Efficient detection of eukaryotic calcium-sensing receptor

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In direction of middle-up evaluation of polyclonal antibodies: subclass-specific N-glycosylation profiling of murine immunoglobulin G (IgG) by the use of HPLC-MS

 

  • Lately, superior HPLC-MS methods based mostly on intact protein (“top-down”) or protein subunit (“middle-up/middle-down”) evaluation have been applied for the characterization of therapeutic monoclonal antibodies. Right here, we assess feasibility of middle-up/middle-down evaluation for polyclonal IgGs exhibiting in depth sequence variability. Particularly, we addressed IgGs from mouse, representing an essential mannequin system in immunological investigations.
  • To acquire Fc/2 parts as conserved subunits of IgGs, we made use of the bacterial protease SpeB. For this function, we initially decided SpeB cleavage websites in murine IgGs. The ensuing Fc/2 parts attribute of various subclasses had been subsequently analysed by ion-pair reversed-phase HPLC hyphenated to high-resolution mass spectrometry. This enabled simultaneous relative quantification of IgG subclasses and their N-glycosylation variants, each of which affect IgG effector features.
  • To evaluate methodology capabilities in an immunological context, we utilized the analytical workflow to polyclonal antibodies obtained from BALB/c mice immunized with the grass pollen allergen Phl p 6. The research revealed a shift in IgG subclasses and Fc-glycosylation patterns in whole and antigen-specific IgGs from totally different mouse cohorts, respectively. Finally, Fc/2 characterization might reveal different protein modifications together with oxidation, amino acid exchanges, and C-terminal lysine, and will thus be applied for high quality management of practical antibodies.

Environment friendly detection of eukaryotic calcium-sensing receptor (CaSR) by polyclonal antibody towards prokaryotic expressed truncated CaSR

 

Calcium-sensing receptor (CaSR), which is best identified for its motion as regulating calcium homeostasis, can bind varied ligands. To facilitate analysis on CaSR and perceive the receptor’s operate additional, an in silico designed truncated protein was developed. The ensuing protein folding indicated that 99% of predicted three dimensional (3D) construction residues are positioned in favored and allowed Ramachandran plots. Nonetheless, it was discovered that such protein doesn’t fold correctly when expressed in prokaryotic host cells.

Thioredoxin (Trx) tag was conjugated to extend the ultimate protein’s solubility, which may assist acquire the soluble antigen with higher immunogenic properties. The truncated recombinant proteins had been expressed and purified in two kinds (Trx-CaSR: RR19 and CaSR: RRJ19). The polyclonal antibody was induced by the rabbit immunization with the type of RR19. Western blot on mouse kidney lysates evidenced the correct immune recognition of the receptor by the produced antibody.

The specificity and sensitivity of antibodies had been additionally assayed by immunohistofluorescence. These experiments affirmed antibody’s means to point the receptor on the cell floor in native type and the potential for making use of such antibodies in additional mobile and tissue assays.

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Doxorubicin Hydrochloride

40410004-1 10 mg
EUR 136.34

Doxorubicin Hydrochloride

40410004-2 25 mg
EUR 273.17

Doxorubicin Hydrochloride

D558000 100mg
EUR 136
Description: 25316-40-9

Doxorubicin hydrochloride

GA4969-100MG 100 mg
EUR 217.2

Doxorubicin hydrochloride

GA4969-10MG 10 mg
EUR 93.6

Doxorubicin hydrochloride

GA4969-25MG 25 mg
EUR 117.6

Doxorubicin hydrochloride

GA4969-50MG 50 mg
EUR 151.2

Doxorubicin hydrochloride

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Doxorubicin Hydrochloride

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Doxorubicin hydrochloride

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Doxorubicin (hydrochloride)

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EUR 176.4

Doxorubicin Hydrochloride

SIH-390-10MG 10 mg
EUR 129
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Doxorubicin hydrochloride, 98%

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EUR 117.34
Description: C27H29NO11•HCl

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EUR 234.67
Description: C27H29NO11•HCl

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Doxorubicin hydrochloride, USP grade

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Doxorubicin

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EUR 48
Description: Topo II inhibitor,immunosuppresive antineoplastic antibiotic

Doxorubicin

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Doxorubicin

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Doxorubicin

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Description: Topo II inhibitor,immunosuppresive antineoplastic antibiotic

Doxorubicin

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Description: Topo II inhibitor,immunosuppresive antineoplastic antibiotic

Doxorubicin

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Doxorubicin

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Doxorubicin HCl

20-abx184016
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Doxorubicin HCl

abx076623-25ml 25 ml
EUR 187.5

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EUR 118.75

Doxorubicin 10mM

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406622 1.0mg
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Doxorubicin HCl, 100mg

R2531 100mg
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Doxorubicin HCl, 100mg

R2531-100mg each
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R2531-10mg each
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Adriamycin (Doxorubicin)

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Doxorubicin Free Base

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Doxorubicin (Adriamycin) HCl

A1832-10 10 mg
EUR 36
Description: Antitumour antibiotic,inhibits TOPO II.

Doxorubicin (Adriamycin) HCl

A1832-100 100 mg
EUR 128
Description: Antitumour antibiotic,inhibits TOPO II.

Doxorubicin (Adriamycin) HCl

A1832-25 25 mg
EUR 52
Description: Antitumour antibiotic,inhibits TOPO II.

Doxorubicin (Adriamycin) HCl

A1832-5.1 10 mM (in 1mL DMSO)
EUR 44
Description: Antitumour antibiotic,inhibits TOPO II.

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Description: 677017-23-1

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7-Deoxy Doxorubicin Aglycone (> 75%)

D232910 10mg
EUR 299
Description: 38554-25-5

PEGylated Liposomal Doxorubicin- 2 mg

PHPC002DX 2 mg
EUR 140.62
Description: This product includes 1 ml of liposomal doxorubicin at concentrations of 2 mg/ml doxorubicin and 16 mg/ml of lipids.

