Prokaryotic expression of PE8 protein from Mycobacterium tuberculosis

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Improvement of a Extremely Delicate Enzyme-Linked Immunosorbent Assay for Mouse Soluble Epoxide Hydrolase Detection by Combining a Polyclonal Seize Antibody with a Nanobody Tracer

 

Enzyme-linked immunosorbent assays (ELISA) for the detection of soluble epoxide hydrolase (sEH), a key enzyme within the metabolism of fatty acids and a biomarker, might more and more signify an essential diagnostic software. Nonetheless, there’s a lack of ELISAs for mouse sEH quantification, thus leading to a bottleneck in understanding the pathogenesis of many ailments associated to sEH based mostly on mouse fashions.

On this work, nanobodies recognizing mouse sEH have been obtained by way of rebiopanning in opposition to mouse sEH within the earlier phage show library of human sEH. Later, we developed 4 ELISAs involving a mix of anti-mouse sEH polyclonal antibodies (pAbs) and nanobodies. It was discovered that the double antibodies labored as twin filters and had a big impact on each the sensitivity and selectivity of sandwich immunoassays.

The change from anti-human sEH pAbs to anti-mouse sEH pAbs led to over a 100-fold improve within the sensitivity and a dramatic lower of the restrict of detection to a picogram per milliliter vary in format B (pAb/biotin-VHH/streptavidin-poly-horseradish peroxidase). Furthermore, we discovered that the 4 sandwich ELISAs would possibly exhibit glorious selectivities to mouse sEH, regardless of the antibodies alone displaying important cross-reactivity to the matrix, indicating the improved selectivity of double antibodies as twin filters.

Finally, for the primary time, the ELISA (format B) was efficiently used to measure the mouse sEH stage in most cancers cells with ultralow abundances. The ELISAs proposed right here signify a delicate software for monitoring sEH in numerous organic processes and likewise present deep insights into creating sandwich immunoassays in opposition to numerous targets when it comes to each the sensitivity and selectivity.

Prediction of Clearance of Monoclonal and Polyclonal Antibodies and Non-Antibody Proteins in Kids: Software of Allometric Scaling

 

Allometric scaling can be utilized for the extrapolation of pharmacokinetic parameters from adults to kids. The target of this examine was to foretell clearance of therapeutic proteins (monoclonal and polyclonal antibodies and non-antibody proteins) allometrically in preterm neonates to adolescents. There have been 13 monoclonal antibodies, seven polyclonal antibodies, and 9 therapeutic proteins (non-antibodies) within the examine.

The clearance of therapeutic proteins was predicted utilizing the age dependent exponents (ADE) mannequin after which in contrast with the noticed clearance values. There have been in complete 29 therapeutic proteins on this examine with 75 observations. The variety of observations with ≤30%, ≤50%, and >50% prediction error was 60 (80%), 72 (96%), and three (4%), respectively.

General, the anticipated clearance values of therapeutic proteins in kids was good. The allometric methodology proposed on this manuscript can be utilized to pick first-in-pediatric dose of therapeutic proteins in pediatric medical trials.

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Fenretinide

GP8861-25MG 25 mg
EUR 170.4

Fenretinide

HY-15373 10mM/1mL
EUR 151.2

Fenretinide

GP8861-10 10
EUR 63.3

Fenretinide

GP8861-25 25
EUR 110.7

Fen1/ Rat Fen1 ELISA Kit

ELI-27513r 96 Tests
EUR 1063.2

1000 ug/mL Fenthion in Acetone

S-2010-AC 1ML
EUR 168

1000 ug/mL Fenobucarb in Acetone

S-4610-AC 1ML
EUR 94.8

1000 ug/mL Fenpyroximate in Acetone

S-5804 1ML
EUR 230.4

FEN1 siRNA

20-abx916746
  • EUR 661.20
  • EUR 878.40
  • 15 nmol
  • 30 nmol

FEN1 siRNA

20-abx916747
  • EUR 661.20
  • EUR 878.40
  • 15 nmol
  • 30 nmol

1000 ug/mL Fenamidone in HPLC Acetonitrile

LCS-4966 1ML
EUR 346.8

1000 ug/mL Fenazaquin in HPLC Acetonitrile

LCS-6019 1ML
EUR 354

1000 ug/mL of Fenitrothion in Acetone 1mL Ampule

S-2001 1ML
EUR 60

FEN1 Rabbit pAb

A0129-100ul 100 ul
EUR 369.6

FEN1 Rabbit pAb

A0129-200ul 200 ul
EUR 550.8

FEN1 Rabbit pAb

A0129-20ul 20 ul Ask for price

FEN1 Rabbit pAb

A0129-50ul 50 ul Ask for price

FEN1 Rabbit pAb

A1175-100ul 100 ul
EUR 369.6

FEN1 Rabbit pAb

A1175-200ul 200 ul
EUR 550.8

FEN1 Rabbit pAb

A1175-20ul 20 ul
EUR 219.6

FEN1 Rabbit pAb

A1175-50ul 50 ul
EUR 267.6

FEN1 Rabbit mAb

A8999 20 μL
EUR 18

FEN1 protein

30R-1442 100 ug
EUR 476.4
Description: Purified recombinant Human FEN1 protein

1000 ug/mL (E)-Fenpyroximate in Acetonitrile

S-6179 1ML
EUR 121.2

FEN1 antibody

70R-17281 50 ul
EUR 522
Description: Rabbit polyclonal FEN1 antibody

FEN1 antibody

70R-1605 100 ug
EUR 452.4
Description: Rabbit polyclonal FEN1 antibody raised against the C terminal of FEN1

