acetonitrile - 1ML |
|||
LCS-6179 | Scientific Laboratory Supplies | 1ML | EUR 398.25 |
1000 ug/mL Fenpyroximate in HPLC Acetonitrile - 1ML |
|||
LCS-6387 | Scientific Laboratory Supplies | 1ML | EUR 218.7 |
1000 ug/mL Fenthion sulfoxide in Acetonitrile - 1ML |
|||
S-4316-ACN | Scientific Laboratory Supplies | 1ML | EUR 125.55 |
Fen1 (Myc-DDK-tagged) - Mouse flap structure specific endonuclease 1 (Fen1) |
|||
MR220960 | Origene Technologies GmbH | 10 µg | Ask for price |
Fen1 (Myc-DDK-tagged ORF) - Rat flap structure-specific endonuclease 1 (Fen1), (10 ug) |
|||
RR207787 | Origene Technologies GmbH | 10 µg | Ask for price |
FEN1 ELISA KIT|Human |
|||
EF009610 | Lifescience Market | 96 Tests | EUR 826.8 |
FEN1 (Myc-DDK-tagged)-Human flap structure-specific endonuclease 1 (FEN1) |
|||
RC201785 | Origene Technologies GmbH | 10 µg | Ask for price |
1000 ug/mL Fenthion-sulfone in HPLC Acetonitrile - 1ML |
|||
LCS-6063 | Scientific Laboratory Supplies | 1ML | EUR 330.75 |
anti- FEN1 antibody |
|||
FNab03075 | FN Test | 100µg | EUR 606.3 |
Description: Antibody raised against FEN1 |
Fen1 ORF Vector (Rat) (pORF) |
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ORF067064 | ABM | 1.0 ug DNA | EUR 607.2 |
Lenti ORF clone of Fen1 (mGFP-tagged) - Mouse flap structure specific endonuclease 1 (Fen1) |
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MR220960L4 | Origene Technologies GmbH | 10 µg | Ask for price |
FEN1 (Center) Rabbit pAb |
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E2614818 | EnoGene | 100ul | EUR 225 |
Description: Available in various conjugation types. |
Rat FEN1 shRNA Plasmid |
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20-abx986959 | Abbexa |
|
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1000 ug/mL Fensulfothion-oxon in HPLC Acetonitrile - 1ML |
|||
LCS-6209 | Scientific Laboratory Supplies | 1ML | EUR 724.95 |
1000 ug/mL Fenhexamid in Acetonitrile Volume: 1mL Ampule - 1ML |
|||
S-4962-ACN | Scientific Laboratory Supplies | 1ML | EUR 130.95 |
1000 ug/mL Fenamidone in Acetonitrile Volume: 1mL Ampule - 1ML |
|||
S-4966-ACN | Scientific Laboratory Supplies | 1ML | EUR 136.35 |
Lenti ORF clone of Fen1 (Myc-DDK-tagged) - Mouse flap structure specific endonuclease 1 (Fen1) |
|||
MR220960L3 | Origene Technologies GmbH | 10 µg | Ask for price |
Lenti ORF clone of Fen1 (mGFP-tagged ORF) - Rat flap structure-specific endonuclease 1 (Fen1), (10 ug) |
|||
RR207787L4 | Origene Technologies GmbH | 10 µg | Ask for price |
FEN1 cloning plasmid |
|||
CSB-CL008585HU-10ug | Cusabio | 10ug | EUR 279.6 |
Description: A cloning plasmid for the FEN1 gene. |
Fen1 ORF Vector (Mouse) (pORF) |
|||
ORF044773 | ABM | 1.0 ug DNA | EUR 607.2 |
FEN1 ORF Vector (Human) (pORF) |
|||
ORF004018 | ABM | 1.0 ug DNA | EUR 114 |
FEN1 3'UTR GFP Stable Cell Line |
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TU057883 | ABM | 1.0 ml | EUR 1672.8 |
Fen1 3'UTR GFP Stable Cell Line |
|||
TU254569 | ABM | 1.0 ml | Ask for price |
Fen1 3'UTR GFP Stable Cell Line |
|||
TU156502 | ABM | 1.0 ml | Ask for price |
Fen1 (untagged) - Mouse flap structure specific endonuclease 1 (Fen1), transcript variant 2 |
|||
MC227399 | Origene Technologies GmbH | 10 µg | Ask for price |
Fen1 (untagged) - Mouse flap structure specific endonuclease 1 (Fen1), transcript variant 3 |
|||
MC227400 | Origene Technologies GmbH | 10 µg | Ask for price |
Lenti ORF clone of Fen1 (Myc-DDK-tagged ORF) - Rat flap structure-specific endonuclease 1 (Fen1), (10 ug) |
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RR207787L3 | Origene Technologies GmbH | 10 µg | Ask for price |
FEN1 Blocking Peptide |
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20-abx062611 | Abbexa |
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FEN1 Blocking Peptide |
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33R-7232 | Fitzgerald | 100 ug | EUR 119 |
Description: A synthetic peptide for use as a blocking control in assays to test for specificity of FEN1 antibody, catalog no. 70R-1605 |
Rad2 / FEN1 (pS187) Antibody |
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abx215327-100ug | Abbexa | 100 ug | EUR 526.8 |