PEGylated Liposomal Doxorubicin- 20 mg

PHPC020DX 20 mg
EUR 358.62
Description: This product includes 10 ml of liposomal doxorubicin at concentrations of 2 mg/ml doxorubicin and 16 mg/ml of lipids.

Doxorubicin-hydrazone-caproyl-maleimide

D558040 100mg
EUR 1800
Description: 151038-96-9

Human Doxorubicin-Stimulated HeLa Whole Cell Lysate

IHUHELADTLW500UG each
EUR 327
Description: Human Doxorubicin-Stimulated HeLa Whole Cell Lysate

Human Doxorubicin-Stimulated HeLa Nuclear Extract Lysate

IHUHELADTLN200UG each
EUR 427
Description: Human Doxorubicin-Stimulated HeLa Nuclear Extract Lysate

7,8-Desacetyl-9,10-dehydro Daunorubicinone (~90%)(Doxorubicin Impurity)

D288680 10mg
EUR 4281
Description: 1159977-24-8

Human HeLa (Cervix Adenocarcinoma) Whole Cell Lysate - Doxorubicin Stimulated

HCL-2010 100ug
EUR 255.6

6,8,11-Trihydroxy-1-methoxy-5,12-naphthacenedione (Doxorubicin Impurity)

T795210 10mg
EUR 244
Description: 64845-67-6

Human HeLa (Cervix Adenocarcinoma) Cell Nuclear Extract - Doxorubicin Stimulated

HCL-2004 100ug
EUR 270

Des-[(4-Amino-2,3,6-trideoxy-hexapyranosyl)oxy)-1-methyl] 2-Carboxylate Doxorubicin

D558001 100mg
EUR 4500

Doxorubicin.HCl

1527-5 each
EUR 183.6

Doxorubicinol (>90%)

D558025 2.5mg
EUR 1929

Doxorubicinol

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Doxorubicinone

D559070 25mg
EUR 244
Description: 24385-10-2

Doxorubicinone

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Doxorubicinone

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Description: Doxorubicinone

Doxorubicinone

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Description: Doxorubicinone

APY29 Hydrochloride Salt

A735422 100mg
EUR 224

3-N-Carboxylic Acid 1-beta-D-Glucuronide-[4-(methyl)phenyl]carbamate Ester Doxorubicin

C177855 50mg
EUR 11200
Description: 211364-63-5

Axomadol Hydrochloride Salt

A583490 100mg
EUR 11200
Description: 187219-95-0

Morphine Hydrochloride Salt

M652280 100mg
EUR 500
Description: 52-26-6

OPC 4392 Hydrochloride Salt

O667500 100mg
EUR 193

Erlotinib, Hydrochloride Salt

1588-100 each
EUR 202.8

Erlotinib, Hydrochloride Salt

1588-1000 each
EUR 477.6

Carazolol Hydrochloride Salt

C175810 100mg
EUR 138
Description: 51997-43-4

Carazolol hydrochloride salt

574509 50.0mg
EUR 285

Tofenacin Hydrochloride Salt

T528500 10mg
EUR 339

Osutidine Hydrochloride Salt

O703910 100mg
EUR 1696

CHIR 99021 Hydrochloride Salt

C292157 100mg
EUR 178

Difloxacin Hydrochloride Salt

D445600 100mg
EUR 460
Description: 91296-86-5

Dimethacrine Hydrochloride Salt

D460270 100mg
EUR 4500
Description: 100000-17-7

Ramifenazone Hydrochloride Salt

R110500 100mg
EUR 86
Description: 18342-39-7

Pexidartinib Hydrochloride Salt

P293640 100mg
EUR 800
Description: 2040295-03-0

Volinanserin Hydrochloride Salt

V755000 1mg
EUR 598

Lysinoalanine Hydrochloride Salt

L488650 10mg
EUR 219
Description: 93113-28-1

7,8,9,10-Dehydro Doxorubicinone (~70%)