FEN1 antibody

10R-1738 100 ug
EUR 614.4
Description: Mouse monoclonal FEN1 antibody

FEN1 antibody

70R-49736 100 ul
EUR 292.8
Description: Purified Polyclonal FEN1 antibody

FEN1 antibody

70R-31706 100 ug
EUR 392.4
Description: Rabbit polyclonal FEN1 antibody

FEN1 Antibody

ABD13091 100 ug
EUR 525.6

FEN1 Antibody

ABD3172 100 ug
EUR 525.6

FEN1 Antibody

ABD6309 100 ug
EUR 525.6

FEN1 Antibody

32204-100ul 100ul
EUR 302.4

FEN1 Antibody

33763-100ul 100ul
EUR 302.4

FEN1 Antibody

33763-50ul 50ul
EUR 224.4

FEN1 Antibody

1-CSB-PA002510
  • EUR 266.40
  • EUR 234.00
  • 100ug
  • 50ug
Description: A polyclonal antibody against FEN1. Recognizes FEN1 from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: WB, IHC, IF, ELISA;WB:1/500-1/2000.IHC:1/100-1/300.IF:1/200-1/1000.ELISA:1/20000

FEN1 Antibody

CSB-PA008585KA01HU- each
EUR 402
Description: A polyclonal antibody against FEN1. Recognizes FEN1 from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, WB, IHC;WB:1:500-1:2000, IHC:1:50-1:200

FEN1 Antibody

CSB-PA008585KA01HU-100ul 100ul
EUR 466.8
Description: A polyclonal antibody against FEN1. Recognizes FEN1 from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, WB, IHC;WB:1:500-1:2000, IHC:1:50-1:200

FEN1 Antibody

1-CSB-PA008585GA01HU
  • EUR 716.40
  • EUR 399.60
  • 150ul
  • 50ul
Description: A polyclonal antibody against FEN1. Recognizes FEN1 from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, WB

FEN1 Antibody

CSB-PA926648- each
EUR 402
Description: A polyclonal antibody against FEN1. Recognizes FEN1 from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, WB, IHC, IF;WB:1:500-1:3000, IHC:1:50-1:100, IF:1:100-1:500

FEN1 Antibody

CSB-PA926648-100ul 100ul
EUR 379.2
Description: A polyclonal antibody against FEN1. Recognizes FEN1 from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, WB, IHC, IF;WB:1:500-1:3000, IHC:1:50-1:100, IF:1:100-1:500

FEN1 Antibody

DF6309 200ul
EUR 420

FEN1 Antibody

DF3172 200ul
EUR 420

FEN-1 Antibody

RQ5695 100 ug
EUR 356.15
Description: Flap endonuclease 1 is an enzyme that in humans is encoded by the FEN1 gene. It is mapped to 11q12.2. The protein encoded by this gene removes 5' overhanging flaps in DNA repair and processes the 5' ends of Okazaki fragments in lagging strand DNA synthesis. Direct physical interaction between this protein and AP endonuclease 1 during long-patch base excision repair provides coordinated loading of the proteins onto the substrate, thus passing the substrate from one enzyme to another. The protein is a member of the XPG/RAD2 endonuclease family and is one of ten proteins essential for cell-free DNA replication. DNA secondary structure can inhibit flap processing at certain trinucleotide repeats in a length-dependent manner by concealing the 5' end of the flap that is necessary for both binding and cleavage by the protein encoded by this gene. Therefore, secondary structure can deter the protective function of this protein, leading to site-specific trinucleotide expansions.

1000 ug/mL (E)-Fenpyroximate in HPLC Acetonitrile

LCS-6179 1ML
EUR 354

1000 ug/mL Fenpyroximate in HPLC Acetonitrile

LCS-6387 1ML
EUR 194.4

1000 ug/mL Fenthion sulfoxide in Acetonitrile

S-4316-ACN 1ML
EUR 111.6

FEN1 ELISA KIT|Human

EF009610 96 Tests
EUR 826.8

1000 ug/mL Fenthion-sulfone in HPLC Acetonitrile

LCS-6063 1ML
EUR 294

anti- FEN1 antibody

FNab03075 100µg
EUR 606.3
Description: Antibody raised against FEN1

Fen1 ORF Vector (Rat) (pORF)

ORF067064 1.0 ug DNA
EUR 607.2

Rat FEN1 shRNA Plasmid

20-abx986959
  • EUR 961.20
  • EUR 1345.20
  • 150 µg
  • 300 µg

1000 ug/mL Fensulfothion-oxon in HPLC Acetonitrile

LCS-6209 1ML
EUR 644.4

1000 ug/mL Fenhexamid in Acetonitrile Volume: 1mL Ampule

S-4962-ACN 1ML
EUR 116.4

1000 ug/mL Fenamidone in Acetonitrile Volume: 1mL Ampule

S-4966-ACN 1ML
EUR 121.2

FEN1 cloning plasmid

CSB-CL008585HU-10ug 10ug
EUR 279.6
Description: A cloning plasmid for the FEN1 gene.