FEN1 Blocking Peptide |
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DF6309-BP | Affbiotech | 1mg | EUR 234 |
FEN1 Blocking Peptide |
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DF3172-BP | Affbiotech | 1mg | EUR 234 |
Human FEN1 shRNA Plasmid |
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20-abx951559 | Abbexa |
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FEN1P1 ORF Vector (Human) (pORF) |
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ORF019564 | ABM | 1.0 ug DNA | Ask for price |
Fen1 (myc-DDK-tagged) - Mouse flap structure specific endonuclease 1 (Fen1), transcript variant 2 |
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MR229610 | Origene Technologies GmbH | 10 µg | Ask for price |
Fen1 (myc-DDK-tagged) - Mouse flap structure specific endonuclease 1 (Fen1), transcript variant 3 |
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MR229611 | Origene Technologies GmbH | 10 µg | Ask for price |
1000 ug/mL Fenbutatin-oxide in LCMS Grade AcetoneVolume: 1mL Ampule - 1ML |
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LCS-1999-AC | Scientific Laboratory Supplies | 1ML | EUR 244.35 |
1000 ug/mL Fensulfothion-oxon-sulfone in HPLC Acetonitrile - 1ML |
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LCS-6210 | Scientific Laboratory Supplies | 1ML | EUR 414.45 |
FEN-1 Polyclonal Antibody |
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ABP51337-003ml | Abbkine | 0.03ml | EUR 189.6 |
Description: A polyclonal antibody for detection of FEN-1 from Human, Mouse, Rat. This FEN-1 antibody is for WB, IHC-P, IF, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from the Internal region of human FEN-1 at AA range: 60-140 |
FEN-1 Polyclonal Antibody |
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ABP51337-01ml | Abbkine | 0.1ml | EUR 346.8 |
Description: A polyclonal antibody for detection of FEN-1 from Human, Mouse, Rat. This FEN-1 antibody is for WB, IHC-P, IF, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from the Internal region of human FEN-1 at AA range: 60-140 |
FEN-1 Polyclonal Antibody |
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ABP51337-02ml | Abbkine | 0.2ml | EUR 496.8 |
Description: A polyclonal antibody for detection of FEN-1 from Human, Mouse, Rat. This FEN-1 antibody is for WB, IHC-P, IF, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from the Internal region of human FEN-1 at AA range: 60-140 |
FEN-1 Polyclonal Antibody |
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40922-100ul | SAB | 100ul | EUR 302.4 |
FEN-1 Polyclonal Antibody |
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40922-50ul | SAB | 50ul | EUR 224.4 |
FEN-1 Polyclonal Antibody |
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E040922 | EnoGene | 100μg/100μl | EUR 255 |
Description: Available in various conjugation types. |
FEN-1 Polyclonal Antibody |
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E20-71689 | EnoGene | 100ug | EUR 225 |
Description: Available in various conjugation types. |
FEN1 Conjugated Antibody |
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C32204 | SAB | 100ul | EUR 476.4 |
Human FEN1 Protein Lysate 20ug |
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IHUFEN1PLLY20UG | Innovative research | each | EUR 213 |
Description: Human FEN1 Protein Lysate 20ug |
FEN1 ELISA Kit (Human) (OKEH08213) |
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OKEH08213 | Aviva Systems Biology | 96 Wells | EUR 1075.2 |
Description: Description of target: The protein encoded by this gene removes 5' overhanging flaps in DNA repair and processes the 5' ends of Okazaki fragments in lagging strand DNA synthesis. Direct physical interaction between this protein and AP endonuclease 1 during long-patch base excision repair provides coordinated loading of the proteins onto the substrate, thus passing the substrate from one enzyme to another. The protein is a member of the XPG/RAD2 endonuclease family and is one of ten proteins essential for cell-free DNA replication. DNA secondary structure can inhibit flap processing at certain trinucleotide repeats in a length-dependent manner by concealing the 5' end of the flap that is necessary for both binding and cleavage by the protein encoded by this gene. Therefore, secondary structure can deter the protective function of this protein, leading to site-specific trinucleotide expansions.;Species reactivity: Human;Application: ELISA;Assay info: Assay Methodology: Quantitative Competitive ELISA;Sensitivity: 0.136ng/mL |