D229580 10mg
EUR 5528
Description: 1159977-23-7

Aposcopolamine Hydrochloride Salt

A729005 100mg
EUR 230
Description: 890416-03-2

I-CBP 112 Hydrochloride Salt

I163880 5mg
EUR 403
Description: 2147701-33-3

Bleomycin A5 Hydrochloride Salt

B595750 10mg
EUR 230
Description: 55658-47-4

Erlotinib, Hydrochloride Salt, >99%

BC026-101 1g
EUR 327.6

Erlotinib, Hydrochloride Salt, >99%

BC026-102 2g
EUR 465.6

Erlotinib, Hydrochloride Salt, >99%

BC026-105 5g
EUR 684

Dopamine -13C8 Hydrochloride Salt

D533784 25mg
EUR 7600

Peramine Hydrochloride Salt-d3

P285302 10mg
EUR 184

Bromo Didehydro Doxorubicinone

B682880 25mg
EUR 1200
Description: 63626-06-2

Carazolol-d6, Hydrochloride Salt

C175812 50mg
EUR 181
Description: 1184973-07-6

Tofenacin Hydrochloride Salt-d4

T528502 10mg
EUR 190

17-DMAG, Hydrochloride Salt, >98%

BC024-001 1mg
EUR 213.6

17-DMAG, Hydrochloride Salt, >98%

BC024-005 5mg
EUR 343.2

17-DMAG, Hydrochloride Salt, >98%

BC024-010 10mg
EUR 489.6

17-DMAG, Hydrochloride Salt, >98%

BC024-025 25mg Ask for price

17-DMAG, Hydrochloride Salt, >99%

BC024-100 100mg Ask for price

17-DMAG, Hydrochloride Salt, >99%

BC024-101 1g Ask for price

17-DMAG, Hydrochloride Salt, >99%

BC024-250 250mg Ask for price

Flupirtine-d4 Hydrochloride Salt

F598512 10mg
EUR 215
Description: 1324717-75-0

Difloxacin-d3 Hydrochloride Salt

D445601 10mg
EUR 127
Description: 1173021-89-0

rac-Emixustat Hydrochloride Salt

B224873 1g
EUR 3000
Description: 1222169-23-4

Abeprazan dimer Hydrochloride salt

A642330 100mg
EUR 17000

cis-Mefentanyl Hydrochloride Salt

M205505 50mg
EUR 232
Description: 78995-18-3

rac Metanephrine Hydrochloride Salt

M258760 100mg
EUR 207
Description: 881-95-8

b-Isothipendyl Hydrochloride Salt

I902751 100mg
EUR 845
Description: 132962-42-6

Ondansetron-d3 Hydrochloride Salt

O655002 10mg
EUR 193
Description: 1346605-02-4

OSU-03012, Hydrochloride Salt, >98%

BC061-005 5mg
EUR 375.6

OSU-03012, Hydrochloride Salt, >98%

BC061-025 25mg
EUR 634.8

MEL3 Isomer I Hydrochloride Salt

M155671 50mg
EUR 15000

MEL3 Isomer II Hydrochloride Salt

M155672 50mg
EUR 7600

Dimethacrine Hydrochloride Salt-d3

D460272 100mg
EUR 9200

Linear Daptomycin Hydrochloride Salt

L466475 2.5mg
EUR 269

Iso Desloratadine Hydrochloride Salt

I815005 10mg
EUR 1185

Volinanserin-d4 Hydrochloride Salt

V755002 10mg
EUR 265
Description: 1217617-73-6

2'-Nor Thiamine Hydrochloride Salt

N830500 100mg
EUR 249
Description: 49614-72-4

Tamsulosin Diether Hydrochloride Salt

E736620 100mg
EUR 695

Apovincaminic Acid Hydrochloride Salt

A729250 500mg
EUR 193
Description: 72296-47-0

Keto-Isoxsuprine Hydrochloride Salt

K606400 100mg
EUR 1200

Synephrine-13C2,15N Hydrochloride Salt

S920002 0.1mg
EUR 1722
Description: 1329499-30-0

Apovincaminic acid hydrochloride salt

T37406-10mg 10mg Ask for price
Description: Apovincaminic acid hydrochloride salt

Apovincaminic acid hydrochloride salt

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Description: Apovincaminic acid hydrochloride salt

Apovincaminic acid hydrochloride salt

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Description: Apovincaminic acid hydrochloride salt

Apovincaminic acid hydrochloride salt

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Description: Apovincaminic acid hydrochloride salt

Apovincaminic acid hydrochloride salt

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Description: Apovincaminic acid hydrochloride salt

Reticuline Hydrochloride Salt (>90% ee)

R188500 0.5mg
EUR 2070
Description: 903-91-3

(R)-Norphenylephrine Hydrochloride Salt

N825510 25mg
EUR 3000
Description: 636-87-3

Dehydroxy Mirabegron Hydrochloride Salt

D230170 250mg
EUR 201
Description: 1581284-79-8

5-Nitroisoindoline Hydrochloride Salt

N496370 1g
EUR 75
Description: 400727-69-7

Azetidin-3-ol Hydrochloride Salt

A813200 25g
EUR 357
Description: 18621-18-6

Validamycin A (>70%) Hydrochloride Salt

V943430 100mg
EUR 322

CJ-023423 Hydrochloride Salt, Hydrate

C535808 1mg
EUR 2110

Desmethyl Sibutramine, Hydrochloride Salt

D292170 10mg
EUR 221
Description: 84467-94-7

Inhibitory Potential of Polyclonal Camel Antibodies towards New Delhi Metallo-β-lactamase-1 (NDM-1)

New Delhi Metallo-β-lactamase-1 (NDM-1) is probably the most prevalent kind of metallo-β-lactamase, in a position to hydrolyze virtually all antibiotics of the β-lactam group, resulting in multidrug-resistant micro organism. Up to now, there aren’t any clinically related inhibitors to combat NDM-1. Using dromedary polyclonal antibody inhibitors towards NDM-1 represents a promising new class of molecules with inhibitory exercise.

Within the present research, immunoreactivities of dromedary Immunoglobulin G (IgG) isotypes containing heavy-chain and standard antibodies had been examined after profitable immunization of dromedary utilizing growing quantities of the recombinant NDM-1 enzyme. Inhibition kinetic assays, carried out utilizing a spectrophotometric methodology with nitrocefin as a reporter substrate, demonstrated that IgG1, IgG2, and IgG3 had been in a position to inhibit not solely the hydrolytic exercise of NDM-1 but in addition Verona integron-encoded metallo-β-lactamase (VIM-1) (subclass B1) and L1 metallo-β-lactamase (L1) (subclass B3) with inhibitory focus (IC50) values starting from 100 to 0.04 μM.

Investigations on the flexibility of IgG subclasses to cut back the expansion of recombinant Escherichia coli BL21(DE3)/codon plus cells containing the recombinant plasmid expressing NDM-1, L1, or VIM-1 confirmed that the addition of IgGs (four and eight mg/L) to the cell tradition was unable to revive the susceptibility of carbapenems.

Apparently, IgGs had been in a position to work together with NDM-1, L1, and VIM-1 when examined on the periplasm extract of every cultured pressure. The inhibitory focus was within the micromolar vary for all β-lactams examined. A visualization of the 3D structural foundation utilizing the three enzyme Protein Knowledge Financial institution (PDB) recordsdata helps preliminarily the recorded inhibition of the three MBLs.