FEN1 ORF Vector (Human) (pORF)

ORF004018 1.0 ug DNA
EUR 114

Fen1 ORF Vector (Mouse) (pORF)

ORF044773 1.0 ug DNA
EUR 607.2

FEN1 3'UTR GFP Stable Cell Line

TU057883 1.0 ml
EUR 1672.8

Fen1 3'UTR GFP Stable Cell Line

TU254569 1.0 ml Ask for price

Fen1 3'UTR GFP Stable Cell Line

TU156502 1.0 ml Ask for price

Rad2 / FEN1 (pS187) Antibody

abx215327-100ug 100 ug
EUR 526.8

FEN1 Blocking Peptide

33R-7232 100 ug
EUR 216
Description: A synthetic peptide for use as a blocking control in assays to test for specificity of FEN1 antibody, catalog no. 70R-1605

FEN1 Blocking Peptide

20-abx062611
  • EUR 326.40
  • EUR 493.20
  • 1 mg
  • 5 mg

Human FEN1 shRNA Plasmid

20-abx951559
  • EUR 961.20
  • EUR 1345.20
  • 150 µg
  • 300 µg

FEN1 Blocking Peptide

DF6309-BP 1mg
EUR 234

FEN1 Blocking Peptide

DF3172-BP 1mg
EUR 234

FEN1P1 ORF Vector (Human) (pORF)

ORF019564 1.0 ug DNA Ask for price

1000 ug/mL Fenbutatin-oxide in LCMS Grade AcetoneVolume: 1mL Ampule

LCS-1999-AC 1ML
EUR 217.2

1000 ug/mL Fensulfothion-oxon-sulfone in HPLC Acetonitrile

LCS-6210 1ML
EUR 368.4

FEN-1 Polyclonal Antibody

40922-100ul 100ul
EUR 302.4

FEN-1 Polyclonal Antibody

ABP51337-003ml 0.03ml
EUR 189.6
Description: A polyclonal antibody for detection of FEN-1 from Human, Mouse, Rat. This FEN-1 antibody is for WB, IHC-P, IF, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from the Internal region of human FEN-1 at AA range: 60-140

FEN-1 Polyclonal Antibody

ABP51337-01ml 0.1ml
EUR 346.8
Description: A polyclonal antibody for detection of FEN-1 from Human, Mouse, Rat. This FEN-1 antibody is for WB, IHC-P, IF, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from the Internal region of human FEN-1 at AA range: 60-140

FEN-1 Polyclonal Antibody

ABP51337-02ml 0.2ml
EUR 496.8
Description: A polyclonal antibody for detection of FEN-1 from Human, Mouse, Rat. This FEN-1 antibody is for WB, IHC-P, IF, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from the Internal region of human FEN-1 at AA range: 60-140

FEN-1 Polyclonal Antibody

40922-50ul 50ul
EUR 224.4

FEN1 Conjugated Antibody

C32204 100ul
EUR 476.4

FEN1 ELISA Kit (Human) (OKEH08213)

OKEH08213 96 Wells
EUR 1075.2
Description: Description of target: The protein encoded by this gene removes 5' overhanging flaps in DNA repair and processes the 5' ends of Okazaki fragments in lagging strand DNA synthesis. Direct physical interaction between this protein and AP endonuclease 1 during long-patch base excision repair provides coordinated loading of the proteins onto the substrate, thus passing the substrate from one enzyme to another. The protein is a member of the XPG/RAD2 endonuclease family and is one of ten proteins essential for cell-free DNA replication. DNA secondary structure can inhibit flap processing at certain trinucleotide repeats in a length-dependent manner by concealing the 5' end of the flap that is necessary for both binding and cleavage by the protein encoded by this gene. Therefore, secondary structure can deter the protective function of this protein, leading to site-specific trinucleotide expansions.;Species reactivity: Human;Application: ELISA;Assay info: Assay Methodology: Quantitative Competitive ELISA;Sensitivity: 0.136ng/mL

Fen1 ELISA Kit (Mouse) (OKEH08214)

OKEH08214 96 Wells
EUR 1075.2
Description: Description of target: ;Species reactivity: Mouse;Application: ELISA;Assay info: Assay Methodology: Quantitative Sandwich ELISA;Sensitivity: 0.27ng/mL

FEN-1 Polyclonal Antibody

ES2336-100ul 100ul
EUR 334.8
Description: A Rabbit Polyclonal antibody against FEN-1 from Human/Mouse/Rat. This antibody is tested and validated for WB, ELISA, IHC, IF, WB, ELISA

FEN-1 Polyclonal Antibody

ES2336-50ul 50ul
EUR 248.4
Description: A Rabbit Polyclonal antibody against FEN-1 from Human/Mouse/Rat. This antibody is tested and validated for WB, ELISA, IHC, IF, WB, ELISA

FEN1 Protein Vector (Rat) (pPM-C-HA)

PV268256 500 ng
EUR 723.6

FEN1 Recombinant Protein (Rat)

RP201188 100 ug Ask for price

FEN1 Protein Vector (Rat) (pPB-C-His)

PV268254 500 ng
EUR 723.6

FEN1 Protein Vector (Rat) (pPB-N-His)

