Efficient detection of eukaryotic calcium-sensing receptor

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In direction of middle-up evaluation of polyclonal antibodies: subclass-specific N-glycosylation profiling of murine immunoglobulin G (IgG) by the use of HPLC-MS

 

  • Lately, superior HPLC-MS methods based mostly on intact protein (“top-down”) or protein subunit (“middle-up/middle-down”) evaluation have been applied for the characterization of therapeutic monoclonal antibodies. Right here, we assess feasibility of middle-up/middle-down evaluation for polyclonal IgGs exhibiting in depth sequence variability. Particularly, we addressed IgGs from mouse, representing an essential mannequin system in immunological investigations.
  • To acquire Fc/2 parts as conserved subunits of IgGs, we made use of the bacterial protease SpeB. For this function, we initially decided SpeB cleavage websites in murine IgGs. The ensuing Fc/2 parts attribute of various subclasses had been subsequently analysed by ion-pair reversed-phase HPLC hyphenated to high-resolution mass spectrometry. This enabled simultaneous relative quantification of IgG subclasses and their N-glycosylation variants, each of which affect IgG effector features.
  • To evaluate methodology capabilities in an immunological context, we utilized the analytical workflow to polyclonal antibodies obtained from BALB/c mice immunized with the grass pollen allergen Phl p 6. The research revealed a shift in IgG subclasses and Fc-glycosylation patterns in whole and antigen-specific IgGs from totally different mouse cohorts, respectively. Finally, Fc/2 characterization might reveal different protein modifications together with oxidation, amino acid exchanges, and C-terminal lysine, and will thus be applied for high quality management of practical antibodies.

Environment friendly detection of eukaryotic calcium-sensing receptor (CaSR) by polyclonal antibody towards prokaryotic expressed truncated CaSR

 

Calcium-sensing receptor (CaSR), which is best identified for its motion as regulating calcium homeostasis, can bind varied ligands. To facilitate analysis on CaSR and perceive the receptor’s operate additional, an in silico designed truncated protein was developed. The ensuing protein folding indicated that 99% of predicted three dimensional (3D) construction residues are positioned in favored and allowed Ramachandran plots. Nonetheless, it was discovered that such protein doesn’t fold correctly when expressed in prokaryotic host cells.

Thioredoxin (Trx) tag was conjugated to extend the ultimate protein’s solubility, which may assist acquire the soluble antigen with higher immunogenic properties. The truncated recombinant proteins had been expressed and purified in two kinds (Trx-CaSR: RR19 and CaSR: RRJ19). The polyclonal antibody was induced by the rabbit immunization with the type of RR19. Western blot on mouse kidney lysates evidenced the correct immune recognition of the receptor by the produced antibody.

The specificity and sensitivity of antibodies had been additionally assayed by immunohistofluorescence. These experiments affirmed antibody’s means to point the receptor on the cell floor in native type and the potential for making use of such antibodies in additional mobile and tissue assays.

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Doxorubicin hydrochloride

GA4969-100MG 100 mg
EUR 217.2

Doxorubicin hydrochloride

GA4969-10MG 10 mg
EUR 93.6

Doxorubicin hydrochloride

GA4969-25MG 25 mg
EUR 117.6

Doxorubicin hydrochloride

GA4969-50MG 50 mg
EUR 151.2

Doxorubicin (hydrochloride)

HY-15142 100mg
EUR 176.4

Doxorubicin hydrochloride

TNC00267 20mg Ask for price

Doxorubicin hydrochloride, 98%

GP9056-100 100
EUR 140.2

Doxorubicin hydrochloride, 98%

GP9056-25 25
EUR 56.1

Doxorubicin hydrochloride USP

D005-10MG 10 mg
EUR 199.2

Doxorubicin hydrochloride USP

D005-25MG 25 mg
EUR 361.2

Doxorubicin hydrochloride, USP grade

GA4969-10 10
EUR 47.4

Doxorubicin hydrochloride, USP grade

GA4969-100 100
EUR 150.4

Doxorubicin hydrochloride, USP grade

GA4969-25 25
EUR 67.2

Doxorubicin hydrochloride, USP grade

GA4969-50 50
EUR 95

Doxorubicin Hydrochloride (Autophagy inducer)

SIH-390-10MG 10 mg
EUR 231.6
Description: The substance Doxorubicin Hydrochloride is a autophagy inducer. It is synthetically produced and has a purity of >98%. The pure substance is red solid which is soluble in water (50 mM)..

Doxorubicin Hydrochloride (Autophagy inducer)

SIH-390-50MG 50 mg
EUR 634.8
Description: The substance Doxorubicin Hydrochloride is a autophagy inducer. It is synthetically produced and has a purity of >98%. The pure substance is red solid which is soluble in water (50 mM)..

Doxorubicin

A3966-10 10 mg
EUR 129.6
Description: Doxorubicin is a semi-synthesized anticancer agent derived from bacterial culture. [1] It is an anthracycline antibiotic. It is been widely used in blood cancers, solid tumors and sarcomas.