A Easy and Environment friendly Genetic Immunization Protocol for the Manufacturing of Extremely Particular Polyclonal and Monoclonal Antibodies towards the Native Type of Mammalian Proteins

 

We’ve got generated polyclonal and monoclonal antibodies by genetic immunization over the past twenty years. On this paper, we current our most profitable methodology acquired over these years and current the animals by which we obtained the very best charges of success. The method introduced is handy, simple, inexpensive, and generates antibodies towards mammalian proteins of their native type.

This protocol requires neither costly gear, resembling a gene gun, nor subtle methods such because the conjugation of gold microspheres, electroporation, or surgical procedure to inject in lymph nodes. The protocol introduced makes use of merely the purified plasmid expressing the protein of curiosity below a powerful promoter, which is injected at intramuscular and intradermal websites.

This method was examined in 5 species. Guinea pigs had been the animals of alternative for the manufacturing of polyclonal antibodies. Monoclonal antibodies could possibly be generated in mice by giving, as a final injection, a suspension of transfected cells. The antibodies detected their antigens of their native kinds. They had been extremely particular with very low non-specific background ranges, as assessed by immune-blots, immunocytochemistry, immunohistochemistry and circulation cytometry. We current herein an in depth and easy process to efficiently elevate particular antibodies towards native proteins.

Manufacturing of a polyclonal antibody towards inosine-uridine preferring nucleoside hydrolase of Acanthamoeba castellanii and its entry to prognosis of Acanthamoeba keratitis

 

Acanthamoeba keratitis (AK) is a uncommon illness however its prevalence all through the globe continues to develop, primarily as a result of elevated contact lens utilization. Since early-stage signs related to AK carefully resemble these from different corneal infections, correct prognosis is tough and this typically leads to delayed therapy and exacerbation of the illness, which might result in everlasting visible impairment. Accordingly, creating a speedy Acanthamoeba-specific diagnostic methodology is very desired.

Within the current research, a speedy and . Among the many huge portions of proteins secreted by the pathogenic Acanthamoeba, an open studying body of the inosine-uridine preferring nucleoside hyddifferential methodology for AK prognosis was developed utilizing the secretory proteins derived from the pathogenic Acanthamoebarolase (IPNH) gene was obtained. After expressing and purifying the IPNH protein utilizing the pGEX 4T-Three vector system, mice had been immunized with the purified proteins for polyclonal antibody technology.

Western blot was carried out utilizing protein lysates of the human corneal cell, non-pathogenic amoeba, pathogenic amoeba, and scientific amoeba isolate together with lysates from different causes of keratitis resembling Staphylococcus aureus, Pseudomonas aeruginosa, and Fusarium solani to verify Acanthamoeba-specificity.

Western blot utilizing the polyclonal IPNH antibody revealed that IPNH was Acanthamoeba-specific since these proteins had been solely noticed in lysates of Acanthamoeba origin or its tradition media. Our findings point out that the IPNH antibody of Acanthamoeba might function a possible agent for speedy and differential AK prognosis.

RIPA Lysis Buffer (Strong)

HY-K1001 100 mL
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Carbol Fuchsin (ZN,Strong)

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Description: Carbol Fuchsin (ZN,Strong)

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Description: Carbol Fuchsin (ZN,Strong)

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HiDecal (Strong decalcifying solution)

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EUR 21.95
Description: HiDecal (Strong decalcifying solution)

Arg-SEC Mobile Phase(Strong)

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EUR 105

Pierce Seal Strong Roll - 1ROLL

PCR0636 1ROLL
EUR 1314.9

Pierce Seal Strong Sheets - PK100

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EUR 140.4

replacement tip Kit strong thick

INS2090 EACH
EUR 8.33

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M1237-500G 1 unit
EUR 80.55
Description: Modified Duncan Strong (DS) Medium

Pierce Seal Strong Roll610mX78mm - 1ROLL

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Western Stripping buffer (Strong alkaline)

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EUR 9.6

Western Stripping buffer (Strong alkaline)

EZWB03-1-500mL 500mL
EUR 28.8

Tweezers Fine Strong CF Tips - EACH

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EUR 37.6

Tweezers Flat Strong CF Tips - EACH

INS5100 EACH
EUR 43.92

PCR Foil Seal Strong Sheets - PK100

PCR0528 PK100
EUR 211.95

EP Reagent Sodium Hypochlorite Sol. Strong - 500ML

10816005 500ML
EUR 136.35

Pierce Seal Strong Sample Roll - 1ROLL

PCR0630 1ROLL
EUR 59.63

replacement tip Kit strong thick - EACH

INS2058 EACH
EUR 11.65

replacement tip Kit strong point - EACH

INS2094 EACH
EUR 11.65

Modified Duncan Strong (DS) HiVeg Medium

MV1237-500G 1 unit
EUR 80.55
Description: Modified Duncan Strong (DS) HiVeg Medium

Strong RIPA Lysis Buffer (without inhibitors)

EZPS03-2 100mL
EUR 12.6

EP Reagent Sodium Hydroxide Sol. Strong - 1L

1081404 1L
EUR 191.7

Tweezers, Rubis Sturdy, Strong Pointed, 115mm, 4.5€

25046-1 1EA
EUR 48

Silver Protein for Histology, Strong (not certified)

25108-25 25g
EUR 431
Description: 9015-51-4

Silver Protein for Histology, Strong (not certified)

25108-5 5g
EUR 113
Description: 9015-51-4

Magnetic Base Support Z w/ Strong Magnet - 34.5mm

M-R-1013161 1 UNIT
EUR 46
Description: Magnetic Base Support Z w/ Strong Magnet - 34.5mm

Magnetic Base Support Z w/ Strong Magnet - 27.5mm

M-R-1015229 1 UNIT
EUR 46
Description: Magnetic Base Support Z w/ Strong Magnet - 27.5mm