Fen1 ELISA Kit (Mouse) (OKEH08214) |
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OKEH08214 | Aviva Systems Biology | 96 Wells | EUR 1075.2 |
Description: Description of target: ;Species reactivity: Mouse;Application: ELISA;Assay info: Assay Methodology: Quantitative Sandwich ELISA;Sensitivity: 0.27ng/mL |
FEN1 Protein Vector (Rat) (pPM-C-HA) |
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PV268256 | ABM | 500 ng | EUR 723.6 |
FEN-1 Polyclonal Antibody |
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E44H04707 | EnoGene | 100ul | EUR 255 |
Description: Biotin-Conjugated, FITC-Conjugated , AF350 Conjugated , AF405M-Conjugated ,AF488-Conjugated, AF514-Conjugated ,AF532-Conjugated, AF555-Conjugated ,AF568-Conjugated , HRP-Conjugated, AF405S-Conjugated, AF405L-Conjugated , AF546-Conjugated, AF594-Conjugated , AF610-Conjugated, AF635-Conjugated , AF647-Conjugated , AF680-Conjugated , AF700-Conjugated , AF750-Conjugated , AF790-Conjugated , APC-Conjugated , PE-Conjugated , Cy3-Conjugated , Cy5-Conjugated , Cy5.5-Conjugated , Cy7-Conjugated Antibody |
OPPA02024-5UG - FEN1 Protein |
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OPPA02024-5UG | Aviva Systems Biology | 5ug | EUR 70 |
FEN1 Protein Vector (Rat) (pPB-C-His) |
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PV268254 | ABM | 500 ng | EUR 723.6 |
FEN1 Protein Vector (Rat) (pPB-N-His) |
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PV268255 | ABM | 500 ng | EUR 723.6 |
FEN1 Protein Vector (Rat) (pPM-C-His) |
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PV268257 | ABM | 500 ng | EUR 723.6 |
FEN1 Recombinant Protein (Rat) |
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RP201188 | ABM | 100 ug | Ask for price |
FEN1 (A119) polyclonal antibody |
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E43S1989 | EnoGene | 100ul | EUR 225 |
Description: Available in various conjugation types. |
AAP46097-100UG - FEN1 Peptide |
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AAP46097-100UG | Aviva Systems Biology | 100ug | EUR 99 |
FEN1 Protein Vector (Human) (pPM-C-HA) |
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PV016071 | ABM | 500 ng | EUR 394.8 |
FEN1 Protein Vector (Mouse) (pPM-C-HA) |
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PV179092 | ABM | 500 ng | EUR 723.6 |
Rad2/FEN1 (Phospho- Ser187) Antibody |
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ABF8505 | Lifescience Market | 100 ug | EUR 525.6 |
OAAN00260-50UL - FEN1 Antibody |
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OAAN00260-50UL | Aviva Systems Biology | 50ul | EUR 149 |
FEN1 Protein Vector (Human) (pPB-C-His) |
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PV016069 | ABM | 500 ng | EUR 394.8 |
FEN1 Protein Vector (Human) (pPB-N-His) |
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PV016070 | ABM | 500 ng | EUR 394.8 |
FEN1 Protein Vector (Human) (pPM-C-His) |
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PV016072 | ABM | 500 ng | EUR 394.8 |
Prokaryotic expression of PE8 protein from Mycobacterium tuberculosis (H37Rv) and preparation of its polyclonal antibody in rabbits
Goal To clone proline-glutamate 8 (PE8) gene phase from Mycobacterium tuberculosis (H37Rv), assemble the recombinant plasmid pET28a-PE8, categorical recombinant PE8 protein, and put together its polyclonal antibody. Strategies Utilizing a regular homologous recombination cloning expertise, we cloned the PE8 gene into the prokaryotic vector pET28a. After sequence affirmation, it was reworked into E. coli BL21 (DE3) and handled with 0.5 mmol/L isopropyl-beta-D-thiogalactopyranoside (IPTG) to induce protein expression.