Doxorubicin

A3966-100 100 mg
EUR 282
Description: Doxorubicin is a semi-synthesized anticancer agent derived from bacterial culture. [1] It is an anthracycline antibiotic. It is been widely used in blood cancers, solid tumors and sarcomas.

Doxorubicin

A3966-25 25 mg
EUR 157.2
Description: Doxorubicin is a semi-synthesized anticancer agent derived from bacterial culture. [1] It is an anthracycline antibiotic. It is been widely used in blood cancers, solid tumors and sarcomas.

Doxorubicin

A3966-5.1 10 mM (in 1mL DMSO)
EUR 157.2
Description: Doxorubicin is a semi-synthesized anticancer agent derived from bacterial culture. [1] It is an anthracycline antibiotic. It is been widely used in blood cancers, solid tumors and sarcomas.

Doxorubicin

A3966-S Evaluation Sample
EUR 97.2
Description: Doxorubicin is a semi-synthesized anticancer agent derived from bacterial culture. [1] It is an anthracycline antibiotic. It is been widely used in blood cancers, solid tumors and sarcomas.

Doxorubicin

ADC-P-013 unit Ask for price

Doxorubicin

abx188695-1g 1 g
EUR 560.4

Doxorubicin

D063-100MG 100 mg
EUR 1261.2

Doxorubicin

D063-25MG 25 mg
EUR 386.4

Doxorubicin

HY-15142A 100mg
EUR 258

Doxorubicin HCl

20-abx184016
  • EUR 895.20
  • EUR 1779.60
  • 1 g
  • 5 g

Doxorubicin 10mM

TG4120 0.25ml
EUR 187.2

Doxorubicin HCl, 100mg

R2531-100mg each
EUR 1560

Doxorubicin HCl, 10mg

R2531-10mg each
EUR 236.4

Adriamycin (Doxorubicin)

E1KS1208 10mg
EUR 490.8

Doxorubicin (Adriamycin) HCl

A1832-10 10 mg
EUR 150
Description: Doxorubicin is a semi-synthesized anticancer agent derived from bacterial culture. [1] It is an anthracycline antibiotic. It is been widely used in blood cancers, solid tumors and sarcomas.

Doxorubicin (Adriamycin) HCl

A1832-100 100 mg
EUR 560.4
Description: Doxorubicin is a semi-synthesized anticancer agent derived from bacterial culture. [1] It is an anthracycline antibiotic. It is been widely used in blood cancers, solid tumors and sarcomas.

Doxorubicin (Adriamycin) HCl

A1832-25 25 mg
EUR 226.8
Description: Doxorubicin is a semi-synthesized anticancer agent derived from bacterial culture. [1] It is an anthracycline antibiotic. It is been widely used in blood cancers, solid tumors and sarcomas.

Doxorubicin (Adriamycin) HCl

A1832-5.1 10 mM (in 1mL DMSO)
EUR 135.6
Description: Doxorubicin is a semi-synthesized anticancer agent derived from bacterial culture. [1] It is an anthracycline antibiotic. It is been widely used in blood cancers, solid tumors and sarcomas.

PEGylated Liposomal Doxorubicin- 2 mg

PHPC002DX 2 mg
EUR 140.62
Description: This product includes 1 ml of liposomal doxorubicin at concentrations of 2 mg/ml doxorubicin and 16 mg/ml of lipids.

PEGylated Liposomal Doxorubicin- 20 mg

PHPC020DX 20 mg
EUR 358.62
Description: This product includes 10 ml of liposomal doxorubicin at concentrations of 2 mg/ml doxorubicin and 16 mg/ml of lipids.

Erlotinib, Hydrochloride Salt

1588-100 each
EUR 202.8

Erlotinib, Hydrochloride Salt

1588-1000 each
EUR 477.6

Erlotinib, Hydrochloride Salt, >99%

BC026-101 1g
EUR 327.6

Erlotinib, Hydrochloride Salt, >99%

BC026-102 2g
EUR 465.6

Erlotinib, Hydrochloride Salt, >99%

BC026-105 5g
EUR 684

17-DMAG, Hydrochloride Salt, >98%

BC024-001 1mg
EUR 213.6

17-DMAG, Hydrochloride Salt, >98%

BC024-005 5mg
EUR 343.2

17-DMAG, Hydrochloride Salt, >98%

BC024-010 10mg
EUR 489.6

17-DMAG, Hydrochloride Salt, >98%

BC024-025 25mg Ask for price

17-DMAG, Hydrochloride Salt, >99%

BC024-100 100mg Ask for price

17-DMAG, Hydrochloride Salt, >99%

BC024-101 1g Ask for price

17-DMAG, Hydrochloride Salt, >99%

BC024-250 250mg Ask for price

OSU-03012, Hydrochloride Salt, >98%

BC061-005 5mg
EUR 375.6

OSU-03012, Hydrochloride Salt, >98%

BC061-025 25mg
EUR 634.8

Human HeLa (Cervix Adenocarcinoma) Whole Cell Lysate - Doxorubicin Stimulated

HCL-2010 100ug
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Human HeLa (Cervix Adenocarcinoma) Cell Nuclear Extract - Doxorubicin Stimulated