PV268255 500 ng
EUR 723.6

FEN1 Protein Vector (Rat) (pPM-C-His)

PV268257 500 ng
EUR 723.6

FEN1 Protein Vector (Human) (pPM-C-HA)

PV016071 500 ng
EUR 394.8

FEN1 Protein Vector (Mouse) (pPM-C-HA)

PV179092 500 ng
EUR 723.6

Rad2/FEN1 (Phospho- Ser187) Antibody

ABF8505 100 ug
EUR 525.6

FEN1 Protein Vector (Human) (pPB-C-His)

PV016069 500 ng
EUR 394.8

FEN1 Protein Vector (Human) (pPB-N-His)

PV016070 500 ng
EUR 394.8

FEN1 Protein Vector (Human) (pPM-C-His)

PV016072 500 ng
EUR 394.8

FEN1 Protein Vector (Mouse) (pPB-C-His)

PV179090 500 ng
EUR 723.6

FEN1 Protein Vector (Mouse) (pPB-N-His)

PV179091 500 ng
EUR 723.6

FEN1 Protein Vector (Mouse) (pPM-C-His)

PV179093 500 ng
EUR 723.6

FEN1 Recombinant Protein (Human)

RP012052 100 ug Ask for price

FEN1 Recombinant Protein (Mouse)

RP134315 100 ug Ask for price

FEN1 3'UTR Luciferase Stable Cell Line

TU007883 1.0 ml
EUR 1672.8

Fen1 3'UTR Luciferase Stable Cell Line

TU106502 1.0 ml Ask for price

Fen1 3'UTR Luciferase Stable Cell Line

TU204569 1.0 ml Ask for price

Fen1 sgRNA CRISPR Lentivector set (Rat)

K7115801 3 x 1.0 ug
EUR 406.8

FEN1 Antibody / Flap endonuclease 1

RQ6987 100 ug
EUR 356.15
Description: Flap endonuclease 1 is an enzyme that in humans is encoded by the FEN1 gene. It is mapped to 11q12.2. The protein encoded by this gene removes 5' overhanging flaps in DNA repair and processes the 5' ends of Okazaki fragments in lagging strand DNA synthesis. Direct physical interaction between this protein and AP endonuclease 1 during long-patch base excision repair provides coordinated loading of the proteins onto the substrate, thus passing the substrate from one enzyme to another. The protein is a member of the XPG/RAD2 endonuclease family and is one of ten proteins essential for cell-free DNA replication. DNA secondary structure can inhibit flap processing at certain trinucleotide repeats in a length-dependent manner by concealing the 5' end of the flap that is necessary for both binding and cleavage by the protein encoded by this gene. Therefore, secondary structure can deter the protective function of this protein, leading to site-specific trinucleotide expansions.

FEN1 Antibody / Flap endonuclease 1

RQ6988 100 ug
EUR 356.15
Description: Flap endonuclease 1 is an enzyme that in humans is encoded by the FEN1 gene. It is mapped to 11q12.2. The protein encoded by this gene removes 5' overhanging flaps in DNA repair and processes the 5' ends of Okazaki fragments in lagging strand DNA synthesis. Direct physical interaction between this protein and AP endonuclease 1 during long-patch base excision repair provides coordinated loading of the proteins onto the substrate, thus passing the substrate from one enzyme to another. The protein is a member of the XPG/RAD2 endonuclease family and is one of ten proteins essential for cell-free DNA replication. DNA secondary structure can inhibit flap processing at certain trinucleotide repeats in a length-dependent manner by concealing the 5' end of the flap that is necessary for both binding and cleavage by the protein encoded by this gene. Therefore, secondary structure can deter the protective function of this protein, leading to site-specific trinucleotide expansions.

Rad2/FEN1 (Phospho-Ser187) Antibody

12777-100ul 100ul
EUR 302.4

Rad2/FEN1 (Phospho-Ser187) Antibody

12777-50ul 50ul
EUR 224.4

Phospho-Rad2/FEN1 (Ser187) Antibody

AF8505 200ul
EUR 540

Human FEN1/ Flap endonuclease 1 ELISA Kit

E2908Hu 1 Kit
EUR 726

Mouse Flap endonuclease 1, Fen1 ELISA KIT

ELI-27512m 96 Tests
EUR 1038

Human Flap endonuclease 1, FEN1 ELISA KIT

ELI-09496h 96 Tests
EUR 988.8

Fen1 sgRNA CRISPR Lentivector set (Mouse)

K3646401 3 x 1.0 ug
EUR 406.8

FEN1 sgRNA CRISPR Lentivector set (Human)

K0774101 3 x 1.0 ug
EUR 406.8

Bovine Flap endonuclease 1, FEN1 ELISA KIT

ELI-12893b 96 Tests
EUR 1113.6

Chicken Flap endonuclease 1, FEN1 ELISA KIT

ELI-48473c 96 Tests
EUR 1113.6

Fen1 sgRNA CRISPR Lentivector (Rat) (Target 1)

K7115802 1.0 ug DNA
EUR 184.8

Fen1 sgRNA CRISPR Lentivector (Rat) (Target 2)

K7115803 1.0 ug DNA
EUR 184.8

Fen1 sgRNA CRISPR Lentivector (Rat) (Target 3)