We purified and renatured the recombinant PE8 protein, and immunized New Zealand rabbits to arrange the polyclonal antibody. Antibody titer was decided by oblique ELISA and the specificity was evaluated by Western blot evaluation. Outcomes The recombinant plasmid pET28a-PE8 was efficiently constructed, and the PE8 protein was primarily expressed in an inclusion physique in E. coli. After renaturation and purification, a purity of about 90% of the recombinant protein was achieved.
The titer of the polyclonal antibody was greater than 1:430 080. The polyclonal antibody may particularly acknowledge the recombinant PE8 protein. Conclusion We have now efficiently expressed and purified recombinant PE8 protein, which might be additional utilized to generate PE8 polyclonal antibody with acceptable titer and specificity.
A preliminary analysis of a regionally produced biotinylated polyclonal anti-rabies antibody for direct speedy immunohistochemical check (DRIT) within the Philippines
- Rabies is a deadly zoonotic illness endemic in creating nations of Asia and Africa. Lately, the direct speedy immunohistochemical check (DRIT) was advisable by the World Well being Group (WHO) and the World Group for Animal Well being (OIE) as a diagnostic check for rabies. Subsequently, a biotinylated polyclonal antibody (pAb) in opposition to the rabies lyssavirus (RABV) nucleoprotein was developed utilizing a plasmid cDNA vaccine derived from a problem virus normal 11 pressure.
- A preliminary analysis on the efficacy of this reagent in recognizing the Philippine RABV pressure was examined utilizing banked canine hippocampal tissue samples with DRIT and the outcomes have been in comparison with dFAT. The consequences of acetone and formalin fixation on DRIT have been additionally assessed by way of immunoreactivity scores of the specimens.
- Of the 142 samples examined, 104 examined constructive and 38 detrimental utilizing each dFAT and DRIT, displaying 100% settlement between the 2 diagnostic procedures. Furthermore, no false constructive or false detrimental outcomes have been noticed utilizing acetone and formalin fixation. Thus, regionally ready biotinylated pAb from plasmid cDNA can be utilized for DRIT, particularly in resource-limited laboratories within the Philippines.
- Nonetheless, these outcomes ought to be confirmed with a extra thorough analysis of this method, and the vary of detection must be additional evaluated in a bigger panel of animal samples and on different lyssaviruses.
Acetylcholinesterase (AChE) polyclonal antibody from hybrid catfish (C. macrocephalus × C. gariepinus): Specification, sensitivity and cross reactivity
AChE (acetylcholinesterase) is usually categorized as a selected biomarker of pesticide publicity. The goal of this examine was to supply AChE polyclonal antibody from hybrid catfish that have been uncovered to business glyphosate. The hybrid catfish was uncovered to glyphosate (0.75 mL/L) for 24 h. After that, the fish mind was dissected, AChE was extracted and purified by hydroxyapatite column chromatography and eluted with 0.2 M potassium phosphate buffer pH 6.8. This protocol gave 70% yield.
Then, the mind extract was characterised utilizing 10% SDS-PAGE and Western blot probed with business polyclonal antibody particular to AChE (PAb-AChE). The protein, 71 kDa, was then used as an antigen to immunize mice for antibody manufacturing.
The polyclonal antibody (PAb) was characterised utilizing dot blot, Western blot and immunohistochemistry for immunolocalization of AChE in hybrid catfish uncovered to glyphosate. We discovered that the suitable dilution of antibody for each dot blot and Western blot was 1:3500, and 1:2500 for immunohistochemistry.
Cross reactivity testing confirmed that PAb-AChE can be utilized with AChE from striped snakehead fish on the identical dilution as used with AChE from hybrid catfish. It was concluded that PAb particular to hybrid catfish AChE from this work was extremely particular and delicate, and might cross-react with striped snakehead fish AChE. Thus, this polyclonal antibody could also be utilized in monitoring glyphosate publicity in hybrid catfish and striped snakehead fish.