HCL-2004 100ug
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Doxorubicin.HCl

1527-5 each
EUR 183.6

L-Leucine 7-amido-4-methylcoumarin hydrochloride salt

GC8248-100 100
EUR 143.7

L-Leucine 7-amido-4-methylcoumarin hydrochloride salt

GC8248-25 25
EUR 57.8

Doxepin (hydrochloride)

C4153-5.1 10 mM (in 1mL DMSO)
EUR 135.6
Description: Doxepin, a tricyclic antidepressant, is a potent histamine H1 antagonist [1]. The histamine receptors belong to a family of G protein?coupled receptors activated by their primary endogenous ligand histamine.

Doxepin (hydrochloride)

C4153-5000 5 g
EUR 201.6
Description: Doxepin, a tricyclic antidepressant, is a potent histamine H1 antagonist [1]. The histamine receptors belong to a family of G protein?coupled receptors activated by their primary endogenous ligand histamine.

Doxepin (Hydrochloride)

HY-B0725 500mg
EUR 129.6

Doxapram hydrochloride

GP6685-100 100
EUR 201.9

Doxycycline Hydrochloride

D065-25MG 25mg
EUR 410.4

Doxycycline Hydrochloride

D065-5MG 5mg
EUR 142.8

Doxycycline hydrochloride

GA4252-1G 1 g
EUR 99.6

Doxycycline hydrochloride

GA4252-500MG 500 mg
EUR 74.4

Doxycycline (hydrochloride)

HY-N0565A 1g
EUR 142.8

Doxycycline hydrochloride

GA4252-1 1
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Doxycycline hydrochloride

GA4252-500 500
EUR 31.7

Doxapram (hydrochloride hydrate)

HY-B0551A 10mM/1mL
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(±)-Salsolinol (hydrochloride)

C4364-500 500 mg
EUR 369.6
Description: (±)-Salsolinol targets depolarize dopamineric neurons. Dopaminergic neurons produce dopamine, a neurotransmitter with various roles in neurological functions.

2‐AMINO ACETAMIDE HYDROCHLORIDE (GLYCINAMIDE HYDROCHLORIDE)

101003 each Ask for price

DOI hydrochloride

B5321-10 10 mg
EUR 205.2

DOI hydrochloride

B5321-5 5 mg
EUR 141.6

DOI hydrochloride

B5321-50 50 mg
EUR 714

DOB hydrochloride

B5344-1 1 mg
EUR 141.6

Q94 hydrochloride

B5705-10 10 mg
EUR 350.4

Q94 hydrochloride

B5705-50 50 mg
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GMQ hydrochloride

B5793-10 10 mg
EUR 212.4

GMQ hydrochloride

B5793-50 50 mg
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AMT hydrochloride

B6480-10 10 mg
EUR 205.2

AMT hydrochloride

B6480-100 100 mg
EUR 1036.8

AMT hydrochloride

B6480-5 5 mg
EUR 141.6

AMT hydrochloride

B6480-50 50 mg
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EDC hydrochloride

GP0849-100G 100 g
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EDC hydrochloride

GP0849-250G 250 g
EUR 237.6

EDC hydrochloride

GP0849-25G 25 g
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EDC hydrochloride

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EUR 55.2

SAG (hydrochloride)

HY-12848B 10mg
EUR 321.6

BEC (hydrochloride)

HY-19548A 25mg
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EDC hydrochloride

GP0849-100 100
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EDC hydrochloride

GP0849-25 25
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EDC hydrochloride

GP0849-250 250
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EDC hydrochloride

GP0849-5 5
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EHNA hydrochloride

2265-25 each
EUR 339.6

EHNA hydrochloride

2265-5 each
EUR 138

MPEP Hydrochloride

A3633-10 10 mg
EUR 142.8
Description: IC50: 36 nMMPEP is a potent, selective and systemically active mGlu5 receptor antagonist. Metabotropic glutamate (mGlu) receptors are a of G-protein-coupled receptor family linked to multiple second messengers and modulation of ion channel functions in the nervous system.

MPEP Hydrochloride

A3633-50 50 mg
EUR 379.2
Description: IC50: 36 nMMPEP is a potent, selective and systemically active mGlu5 receptor antagonist. Metabotropic glutamate (mGlu) receptors are a of G-protein-coupled receptor family linked to multiple second messengers and modulation of ion channel functions in the nervous system.

MPTP hydrochloride

A3634-10 10 mg
EUR 162
Description: IC50 Value: 53uM?inhibited the nerve-evoked twitches of phrenic nerve-hemidiaphragm preparations from ICR mice? . MPTP HCl (Sigma-Chem.) induced reduction in the DOPAC HVA/dopamine (DA) ratio and increase in striatal ascorbate (AS) oxidation in rats [1].