K7115804 1.0 ug DNA
EUR 184.8

Fen1 ELISA Kit| Rat Flap endonuclease 1 ELISA Kit

EF018699 96 Tests
EUR 826.8

FEN-1 Polyclonal Conjugated Antibody

C40922 100ul
EUR 476.4

FEN1 recombinant monoclonal antibody

A5805 100ul X 3
EUR 714
Description: A recombinant monoclonal antibody from rabbit against human FEN1 for WB, IHC, IF,ELISA

Rabbit Anti Human Fen1 Polyclonal Antibody

CPBT-67395RH 0.1 mg
EUR 1132.8

Fen1 sgRNA CRISPR Lentivector (Mouse) (Target 1)

K3646402 1.0 ug DNA
EUR 184.8

Fen1 sgRNA CRISPR Lentivector (Mouse) (Target 2)

K3646403 1.0 ug DNA
EUR 184.8

Fen1 sgRNA CRISPR Lentivector (Mouse) (Target 3)

K3646404 1.0 ug DNA
EUR 184.8

FEN1 sgRNA CRISPR Lentivector (Human) (Target 1)

K0774102 1.0 ug DNA
EUR 184.8

FEN1 sgRNA CRISPR Lentivector (Human) (Target 2)

K0774103 1.0 ug DNA
EUR 184.8

FEN1 sgRNA CRISPR Lentivector (Human) (Target 3)

K0774104 1.0 ug DNA
EUR 184.8

Fen1 ELISA Kit| Mouse Flap endonuclease 1 ELISA Kit

EF014942 96 Tests
EUR 826.8

Recombinant Human Flap Endonuclease 1/FEN-1

C140-10ug 10ug
EUR 187.2
Description: Supplied as a 0.2 μm filtered solution of 50 mM Tris, 50mM NaCl, 1mM DDT, 10% Glycerol, pH 8.0.

Recombinant Human Flap Endonuclease 1/FEN-1

C140-1mg 1mg
EUR 2739.6
Description: Supplied as a 0.2 μm filtered solution of 50 mM Tris, 50mM NaCl, 1mM DDT, 10% Glycerol, pH 8.0.

Recombinant Human Flap Endonuclease 1/FEN-1

C140-500ug 500ug
EUR 1935.6
Description: Supplied as a 0.2 μm filtered solution of 50 mM Tris, 50mM NaCl, 1mM DDT, 10% Glycerol, pH 8.0.

Recombinant Human Flap Endonuclease 1/FEN-1

C140-50ug 50ug
EUR 442.8
Description: Supplied as a 0.2 μm filtered solution of 50 mM Tris, 50mM NaCl, 1mM DDT, 10% Glycerol, pH 8.0.

Phospho-Rad2/FEN1 (Ser187) Blocking Peptide

AF8505-BP 1mg
EUR 234

FEN1 ELISA Kit| Bovine Flap endonuclease 1 ELISA Kit

EF011390 96 Tests
EUR 826.8

Recombinant Pichia pastoris FEN1 Protein (aa 1-373)

VAng-Cr6600-1mgEcoli 1 mg (E. coli)
EUR 5487.6
Description: Pichia pastoris (strain GS115 / ATCC 20864) Flap endonuclease 1 (FEN1), recombinant protein.

Recombinant Pichia pastoris FEN1 Protein (aa 1-373)

VAng-Cr6600-500gEcoli 500 µg (E. coli)
EUR 3870
Description: Pichia pastoris (strain GS115 / ATCC 20864) Flap endonuclease 1 (FEN1), recombinant protein.

Recombinant Pichia pastoris FEN1 Protein (aa 1-373)

VAng-Cr6600-50gEcoli 50 µg (E. coli)
EUR 2650.8
Description: Pichia pastoris (strain GS115 / ATCC 20864) Flap endonuclease 1 (FEN1), recombinant protein.

Recombinant Giardia Lamblia FEN1 Protein (aa 1-361)

VAng-Cr4542-1mgEcoli 1 mg (E. coli)
EUR 5388
Description: Giardia Lamblia Flap endonuclease 1 (FEN1), recombinant protein.

Recombinant Giardia Lamblia FEN1 Protein (aa 1-361)

VAng-Cr4542-500gEcoli 500 µg (E. coli)
EUR 3820.8
Description: Giardia Lamblia Flap endonuclease 1 (FEN1), recombinant protein.

Recombinant Giardia Lamblia FEN1 Protein (aa 1-361)

VAng-Cr4542-50gEcoli 50 µg (E. coli)
EUR 2601.6
Description: Giardia Lamblia Flap endonuclease 1 (FEN1), recombinant protein.

FEN1 Lentiviral Vector (Rat) (CMV) (pLenti-GIII-CMV)

LV678703 1.0 ug DNA
EUR 818.4

FEN1 Lentiviral Vector (Rat) (UbC) (pLenti-GIII-UbC)

LV678707 1.0 ug DNA
EUR 818.4

FEN1 ELISA Kit| chicken Flap endonuclease 1 ELISA Kit

EF012306 96 Tests
EUR 826.8

Recombinant Leishmania Major FEN1 Protein (aa 1-395)

VAng-Wyb7487-1mgEcoli 1 mg (E. coli)
EUR 5652
Description: Leishmania Major Flap endonuclease 1, recombinant protein.