UCW5072, Low TOC Strong Base Anion Resin, OH Form

50240-1 1000ml
EUR 192

UCW5072, Low TOC Strong Base Anion Resin, OH Form

50240-250 250ml
EUR 62

A510, Type 2 Macroporous Strong Base Rsin, Chloride Form

50223-1 1000ml
EUR 144
Description: 69011-15-0

DiagAg™ Strong Anion Exchange Agarose Particles, 40 μm

DAG-CL23-08 25 mL
EUR 1150

A510, Type 2 Macroporous Strong Base Resin, Chloride Form

50223-250 250ml
EUR 47
Description: 69011-15-0

Genistin

1535-10 each
EUR 196.8

Genistin

G350020 10mg
EUR 63
Description: 529-59-9

Genistin

GC2802-10 10
EUR 242.4

Genistin

GC2802-100 100
EUR 960.1

Genistin

GC2802-25 25
EUR 382.3

Genistin

GC2802-250 250
EUR 1845.7

Genistin

GC2802-50 50
EUR 577.8

Genistin

540154 500.0mg
EUR 450

Genistin

T2882-10mg 10mg Ask for price
Description: Genistin

Genistin

T2882-1g 1g Ask for price
Description: Genistin

Genistin

T2882-1mg 1mg Ask for price
Description: Genistin

Genistin

T2882-50mg 50mg Ask for price
Description: Genistin

Genistin

T2882-5mg 5mg Ask for price
Description: Genistin

Genistin

TB0370-1000 4X20mg
EUR 321.6

Genistin

HY-N0595 50mg
EUR 170.4

Genistin

N1860-20 20 mg
EUR 100
Description: TdT inhibitor

Genistin

abx282781-100g 100 µg Ask for price

Genistin

abx282781-20g 20 µg
EUR 106.25

Genistin

abx282781-50g 50 µg
EUR 250

A400, Type 1 Porous Strong Base Anion Resin, Chorlide Form

50218-1 1000ml
EUR 144
Description: 9052-45-3

A400, Type 1 Porous Strong Base Anion Resin, Chorlide Form

50218-250 250ml
EUR 47
Description: 9052-45-3

Genistin, >99%

BC034-025 25mg
EUR 278.4

Genistin, >99%

BC034-050 50mg
EUR 327.6

Genistin, >99%

BC034-100 100mg
EUR 537.6

Genistin(Genistoside)

E1KS2301 25mg
EUR 211.2

Genistin malonate

TB0588-0005 10mg
EUR 886.8

Optically clear sealing film for qPCR, Strong Bond, for PP plates, 100/pk

MS1000-PCR2 1 each
EUR 149.35

DiagAg™ Phenyl Agarose Strong Hydrophobic Medium, 6% Crosslinked, 24-45 µm

DAG-YS23-27 25 mL
EUR 850

Genistin 6''-O-Acetate

G350030 100mg
EUR 7600
Description: 73566-30-0

Genistin 6''-O-Malonate

G350035 250mg
EUR 11200
Description: 51011-05-3

Foil seals, strong bonding for cold storage (-200 to 110°C) and DMSO, for PP plates, 100/pk

MS1000-F2 1 each
EUR 149.35

DiagAg™ Phenyl Agarose Strong Hydrophobic Medium, 6% Crosslinked, 45-165 µm, Low Capacity

DAG-YS23-28 25 mL
EUR 850

DiagAg™ Phenyl Agarose Strong Hydrophobic Medium, 6% Crosslinked, 45-165 µm, High Capacity

DAG-YS23-29 25 mL
EUR 850

Rat Strongyloid IgG

QY-E11978 96T
EUR 420

genesig Easy kit for Strongylus vulgaris

Z-Path-S.vulgaris-EASY 50 tests
EUR 410
Description: S.vulgaris

Strongyloides IgG/IgM

DESTRO0690 96
EUR 134

Strongzyme Goat anti Mouse IgG (H+L) (HRP)

43R-1653 1 ml
EUR 763.2
Description: Strongzyme Goat anti-Mouse IgG (H+L) secondary antibody (HRP)

Strongzyme Rabbit anti Goat IgG (H+L) (HRP)

43R-1650 1 ml
EUR 813
Description: Strongzyme Rabbit anti Goat IgG (H + L) (HRP) secondary antibody

Strongzyme Goat anti Rabbit IgG (H + L) (HRP)

43R-1652 1 ml
EUR 648
Description: Strongzyme Goat anti Rabbit IgG (H + L) secondary antibody (HRP)

QPCR Kit DNA Strongylus vulgaris

MOL8942 EACH
EUR 987.17

Human Strongyloides IgG Elisa Kit

EK713927 96 Wells
EUR 0.4

QPCR Kit DNA Strongylus vulgaris - EACH

MOL8940 EACH
EUR 1129.51

genesig Real-time PCR detection kit for Strongylus vulgaris

Z-Path-S-vulgaris 150 tests
EUR 808
Description: S.vulgaris

genesig Std Real-time PCR detection kit, Strongylus vulgaris

Z-Path-S-vulgaris-std 150 tests
EUR 602
Description: S.vulgaris

Strongyloides (IgG/IgM) ELISA Kit (Human) (OKNA00174)