MPTP hydrochloride

A3634-50 50 mg
EUR 344.4
Description: IC50 Value: 53uM?inhibited the nerve-evoked twitches of phrenic nerve-hemidiaphragm preparations from ICR mice? . MPTP HCl (Sigma-Chem.) induced reduction in the DOPAC HVA/dopamine (DA) ratio and increase in striatal ascorbate (AS) oxidation in rats [1].

MTEP hydrochloride

A3636-10 10 mg
EUR 205.2
Description: IC50: 5 nMMTEP is a selective metabotropic glutamate receptor subtype 5 (mGluR5) antagonist.The mGluRs are classified into three groups: group I (mGluR1 and 5), group II (mGluR2 and 3) and group III (mGluR4, 6, 7 and 8).

MTEP hydrochloride

A3636-5 5 mg
EUR 153.6
Description: IC50: 5 nMMTEP is a selective metabotropic glutamate receptor subtype 5 (mGluR5) antagonist.The mGluRs are classified into three groups: group I (mGluR1 and 5), group II (mGluR2 and 3) and group III (mGluR4, 6, 7 and 8).

MTEP hydrochloride

A3636-50 50 mg
EUR 686.4
Description: IC50: 5 nMMTEP is a selective metabotropic glutamate receptor subtype 5 (mGluR5) antagonist.The mGluRs are classified into three groups: group I (mGluR1 and 5), group II (mGluR2 and 3) and group III (mGluR4, 6, 7 and 8).

PJ34 hydrochloride

A4159-10 10 mg
EUR 142.8
Description: PJ34 is a novel and potent inhibitor of poly(ADP-ribose) polymerase (PARP), an enzyme involved in DNA repair and cell proliferation, that dose-dependently inhibits purified PARP enzyme in a cell-free assay with half maximal effective concentration EC50 value of 20 nM.

PJ34 hydrochloride

A4159-5 5 mg
EUR 129.6
Description: PJ34 is a novel and potent inhibitor of poly(ADP-ribose) polymerase (PARP), an enzyme involved in DNA repair and cell proliferation, that dose-dependently inhibits purified PARP enzyme in a cell-free assay with half maximal effective concentration EC50 value of 20 nM.

PJ34 hydrochloride

A4159-5.1 10 mM (in 1mL DMSO)
EUR 150
Description: PJ34 is a novel and potent inhibitor of poly(ADP-ribose) polymerase (PARP), an enzyme involved in DNA repair and cell proliferation, that dose-dependently inhibits purified PARP enzyme in a cell-free assay with half maximal effective concentration EC50 value of 20 nM.

PJ34 hydrochloride

A4159-50 50 mg
EUR 296.4
Description: PJ34 is a novel and potent inhibitor of poly(ADP-ribose) polymerase (PARP), an enzyme involved in DNA repair and cell proliferation, that dose-dependently inhibits purified PARP enzyme in a cell-free assay with half maximal effective concentration EC50 value of 20 nM.

PJ34 hydrochloride

A4159-S Evaluation Sample
EUR 97.2
Description: PJ34 is a novel and potent inhibitor of poly(ADP-ribose) polymerase (PARP), an enzyme involved in DNA repair and cell proliferation, that dose-dependently inhibits purified PARP enzyme in a cell-free assay with half maximal effective concentration EC50 value of 20 nM.

THZ1 hydrochloride

9544-25 each
EUR 1227.6

THZ1 hydrochloride

9544-5 each
EUR 366

MPEP hydrochloride

B1151-10 each
EUR 222

MPEP hydrochloride

B1151-50 each
EUR 705.6

S1RA hydrochloride

B1180-10 10 mg
EUR 1144.8
Description: S1RA hydrochloride is a potent and selective antagonist of ?1 receptor (?1R) with Ki value of 17nM [1].S1RA is the first ?1 receptor antagonist with potent antinociceptive activities in various pain models.

S1RA hydrochloride

B1180-100 100 mg
EUR 4620
Description: S1RA hydrochloride is a potent and selective antagonist of ?1 receptor (?1R) with Ki value of 17nM [1].S1RA is the first ?1 receptor antagonist with potent antinociceptive activities in various pain models.

S1RA hydrochloride

B1180-5 5 mg
EUR 819.6
Description: S1RA hydrochloride is a potent and selective antagonist of ?1 receptor (?1R) with Ki value of 17nM [1].S1RA is the first ?1 receptor antagonist with potent antinociceptive activities in various pain models.

S1RA hydrochloride

B1180-5.1 10 mM (in 1mL DMSO)
EUR 895.2
Description: S1RA hydrochloride is a potent and selective antagonist of ?1 receptor (?1R) with Ki value of 17nM [1].S1RA is the first ?1 receptor antagonist with potent antinociceptive activities in various pain models.