Recombinant Leishmania Major FEN1 Protein (aa 1-395)

VAng-Wyb7487-500gEcoli 500 µg (E. coli)
EUR 4002
Description: Leishmania Major Flap endonuclease 1, recombinant protein.

Recombinant Leishmania Major FEN1 Protein (aa 1-395)

VAng-Wyb7487-50gEcoli 50 µg (E. coli)
EUR 2733.6
Description: Leishmania Major Flap endonuclease 1, recombinant protein.

Recombinant Plasmodium Vivax FEN1 Protein

VAng-Wyb7861-inquire inquire Ask for price
Description: Plasmodium Vivax (strain Salvador I) Flap endonuclease 1, recombinant protein.

Fen1 sgRNA CRISPR/Cas9 All-in-One Lentivector set (Rat)

K7115805 3 x 1.0 ug
EUR 451.2

Recombinant Trypanosoma Cruzi FEN1 Protein (aa 1-393)

VAng-Wyb8422-100gBaculovirus 100 µg (Baculovirus)
EUR 5421.6
Description: Trypanosoma Cruzi (strain CL Brener) Flap endonuclease 1 protein, recombinant protein.

Recombinant Trypanosoma Cruzi FEN1 Protein (aa 1-393)

VAng-Wyb8422-500gBaculovirus 500 µg (Baculovirus)
EUR 7022.4
Description: Trypanosoma Cruzi (strain CL Brener) Flap endonuclease 1 protein, recombinant protein.

Recombinant Trypanosoma Cruzi FEN1 Protein (aa 1-393)

VAng-Wyb8422-50gBaculovirus 50 µg (Baculovirus)
EUR 3771.6
Description: Trypanosoma Cruzi (strain CL Brener) Flap endonuclease 1 protein, recombinant protein.

FEN1 Lentiviral Vector (Rat) (EF1a) (pLenti-GIII-EF1a)

LV678708 1.0 ug DNA
EUR 818.4

Recombinant Trypanosoma Brucei FEN1 Protein (aa 1-393)

VAng-Wyb8072-100gBaculovirus 100 µg (Baculovirus)
EUR 5421.6
Description: Trypanosoma Brucei brucei (strain 927/4 GUTat10.1) Flap endonuclease 1 protein, recombinant protein.

Recombinant Trypanosoma Brucei FEN1 Protein (aa 1-393)

VAng-Wyb8072-500gBaculovirus 500 µg (Baculovirus)
EUR 7022.4
Description: Trypanosoma Brucei brucei (strain 927/4 GUTat10.1) Flap endonuclease 1 protein, recombinant protein.

Recombinant Trypanosoma Brucei FEN1 Protein (aa 1-393)

VAng-Wyb8072-50gBaculovirus 50 µg (Baculovirus)
EUR 3771.6
Description: Trypanosoma Brucei brucei (strain 927/4 GUTat10.1) Flap endonuclease 1 protein, recombinant protein.

Recombinant Aspergillus Niger FEN1 Protein (aa 1-395) [His]

VAng-Wyb3890-1mg 1 mg
EUR 5732.4
Description: Aspergillus Niger (strain CBS 513.88 / FGSC A1513) Flap Structure-Specific Endonuclease 1, recombinant protein.

Fen1 sgRNA CRISPR/Cas9 All-in-One Lentivector set (Mouse)

K3646405 3 x 1.0 ug
EUR 451.2

FEN1 sgRNA CRISPR/Cas9 All-in-One Lentivector set (Human)

K0774105 3 x 1.0 ug
EUR 451.2

Recombinant Human FEN1 Protein, Untagged, E.coli-1mg

QP11866-1mg 1mg
EUR 4386

Recombinant Human FEN1 Protein, Untagged, E.coli-5ug

QP11866-5ug 5ug
EUR 186

Recombinant Leishmania Infantum FEN1 Protein (aa 1-395)

VAng-Wyb7444-1mgEcoli 1 mg (E. coli)
EUR 5652
Description: Leishmania Infantum Flap endonuclease 1, recombinant protein.

Recombinant Leishmania Infantum FEN1 Protein (aa 1-395)

VAng-Wyb7444-500gEcoli 500 µg (E. coli)
EUR 4002
Description: Leishmania Infantum Flap endonuclease 1, recombinant protein.

Recombinant Leishmania Infantum FEN1 Protein (aa 1-395)

VAng-Wyb7444-50gEcoli 50 µg (E. coli)
EUR 2733.6
Description: Leishmania Infantum Flap endonuclease 1, recombinant protein.

Recombinant Plasmodium Knowlesi FEN1 Protein (aa 1-595)

VAng-Wyb7808-inquire inquire Ask for price
Description: Plasmodium Knowlesi (strain H) Flap endonuclease 1, partial, recombinant protein.

Recombinant Plasmodium Yoelii FEN1 Protein (aa 1-480) (E. coli)

VAng-Wyb7918-inquire inquire Ask for price
Description: Plasmodium Yoelii Flap endonuclease 1 protein, recombinant protein.

Recombinant Trypanosoma Brucei FEN1 Protein (aa 1-393) [His]

VAng-Wyb8074-1mg 1 mg
EUR 5788.8
Description: Trypanosoma Brucei brucei (strain 927/4 GUTat10.1) Flap Structure-Specific Endonuclease 1 protein, recombinant protein.