OKNA00174 96 Wells
EUR 505.2
Description: Description of target: Strongyloides is a genus containing some 50 species of obligate gastrointestinal parasites of vertebrates. Strongyloides stercoralis is the scientific name of a human parasitic roundworm causing the disease of strongyloidiasis. Its common name is pinworm in the UK and threadworm in the US. The Strongyloides stercoralis nematode can parasitize humans. The adult parasitic stage lives in tunnels in the mucosa of the small intestine.S. stercoralis can be found in areas with tropical and subtropical climates but cases also occur in temperate area, more frequently in rural areas. S. stercoralis has a very low prevalence in societies where fecal contamination of soil or water is rare. Many people infected are usually asymptomatic at first. Symptoms include dermatitis: swelling, itching, larva currens, and mild hemorrhage at the site where the skin has been penetrated. If the parasite reaches the lungs, the chest may feel as if it is burning, and wheezing and coughing may result, along with pneumonia-like symptoms (Löffler's syndrome). The intestines could eventually be invaded, leading to burning pain, tissue damage, sepsis, and ulcers. In severe cases, edema may result in obstruction of the intestinal tract, as well as loss of peristaltic contractions. Strongyloides infection in immunocompromised individuals (particularly following the administration of steroids, for example following transplant surgery) can result in disseminated strongyloidiasis, in which worms move beyond the confines of the gut into other organs. This is fatal unless antiStrongyloides therapy is given.Locating juvenile larvae, either rhabditiform or filariform, in recent stool samples will confirm the presence of this parasite. Other techniques used include direct fecal smears, culturing fecal samples on agar plates, serodiagnosis through ELISA, and duodenal fumigation.;Species reactivity: Human;Application: ;Assay info: Assay Methodology: Quantitative Reverse Capture Sandwich ELISA ;Sensitivity: Sensitivity is determined as the probability of the assay indicating a positive score in samples with the specific analyte present: 87.9%

StrongZyme Streptavidin-PolyHRP (High Load)

43R-1645 1 mg
EUR 380
Description: StrongZyme Streptavidin-PolyHRP conjugate (High Load)

Fscn1 (GFP-tagged) - Mouse fascin homolog 1, actin bundling protein (Strongylocentrotus purpuratus) (Fscn1)

MG207918 10 µg Ask for price

Fscn1 (untagged) - Mouse fascin homolog 1, actin bundling protein (Strongylocentrotus purpuratus) (Fscn1), (10ug)

MC216780 10 µg Ask for price

FSCN1 (GFP-tagged) - Human fascin homolog 1, actin-bundling protein (Strongylocentrotus purpuratus) (FSCN1)

RG203031 10 µg Ask for price

FSCN1 (untagged)-Human fascin homolog 1, actin-bundling protein (Strongylocentrotus purpuratus) (FSCN1)

SC118222 10 µg Ask for price

Fscn1 (Myc-DDK-tagged) - Mouse fascin homolog 1, actin bundling protein (Strongylocentrotus purpuratus) (Fscn1)

MR207918 10 µg Ask for price

FSCN1 (untagged)-Human fascin homolog 1, actin-bundling protein (Strongylocentrotus purpuratus) (FSCN1)

SC320744 10 µg Ask for price

Fscn2 (untagged) - Mouse fascin homolog 2, actin-bundling protein, retinal (Strongylocentrotus purpuratus) (Fscn2), (10ug)

MC216896 10 µg Ask for price

Fscn3 (untagged ORF) - Rat fascin homolog 3, actin-bundling protein, testicular (Strongylocentrotus purpuratus) (Fscn3), (10 ug)

RN201308 10 µg Ask for price

Rabbit polyclonal antibody to Fascin (fascin homolog 1, actin-bundling protein (Strongylocentrotus purpuratus))

TA308801 100 µl Ask for price

FSCN1 (Myc-DDK-tagged)-Human fascin homolog 1, actin-bundling protein (Strongylocentrotus purpuratus) (FSCN1)

RC203031 10 µg Ask for price

Lenti ORF clone of Human fascin homolog 1, actin-bundling protein (Strongylocentrotus purpuratus) (FSCN1), mGFP tagged

RC203031L2 10 µg Ask for price

Lenti ORF clone of Human fascin homolog 1, actin-bundling protein (Strongylocentrotus purpuratus) (FSCN1), mGFP tagged

RC203031L4 10 µg Ask for price

Lenti ORF clone of Fscn1 (mGFP-tagged) - Mouse fascin homolog 1, actin bundling protein (Strongylocentrotus purpuratus) (Fscn1)

MR207918L4 10 µg Ask for price

Fscn2 (untagged ORF) - Rat fascin homolog 2, actin-bundling protein, retinal (Strongylocentrotus purpuratus) (Fscn2), (10 ug)

RN206219 10 µg Ask for price

Fscn3 (GFP-tagged) - Mouse fascin homolog 3, actin-bundling protein, testicular (Strongylocentrotus purpuratus) (Fscn3)

MG207995 10 µg Ask for price

Fscn3 (untagged) - Mouse fascin homolog 3, actin-bundling protein, testicular (Strongylocentrotus purpuratus) (Fscn3), (10ug)

MC216995 10 µg Ask for price

FSCN3 (GFP-tagged) - Human fascin homolog 3, actin-bundling protein, testicular (Strongylocentrotus purpuratus) (FSCN3)

RG207492 10 µg Ask for price

Fscn2 (Myc-DDK-tagged ORF) - Rat fascin homolog 2, actin-bundling protein, retinal (Strongylocentrotus purpuratus) (Fscn2), (10 ug)

RR206219 10 µg Ask for price

Fscn3 (Myc-DDK-tagged ORF) - Rat fascin homolog 3, actin-bundling protein, testicular (Strongylocentrotus purpuratus) (Fscn3), (10 ug)

RR201308 10 µg Ask for price

3`UTR clone of fascin homolog 1 actin-bundling protein (Strongylocentrotus purpuratus) (FSCN1) for miRNA target validation

SC213307 10 µg Ask for price

FSCN3 (untagged)-Human fascin homolog 3, actin-bundling protein, testicular (Strongylocentrotus purpuratus) (FSCN3)

SC323970 10 µg Ask for price

Lenti ORF clone of Human fascin homolog 1, actin-bundling protein (Strongylocentrotus purpuratus) (FSCN1), Myc-DDK-tagged