S1RA hydrochloride

B1180-50 50 mg
EUR 3316.8
Description: S1RA hydrochloride is a potent and selective antagonist of ?1 receptor (?1R) with Ki value of 17nM [1].S1RA is the first ?1 receptor antagonist with potent antinociceptive activities in various pain models.

MMAD hydrochloride

ADC-P-019 unit Ask for price

MMAF Hydrochloride

ADC-P-021 unit Ask for price

TAME Hydrochloride

2050-100 each
EUR 151.2

TAME Hydrochloride

2050-1000 each
EUR 574.8

TAME Hydrochloride

2050-500 each
EUR 405.6

THZ1 Hydrochloride

B4736-10 10 mg
EUR 268.8
Description: THZ1 is a covalent inhibitor of CDK7 with IC50 value of 3.2nM [1].THZ1 covalently modifies CDK7 by targeting C312 residue outside of the kinase domain, providing an unanticipated means of achieving covalent selectivity.

THZ1 Hydrochloride

B4736-25 25 mg
EUR 477.6
Description: THZ1 is a covalent inhibitor of CDK7 with IC50 value of 3.2nM [1].THZ1 covalently modifies CDK7 by targeting C312 residue outside of the kinase domain, providing an unanticipated means of achieving covalent selectivity.

THZ1 Hydrochloride

B4736-5 5 mg
EUR 170.4
Description: THZ1 is a covalent inhibitor of CDK7 with IC50 value of 3.2nM [1].THZ1 covalently modifies CDK7 by targeting C312 residue outside of the kinase domain, providing an unanticipated means of achieving covalent selectivity.

THZ1 Hydrochloride

B4736-5.1 10 mM (in 1mL DMSO)
EUR 205.2
Description: THZ1 is a covalent inhibitor of CDK7 with IC50 value of 3.2nM [1].THZ1 covalently modifies CDK7 by targeting C312 residue outside of the kinase domain, providing an unanticipated means of achieving covalent selectivity.

TCEP hydrochloride

B6055-10000 10 g
EUR 338.4
Description: Tris(2-carboxyethyl)phosphine hydrochloride (TCEP HCL) is a water soluble strong reducing agent that cleave disulfide bonds. It is a non-thiol and non-volatile solid.

TCEP hydrochloride

B6055-2000 2 g
EUR 150
Description: Tris(2-carboxyethyl)phosphine hydrochloride (TCEP HCL) is a water soluble strong reducing agent that cleave disulfide bonds. It is a non-thiol and non-volatile solid.

TCEP hydrochloride

B6055-50000 50 g
EUR 1243.2
Description: Tris(2-carboxyethyl)phosphine hydrochloride (TCEP HCL) is a water soluble strong reducing agent that cleave disulfide bonds. It is a non-thiol and non-volatile solid.

HEAT hydrochloride

B6339-10 10 mg
EUR 321.6

HEAT hydrochloride

B6339-50 50 mg
EUR 1167.6

THIP hydrochloride

B6460-100 100 mg
EUR 358.8

THIP hydrochloride

B6460-25 25 mg
EUR 174

THIP hydrochloride

B6460-50 50 mg
EUR 243.6

AMTB hydrochloride

B2978-25 25 mg
EUR 812.4

AMTB hydrochloride

B2978-5 5 mg
EUR 247.2

DMCM hydrochloride

B7268-10 10 mg
EUR 385.2

DMCM hydrochloride

B7268-100 100 mg
EUR 2210.4

DMCM hydrochloride

B7268-25 25 mg
EUR 776.4

DMCM hydrochloride

B7268-5 5 mg
EUR 277.2

DMCM hydrochloride

B7268-5.1 10 mM (in 1mL H2O)
EUR 297.6

DMCM hydrochloride

B7268-50 50 mg
EUR 1363.2

TRIS hydrochloride

B7299-500000 500 g
EUR 309.6

AMTB hydrochloride

B7559-10 10 mg
EUR 459.6

AMTB hydrochloride

B7559-50 50 mg
EUR 1746

EHNA hydrochloride

B6662-10 10 mg
EUR 145.2

EHNA hydrochloride

B6662-25 25 mg
EUR 262.8

N20C hydrochloride

B6980-10 10 mg
EUR 340.8

N20C hydrochloride

B6980-50 50 mg
EUR 1245.6

TMPH hydrochloride

B7053-10 10 mg
EUR 321.6

TMPH hydrochloride

B7053-50 50 mg
EUR 1167.6

DAPI (hydrochloride)

C3362-10 10 mg
EUR 146.4

DAPI (hydrochloride)

C3362-25 25 mg
EUR 240

DAPI (hydrochloride)

C3362-5 5 mg
EUR 111.6

AD57 (hydrochloride)

C3080-1 1 mg
EUR 141.6
Description: IC50: 2 nM: blocks the receptor tyrosine kinase RET in Drosophila.AD57, as a polypharmacological cancer therapeutic, is designed to regulate multiple targets related to cancer.