Recombinant Aspergillus Flavus FEN1 Protein (aa 1-395) [His]

VAng-Wyb3815-1mg 1 mg
EUR 5732.4
Description: Aspergillus Flavus (strain ATCC 200026 / FGSC A1120 / NRRL 3357 / JCM 12722 / SRRC 167) Flap Structure-Specific Endonuclease 1, recombinant protein.

Fenson

S-4247 1ML
EUR 168

Fen1 sgRNA CRISPR/Cas9 All-in-One Lentivector (Rat) (Target 1)

K7115806 1.0 ug DNA
EUR 200.4

Fen1 sgRNA CRISPR/Cas9 All-in-One Lentivector (Rat) (Target 2)

K7115807 1.0 ug DNA
EUR 200.4

Fen1 sgRNA CRISPR/Cas9 All-in-One Lentivector (Rat) (Target 3)

K7115808 1.0 ug DNA
EUR 200.4

FEN1P1 Lentiviral Vector (Human) (CMV) (pLenti-GIII-CMV)

LV761441 1.0 ug DNA Ask for price

FEN1P1 Lentiviral Vector (Human) (UbC) (pLenti-GIII-UbC)

LV761445 1.0 ug DNA Ask for price

Recombinant Human FEN1 Protein, Untagged, E.coli-20ug

QP11866-20ug 20ug
EUR 241.2

Recombinant Plasmodium Berghei FEN1 Protein (aa 1-478) (E. coli)

VAng-Wyb7623-inquire inquire Ask for price
Description: Plasmodium Berghei (strain Anka) Flap endonuclease 1 protein, recombinant protein.

Recombinant Plasmodium Yoelii FEN1 Protein (aa 1-480) (Yeast)

VAng-Wyb7917-inquire inquire Ask for price
Description: Plasmodium Yoelii Flap endonuclease 1 protein, recombinant protein.

Recombinant Aspergillus Terreus FEN1 Protein (aa 1-395) [His]

VAng-Wyb4050-1mg 1 mg
EUR 5732.4
Description: Aspergillus Terreus (strain NIH 2624 / FGSC A1156) Flap Structure-Specific Endonuclease 1, recombinant protein.

Rat Flap Structure Specific Endonuclease 1 (FEN1) ELISA Kit

20-abx391344
  • EUR 8853.60
  • EUR 4719.60
  • EUR 1093.20
  • 10 × 96 tests
  • 5 × 96 tests
  • 96 tests

Recombinant Plasmodium Berghei FEN1 Protein (aa 1-478) (Yeast)

VAng-Wyb7622-inquire inquire Ask for price
Description: Plasmodium Berghei (strain Anka) Flap endonuclease 1 protein, recombinant protein.

Recombinant Plasmodium Chabaudi FEN1 Protein (aa 1-479) (E. coli)

VAng-Wyb7723-inquire inquire Ask for price
Description: Plasmodium Chabaudi Flap endonuclease 1 protein, recombinant protein.

Recombinant Plasmodium Falciparum FEN1 Protein (aa 1-650)

VAng-Wyb6429-inquire inquire Ask for price
Description: Plasmodium Falciparum Flap endonuclease 1, recombinant protein.

Recombinant Plasmodium Falciparum FEN1 Protein (aa 1-672)

VAng-Wyb6430-inquire inquire Ask for price
Description: Plasmodium Falciparum (isolate 3D7) Flap endonuclease 1, partial, recombinant protein.

Recombinant Aspergillus Clavatus FEN1 Protein (aa 1-395) [His]

VAng-Wyb3772-1mg 1 mg
EUR 5732.4
Description: Aspergillus Clavatus (strain ATCC 1007 / CBS 513.65 / DSM 816 / NCTC 3887 / NRRL 1) Flap Structure-Specific Endonuclease 1, recombinant protein.

Fenobam

B7035-10 10 mg
EUR 303.6

Fenobam

B7035-50 50 mg
EUR 1082.4

Fenobam

HY-101478 1mg
EUR 157.2

Fenuron

S-2011 1ML
EUR 100.8

Flap Structure Specific Endonuclease 1 (FEN1) Antibody

abx233075-100ug 100 ug
EUR 577.2

Flap Structure Specific Endonuclease 1 (FEN1) Antibody

abx146057-100ug 100 ug
EUR 469.2

Flap Structure Specific Endonuclease 1 (FEN1) Antibody

20-abx013480
  • EUR 376.80
  • EUR 117.60
  • EUR 477.60
  • EUR 594.00
  • 100 ug
  • 10 ug
  • 200 ug
  • 300 µg

Flap Structure Specific Endonuclease 1 (FEN1) Antibody

20-abx001089
  • EUR 493.20
  • EUR 710.40
  • EUR 218.40
  • EUR 376.80
  • 100 ul
  • 200 ul
  • 20 ul
  • 50 ul