RC203031L1 10 µg Ask for price

Lenti ORF clone of Human fascin homolog 1, actin-bundling protein (Strongylocentrotus purpuratus) (FSCN1), Myc-DDK-tagged

RC203031L3 10 µg Ask for price

Fscn2 (Myc-DDK-tagged) - Mouse fascin homolog 2, actin-bundling protein, retinal (Strongylocentrotus purpuratus) (Fscn2)

MR225952 10 µg Ask for price

Lenti ORF clone of Fscn1 (Myc-DDK-tagged) - Mouse fascin homolog 1, actin bundling protein (Strongylocentrotus purpuratus) (Fscn1)

MR207918L3 10 µg Ask for price

Fscn2 (GFP-tagged) - Mouse fascin homolog 2 actin-bundling protein retinal (Strongylocentrotus purpuratus) (Fscn2), (10ug)

MG225952 10 µg Ask for price

FSCN3 (Myc-DDK-tagged)-Human fascin homolog 3, actin-bundling protein, testicular (Strongylocentrotus purpuratus) (FSCN3)

RC207492 10 µg Ask for price

Lenti ORF clone of Human fascin homolog 3, actin-bundling protein, testicular (Strongylocentrotus purpuratus) (FSCN3), mGFP tagged

RC207492L4 10 µg Ask for price

Lenti ORF clone of Fscn2 (Myc-DDK-tagged ORF) - Rat fascin homolog 2, actin-bundling protein, retinal (Strongylocentrotus purpuratus) (Fscn2), (1

RR206219L3 10 µg Ask for price

Lenti ORF clone of Fscn2 (mGFP-tagged ORF) - Rat fascin homolog 2, actin-bundling protein, retinal (Strongylocentrotus purpuratus) (Fscn2), (10 u

RR206219L4 10 µg Ask for price

Lenti ORF particles, FSCN1 (Myc-DDK tagged) - Human fascin homolog 1, actin-bundling protein (Strongylocentrotus purpuratus) (FSCN1), 200ul, >10^

RC203031L1V 200 µl Ask for price

Lenti ORF particles, FSCN1 (mGFP-tagged) - Human fascin homolog 1, actin-bundling protein (Strongylocentrotus purpuratus) (FSCN1), 200ul, >10^7 T

RC203031L2V 200 µl Ask for price

Lenti ORF particles, FSCN1 (Myc-DDK tagged) - Human fascin homolog 1, actin-bundling protein (Strongylocentrotus purpuratus) (FSCN1), 200ul, >10^

RC203031L3V 200 µl Ask for price

Lenti ORF particles, FSCN1 (mGFP-tagged) - Human fascin homolog 1, actin-bundling protein (Strongylocentrotus purpuratus) (FSCN1), 200ul, >10^7 T

RC203031L4V 200 µl Ask for price

Lenti ORF clone of Fscn2 (mGFP-tagged) - Mouse fascin homolog 2, actin-bundling protein, retinal (Strongylocentrotus purpuratus) (Fscn2)

MR225952L4 10 µg Ask for price

Lenti ORF particles, Fscn1 (Myc-DDK-tagged) - Mouse fascin homolog 1, actin bundling protein (Strongylocentrotus purpuratus) (Fscn1), 200ul, >10^

MR207918L3V 200 µl Ask for price

Lenti ORF particles, Fscn1 (GFP-tagged) - Mouse fascin homolog 1, actin bundling protein (Strongylocentrotus purpuratus) (Fscn1), 200ul, >10^7 TU

MR207918L4V 200 µl Ask for price

Fscn3 (Myc-DDK-tagged) - Mouse fascin homolog 3, actin-bundling protein, testicular (Strongylocentrotus purpuratus) (Fscn3)

MR207995 10 µg Ask for price

Lenti ORF particles, FSCN2 (Myc-DDK tagged) - Human fascin homolog 2, actin-bundling protein, retinal (Strongylocentrotus purpuratus) (FSCN2), tr

RC221223L3V 200 µl Ask for price

Lenti ORF clone of Human fascin homolog 2, actin-bundling protein, retinal (Strongylocentrotus purpuratus) (FSCN2), transcript variant 1, mGFP ta

RC221223L4 10 µg Ask for price

Lenti ORF particles, FSCN2 (Myc-DDK tagged) - Human fascin homolog 2, actin-bundling protein, retinal (Strongylocentrotus purpuratus) (FSCN2), tr

RC221276L3V 200 µl Ask for price

Lenti ORF clone of Human fascin homolog 2, actin-bundling protein, retinal (Strongylocentrotus purpuratus) (FSCN2), transcript variant 2, mGFP ta

RC221276L4 10 µg Ask for price

Lenti ORF clone of Human fascin homolog 3, actin-bundling protein, testicular (Strongylocentrotus purpuratus) (FSCN3), Myc-DDK-tagged

RC207492L3 10 µg Ask for price

FSCN2 (GFP-tagged) - Human fascin homolog 2, actin-bundling protein, retinal (Strongylocentrotus purpuratus) (FSCN2), transcript variant 1

RG221223 10 µg Ask for price

FSCN2 (GFP-tagged) - Human fascin homolog 2, actin-bundling protein, retinal (Strongylocentrotus purpuratus) (FSCN2), transcript variant 2

RG221276 10 µg Ask for price

Lenti ORF particles, Fscn2 (Myc-DDK-tagged ORF) - Rat fascin homolog 2, actin-bundling protein, retinal (Strongylocentrotus purpuratus) (Fscn2),

RR206219L3V 200 µl Ask for price

Lenti ORF particles, Fscn2 (GFP-tagged ORF) - Rat fascin homolog 2, actin-bundling protein, retinal (Strongylocentrotus purpuratus) (Fscn2), 200u