AD57 (hydrochloride)

C3080-10 10 mg
EUR 714
Description: IC50: 2 nM: blocks the receptor tyrosine kinase RET in Drosophila.AD57, as a polypharmacological cancer therapeutic, is designed to regulate multiple targets related to cancer.

AD57 (hydrochloride)

C3080-5 5 mg
EUR 428.4
Description: IC50: 2 nM: blocks the receptor tyrosine kinase RET in Drosophila.AD57, as a polypharmacological cancer therapeutic, is designed to regulate multiple targets related to cancer.

EDAC, hydrochloride

EB0433 25g
EUR 123.68

TAME Hydrochloride

GM4942-5G 5 g
EUR 90

TRIS hydrochloride

GB4431-100G 100 g
EUR 64.8

TRIS hydrochloride

GB4431-1KG 1 kg
EUR 160.8

TRIS hydrochloride

GB4431-250G 250 g
EUR 76.8

TRIS hydrochloride

GB4431-500G 500 g
EUR 103.2

TRIS hydrochloride

GB4431-5KG 5 kg
EUR 486

ACHP (Hydrochloride)

HY-13060 100mg
EUR 1861.2

MTEP (hydrochloride)

HY-13206 10mg
EUR 200.4

TAME hydrochloride

HY-13255A 100mg
EUR 192

TCEP (hydrochloride)

HY-W011500 10mM/1mL
EUR 135.6

MCLA hydrochloride

HY-W013275 5mg
EUR 381.6

DMCM (hydrochloride)

HY-100369A 100mg
EUR 1695.6

OABK (hydrochloride)

HY-100825 100mg
EUR 3531.6

EIPA (hydrochloride)

HY-101840A 10mM/1mL
EUR 151.2

(±)-ANAP hydrochloride

HY-101937C 5mg
EUR 817.2

EHNA (hydrochloride)

HY-103160A 5mg
EUR 170.4

PJ34 (hydrochloride)

HY-13688 10mg
EUR 159.6

MPEP (Hydrochloride)

HY-14609 50mg
EUR 439.2

S107 hydrochloride

HY-15292A 50mg
EUR 804

Inhibitory Potential of Polyclonal Camel Antibodies towards New Delhi Metallo-β-lactamase-1 (NDM-1)

New Delhi Metallo-β-lactamase-1 (NDM-1) is probably the most prevalent kind of metallo-β-lactamase, in a position to hydrolyze virtually all antibiotics of the β-lactam group, resulting in multidrug-resistant micro organism. Up to now, there aren’t any clinically related inhibitors to combat NDM-1. Using dromedary polyclonal antibody inhibitors towards NDM-1 represents a promising new class of molecules with inhibitory exercise.

Within the present research, immunoreactivities of dromedary Immunoglobulin G (IgG) isotypes containing heavy-chain and standard antibodies had been examined after profitable immunization of dromedary utilizing growing quantities of the recombinant NDM-1 enzyme. Inhibition kinetic assays, carried out utilizing a spectrophotometric methodology with nitrocefin as a reporter substrate, demonstrated that IgG1, IgG2, and IgG3 had been in a position to inhibit not solely the hydrolytic exercise of NDM-1 but in addition Verona integron-encoded metallo-β-lactamase (VIM-1) (subclass B1) and L1 metallo-β-lactamase (L1) (subclass B3) with inhibitory focus (IC50) values starting from 100 to 0.04 μM.

Investigations on the flexibility of IgG subclasses to cut back the expansion of recombinant Escherichia coli BL21(DE3)/codon plus cells containing the recombinant plasmid expressing NDM-1, L1, or VIM-1 confirmed that the addition of IgGs (four and eight mg/L) to the cell tradition was unable to revive the susceptibility of carbapenems.

Apparently, IgGs had been in a position to work together with NDM-1, L1, and VIM-1 when examined on the periplasm extract of every cultured pressure. The inhibitory focus was within the micromolar vary for all β-lactams examined. A visualization of the 3D structural foundation utilizing the three enzyme Protein Knowledge Financial institution (PDB) recordsdata helps preliminarily the recorded inhibition of the three MBLs.

A Easy and Environment friendly Genetic Immunization Protocol for the Manufacturing of Extremely Particular Polyclonal and Monoclonal Antibodies towards the Native Type of Mammalian Proteins

 

We’ve got generated polyclonal and monoclonal antibodies by genetic immunization over the past twenty years. On this paper, we current our most profitable methodology acquired over these years and current the animals by which we obtained the very best charges of success. The method introduced is handy, simple, inexpensive, and generates antibodies towards mammalian proteins of their native type.

This protocol requires neither costly gear, resembling a gene gun, nor subtle methods such because the conjugation of gold microspheres, electroporation, or surgical procedure to inject in lymph nodes. The protocol introduced makes use of merely the purified plasmid expressing the protein of curiosity below a powerful promoter, which is injected at intramuscular and intradermal websites.