Flap Structure Specific Endonuclease 1 (FEN1) Antibody

abx412568-01mg 0.1 mg
EUR 844.8

Flap Structure Specific Endonuclease 1 (FEN1) Antibody

20-abx112557
  • EUR 878.40
  • EUR 477.60
  • 150 ul
  • 50 ul

Flap Structure Specific Endonuclease 1 (FEN1) Antibody

20-abx126909
  • EUR 493.20
  • EUR 710.40
  • 100 ul
  • 200 ul

Flap Structure Specific Endonuclease 1 (FEN1) Antibody

abx117052-100ug 100 ug
EUR 560.4

Flap Structure Specific Endonuclease 1 (FEN1) Antibody

20-abx009270
  • EUR 360.00
  • EUR 526.80
  • EUR 226.80
  • 100 ul
  • 200 ul
  • 30 ul

Flap Structure Specific Endonuclease 1 (FEN1) Antibody

abx031748-400ul 400 ul
EUR 627.6

Prokaryotic expression of PE8 protein from Mycobacterium tuberculosis (H37Rv) and preparation of its polyclonal antibody in rabbits

 

Goal To clone proline-glutamate 8 (PE8) gene phase from Mycobacterium tuberculosis (H37Rv), assemble the recombinant plasmid pET28a-PE8, categorical recombinant PE8 protein, and put together its polyclonal antibody. Strategies Utilizing a regular homologous recombination cloning expertise, we cloned the PE8 gene into the prokaryotic vector pET28a. After sequence affirmation, it was reworked into E. coli BL21 (DE3) and handled with 0.5 mmol/L isopropyl-beta-D-thiogalactopyranoside (IPTG) to induce protein expression.

We purified and renatured the recombinant PE8 protein, and immunized New Zealand rabbits to arrange the polyclonal antibody. Antibody titer was decided by oblique ELISA and the specificity was evaluated by Western blot evaluation. Outcomes The recombinant plasmid pET28a-PE8 was efficiently constructed, and the PE8 protein was primarily expressed in an inclusion physique in E. coli. After renaturation and purification, a purity of about 90% of the recombinant protein was achieved.

The titer of the polyclonal antibody was greater than 1:430 080. The polyclonal antibody may particularly acknowledge the recombinant PE8 protein. Conclusion We have now efficiently expressed and purified recombinant PE8 protein, which might be additional utilized to generate PE8 polyclonal antibody with acceptable titer and specificity.

A preliminary analysis of a regionally produced biotinylated polyclonal anti-rabies antibody for direct speedy immunohistochemical check (DRIT) within the Philippines

 

  • Rabies is a deadly zoonotic illness endemic in creating nations of Asia and Africa. Lately, the direct speedy immunohistochemical check (DRIT) was advisable by the World Well being Group (WHO) and the World Group for Animal Well being (OIE) as a diagnostic check for rabies. Subsequently, a biotinylated polyclonal antibody (pAb) in opposition to the rabies lyssavirus (RABV) nucleoprotein was developed utilizing a plasmid cDNA vaccine derived from a problem virus normal 11 pressure.

 

  • A preliminary analysis on the efficacy of this reagent in recognizing the Philippine RABV pressure was examined utilizing banked canine hippocampal tissue samples with DRIT and the outcomes have been in comparison with dFAT. The consequences of acetone and formalin fixation on DRIT have been additionally assessed by way of immunoreactivity scores of the specimens.

 

  • Of the 142 samples examined, 104 examined constructive and 38 detrimental utilizing each dFAT and DRIT, displaying 100% settlement between the 2 diagnostic procedures. Furthermore, no false constructive or false detrimental outcomes have been noticed utilizing acetone and formalin fixation. Thus, regionally ready biotinylated pAb from plasmid cDNA can be utilized for DRIT, particularly in resource-limited laboratories within the Philippines.

 

  • Nonetheless, these outcomes ought to be confirmed with a extra thorough analysis of this method, and the vary of detection must be additional evaluated in a bigger panel of animal samples and on different lyssaviruses.

 

Acetylcholinesterase (AChE) polyclonal antibody from hybrid catfish (C. macrocephalus × C. gariepinus): Specification, sensitivity and cross reactivity

 

AChE (acetylcholinesterase) is usually categorized as a selected biomarker of pesticide publicity. The goal of this examine was to supply AChE polyclonal antibody from hybrid catfish that have been uncovered to business glyphosate. The hybrid catfish was uncovered to glyphosate (0.75 mL/L) for 24 h. After that, the fish mind was dissected, AChE was extracted and purified by hydroxyapatite column chromatography and eluted with 0.2 M potassium phosphate buffer pH 6.8. This protocol gave 70% yield.

Then, the mind extract was characterised utilizing 10% SDS-PAGE and Western blot probed with business polyclonal antibody particular to AChE (PAb-AChE). The protein, 71 kDa, was then used as an antigen to immunize mice for antibody manufacturing.

The polyclonal antibody (PAb) was characterised utilizing dot blot, Western blot and immunohistochemistry for immunolocalization of AChE in hybrid catfish uncovered to glyphosate. We discovered that the suitable dilution of antibody for each dot blot and Western blot was 1:3500, and 1:2500 for immunohistochemistry.

Cross reactivity testing confirmed that PAb-AChE can be utilized with AChE from striped snakehead fish on the identical dilution as used with AChE from hybrid catfish. It was concluded that PAb particular to hybrid catfish AChE from this work was extremely particular and delicate, and might cross-react with striped snakehead fish AChE. Thus, this polyclonal antibody could also be utilized in monitoring glyphosate publicity in hybrid catfish and striped snakehead fish.

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