RR206219L4V 200 µl Ask for price

Lenti ORF clone of Fscn3 (Myc-DDK-tagged ORF) - Rat fascin homolog 3, actin-bundling protein, testicular (Strongylocentrotus purpuratus) (Fscn3),

RR201308L3 10 µg Ask for price

Lenti ORF clone of Fscn3 (mGFP-tagged ORF) - Rat fascin homolog 3, actin-bundling protein, testicular (Strongylocentrotus purpuratus) (Fscn3), (1

RR201308L4 10 µg Ask for price

Lenti ORF particles, Fscn3 (GFP-tagged ORF) - Rat fascin homolog 3, actin-bundling protein, testicular (Strongylocentrotus purpuratus) (Fscn3), 2

RR201308L4V 200 µl Ask for price

FSCN2 (untagged)-Human fascin homolog 2, actin-bundling protein, retinal (Strongylocentrotus purpuratus) (FSCN2), transcript variant 1

SC303980 10 µg Ask for price

Lenti ORF clone of Fscn2 (Myc-DDK-tagged) - Mouse fascin homolog 2, actin-bundling protein, retinal (Strongylocentrotus purpuratus) (Fscn2)

MR225952L3 10 µg Ask for price

Lenti ORF particles, Fscn2 (GFP-tagged) - Mouse fascin homolog 2, actin-bundling protein, retinal (Strongylocentrotus purpuratus) (Fscn2), 200ul,

MR225952L4V 200 µl Ask for price

Lenti ORF clone of Fscn3 (Myc-DDK-tagged) - Mouse fascin homolog 3, actin-bundling protein, testicular (Strongylocentrotus purpuratus) (Fscn3)

MR207995L3 10 µg Ask for price

Lenti ORF clone of Fscn3 (mGFP-tagged) - Mouse fascin homolog 3, actin-bundling protein, testicular (Strongylocentrotus purpuratus) (Fscn3)

MR207995L4 10 µg Ask for price

FSCN2 (untagged)-Human fascin homolog 2, actin-bundling protein, retinal (Strongylocentrotus purpuratus) (FSCN2), transcript variant 2

SC315582 10 µg Ask for price

FSCN2 (Myc-DDK-tagged)-Human fascin homolog 2, actin-bundling protein, retinal (Strongylocentrotus purpuratus) (FSCN2), transcript variant 1

RC221223 10 µg Ask for price

Lenti ORF clone of Human fascin homolog 2, actin-bundling protein, retinal (Strongylocentrotus purpuratus) (FSCN2), transcript variant 1, Myc-DDK

RC221223L3 10 µg Ask for price

Lenti ORF particles, FSCN2 (mGFP-tagged) - Human fascin homolog 2, actin-bundling protein, retinal (Strongylocentrotus purpuratus) (FSCN2), trans

RC221223L4V 200 µl Ask for price

FSCN2 (Myc-DDK-tagged)-Human fascin homolog 2, actin-bundling protein, retinal (Strongylocentrotus purpuratus) (FSCN2), transcript variant 2

RC221276 10 µg Ask for price

Lenti ORF clone of Human fascin homolog 2, actin-bundling protein, retinal (Strongylocentrotus purpuratus) (FSCN2), transcript variant 2, Myc-DDK

RC221276L3 10 µg Ask for price

Lenti ORF particles, FSCN2 (mGFP-tagged) - Human fascin homolog 2, actin-bundling protein, retinal (Strongylocentrotus purpuratus) (FSCN2), trans

RC221276L4V 200 µl Ask for price

Lenti ORF particles, FSCN3 (Myc-DDK tagged) - Human fascin homolog 3, actin-bundling protein, testicular (Strongylocentrotus purpuratus) (FSCN3),

RC207492L3V 200 µl Ask for price

Lenti ORF particles, FSCN3 (mGFP-tagged) - Human fascin homolog 3, actin-bundling protein, testicular (Strongylocentrotus purpuratus) (FSCN3), 20

RC207492L4V 200 µl Ask for price

Lenti ORF particles, Fscn3 (Myc-DDK-tagged ORF) - Rat fascin homolog 3, actin-bundling protein, testicular (Strongylocentrotus purpuratus) (Fscn3

RR201308L3V 200 µl Ask for price

Lenti ORF particles, Fscn2 (Myc-DDK-tagged) - Mouse fascin homolog 2, actin-bundling protein, retinal (Strongylocentrotus purpuratus) (Fscn2), 20

MR225952L3V 200 µl Ask for price

Lenti ORF particles, Fscn3 (Myc-DDK-tagged) - Mouse fascin homolog 3, actin-bundling protein, testicular (Strongylocentrotus purpuratus) (Fscn3),

MR207995L3V 200 µl Ask for price

Lenti ORF particles, Fscn3 (GFP-tagged) - Mouse fascin homolog 3, actin-bundling protein, testicular (Strongylocentrotus purpuratus) (Fscn3), 200

MR207995L4V 200 µl Ask for price

Human Strontium (Strontium) Elisa Kit

EK710512 96 Wells
EUR 0.98

Strontium Oxide

32315-42 25G
EUR 61.6

Strontium Oxide

abx186564-500g 500 g
EUR 309.6

Strontium oxide, 99.5%

GX8751-50 50
EUR 585.6

Strontium Iodate 99%

S09620 10G
EUR 135

Strontium Acetate

32302-02 25G
EUR 22.75

Strontium acetate

S09490 100G
EUR 216.3

Strontium bromide 99%

S09540 100G
EUR 242.2

Strontium ferrite 99.5%

S09575 5G
EUR 191.06

Strontium formate

S09590 25G
EUR 253.25

Strontium hydride 99.5%

S09600 1G
EUR 188.28

Strontium lactate

S09650 100G
EUR 1278.7

Strontium oxalate

S09697 100G
EUR 99.5

Strontium sulfate

S09810 25G
EUR 106.53

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