This method was examined in 5 species. Guinea pigs had been the animals of alternative for the manufacturing of polyclonal antibodies. Monoclonal antibodies could possibly be generated in mice by giving, as a final injection, a suspension of transfected cells. The antibodies detected their antigens of their native kinds. They had been extremely particular with very low non-specific background ranges, as assessed by immune-blots, immunocytochemistry, immunohistochemistry and circulation cytometry. We current herein an in depth and easy process to efficiently elevate particular antibodies towards native proteins.

Manufacturing of a polyclonal antibody towards inosine-uridine preferring nucleoside hydrolase of Acanthamoeba castellanii and its entry to prognosis of Acanthamoeba keratitis

 

Acanthamoeba keratitis (AK) is a uncommon illness however its prevalence all through the globe continues to develop, primarily as a result of elevated contact lens utilization. Since early-stage signs related to AK carefully resemble these from different corneal infections, correct prognosis is tough and this typically leads to delayed therapy and exacerbation of the illness, which might result in everlasting visible impairment. Accordingly, creating a speedy Acanthamoeba-specific diagnostic methodology is very desired.

Within the current research, a speedy and . Among the many huge portions of proteins secreted by the pathogenic Acanthamoeba, an open studying body of the inosine-uridine preferring nucleoside hyddifferential methodology for AK prognosis was developed utilizing the secretory proteins derived from the pathogenic Acanthamoebarolase (IPNH) gene was obtained. After expressing and purifying the IPNH protein utilizing the pGEX 4T-Three vector system, mice had been immunized with the purified proteins for polyclonal antibody technology.

Western blot was carried out utilizing protein lysates of the human corneal cell, non-pathogenic amoeba, pathogenic amoeba, and scientific amoeba isolate together with lysates from different causes of keratitis resembling Staphylococcus aureus, Pseudomonas aeruginosa, and Fusarium solani to verify Acanthamoeba-specificity.

Western blot utilizing the polyclonal IPNH antibody revealed that IPNH was Acanthamoeba-specific since these proteins had been solely noticed in lysates of Acanthamoeba origin or its tradition media. Our findings point out that the IPNH antibody of Acanthamoeba might function a possible agent for speedy and differential AK prognosis.

RIPA Lysis Buffer (Strong)

HY-K1001 100 mL
EUR 176.4

Pierce Seal Strong Sheets

PCR0632 PK100
EUR 124.8

Pierce Seal Strong Roll610mX78mm

PCR0628 1ROLL
EUR 981.6

PCR Foil Seal Strong Sheets

PCR0528 PK100
EUR 188.4

Tweezers Fine Strong CF Tips

INS5058 EACH
EUR 33.42

Tweezers Flat Strong CF Tips

INS5100 EACH
EUR 39.04

Pierce Seal Strong Sample Roll

PCR0630 1ROLL
EUR 53.01

replacement tip Kit strong thick

INS2090 EACH
EUR 10.35

replacement tip Kit strong point

INS2094 EACH
EUR 10.35

replacement tip Kit strong thick

INS2058 EACH
EUR 10.35

EP Reagent Sodium Hydroxide Sol. Strong

1081404 1L
EUR 170.4

EP Reagent Sodium Hypochlorite Sol. Strong

10816005 500ML
EUR 121.2

Magnetic Base Support Z w/ Strong Magnet - 34.5mm

M-R-1013161 1 UNIT
EUR 46
Description: Magnetic Base Support Z w/ Strong Magnet - 34.5mm

Magnetic Base Support Z w/ Strong Magnet - 27.5mm

M-R-1015229 1 UNIT
EUR 46
Description: Magnetic Base Support Z w/ Strong Magnet - 27.5mm

Genistin

1535-10 each
EUR 196.8

Genistin

N1860-20 20 mg
EUR 268.8
Description: Actions Inactive analog of genistein; useful as a negative control for genistein and other tyrosine kinase inhibitors. Selective inhibitor of mammalian terminal deoxynucleotidyl transferase (TdT),with no measurable effect on mammalian or microbial DNA p

Genistin

HY-N0595 50mg
EUR 170.4

Genistin

TB0370-1000 4X20mg
EUR 321.6

Genistin

GC2802-10 10
EUR 242.4

Genistin

GC2802-100 100
EUR 960.1

Genistin

GC2802-25 25
EUR 382.3

Genistin

GC2802-250 250
EUR 1845.7

Genistin

GC2802-50 50
EUR 577.8

Genistin, >99%

BC034-025 25mg
EUR 278.4

Genistin, >99%

BC034-050 50mg
EUR 327.6

Genistin, >99%

BC034-100 100mg
EUR 537.6

Genistin(Genistoside)

E1KS2301 25mg
EUR 390

Genistin malonate

TB0588-0005 10mg
EUR 886.8

Optically clear sealing film for qPCR, Strong Bond, for PP plates, 100/pk

MS1000-PCR2 1 each
EUR 145

Foil seals, strong bonding for cold storage (-200 to 110°C) and DMSO, for PP plates, 100/pk

MS1000-F2 1 each
EUR 145

Strongzyme Goat anti Mouse IgG (H+L) (HRP)

43R-1653 1 ml
EUR 763.2
Description: Strongzyme Goat anti-Mouse IgG (H+L) secondary antibody (HRP)

Strongzyme Rabbit anti Goat IgG (H+L) (HRP)

43R-1650 1 ml
EUR 940.8
Description: Strongzyme Rabbit anti Goat IgG (H + L) (HRP) secondary antibody

Strongzyme Goat anti Rabbit IgG (H + L) (HRP)

43R-1652 1 ml
EUR 763.2
Description: Strongzyme Goat anti Rabbit IgG (H + L) secondary antibody (HRP)

QPCR Kit DNA Strongylus vulgaris

MOL8940 EACH
EUR 1004.01

QPCR Kit DNA Strongylus vulgaris

MOL8942 EACH
EUR 1274.27

Strongyloides (IgG/IgM) ELISA Kit (Human) (OKNA00174)

OKNA00174 96 Wells
EUR 505.2
Description: Description of target: Strongyloides is a genus containing some 50 species of obligate gastrointestinal parasites of vertebrates. Strongyloides stercoralis is the scientific name of a human parasitic roundworm causing the disease of strongyloidiasis. Its common name is pinworm in the UK and threadworm in the US. The Strongyloides stercoralis nematode can parasitize humans. The adult parasitic stage lives in tunnels in the mucosa of the small intestine.S. stercoralis can be found in areas with tropical and subtropical climates but cases also occur in temperate area, more frequently in rural areas. S. stercoralis has a very low prevalence in societies where fecal contamination of soil or water is rare. Many people infected are usually asymptomatic at first. Symptoms include dermatitis: swelling, itching, larva currens, and mild hemorrhage at the site where the skin has been penetrated. If the parasite reaches the lungs, the chest may feel as if it is burning, and wheezing and coughing may result, along with pneumonia-like symptoms (Löffler's syndrome). The intestines could eventually be invaded, leading to burning pain, tissue damage, sepsis, and ulcers. In severe cases, edema may result in obstruction of the intestinal tract, as well as loss of peristaltic contractions. Strongyloides infection in immunocompromised individuals (particularly following the administration of steroids, for example following transplant surgery) can result in disseminated strongyloidiasis, in which worms move beyond the confines of the gut into other organs. This is fatal unless antiStrongyloides therapy is given.Locating juvenile larvae, either rhabditiform or filariform, in recent stool samples will confirm the presence of this parasite. Other techniques used include direct fecal smears, culturing fecal samples on agar plates, serodiagnosis through ELISA, and duodenal fumigation.;Species reactivity: Human;Application: ;Assay info: Assay Methodology: Quantitative Reverse Capture Sandwich ELISA ;Sensitivity: Sensitivity is determined as the probability of the assay indicating a positive score in samples with the specific analyte present: 87.9%

StrongZyme Streptavidin-PolyHRP (High Load)

43R-1645 1 mg
EUR 369.6
Description: StrongZyme Streptavidin-PolyHRP conjugate (High Load)

Strontium Oxide

abx186564-500g 500 g
EUR 309.6

Strontium oxide, 99.5%

GX8751-50 50
EUR 585.6

ICP Std Strontium 1000ug/mL in 2-5% HCl

PSR2A3 100ML
EUR 120.12

ICP Std Strontium 1000ug/mL in 2-5% HCl

PSR2B3 250ML
EUR 214.12

ICP Std Strontium 1000ug/mL in 2-5% HCl

PSR2C3 500ML
EUR 265.04

ICP Std Strontium 10000ug/mL in 2-5% HCl

PSR4A3 100ML
EUR 208.9

ICP Std Strontium 10000ug/mL in 2-5% HCl

PSR4B3 250ML
EUR 352.51

Strontium bromide, 99.99%

GX4255-10 10
EUR 325.9

Strontium nitrate, 99.999%

GX6363-50 50
EUR 391

Strontium nitrate, 99.99%

GX1574-50 50
EUR 280.7

Strontium Ranelate

A8526-10 10 mg
EUR 397.2
Description: Strontium Ranelate is a bone metabolism modulator that inhibits bone resorption while maintaining bone formation. Commonly used as an antiosteoporotic.

Strontium chloride

B7708-50 50 mg
EUR 154.8

Strontium Ranelate

HY-17397 500mg
EUR 820.8

ICP Std Strontium 100ug/mL in 2-5% HNO3

PSR1A2 100ML
EUR 117.5

ICP Std Strontium 1000ug/mL in 2-5% HNO3

PSR2B2 250ML
EUR 214.12

ICP Std Strontium 1000ug/mL in 2-5% HNO3

PSR2C2 500ML
EUR 261.6

ICP Std Strontium 10000ug/mL in 2-5% HNO3

PSR4A2 100ML
EUR 208.9

ICP Std Strontium 10000ug/mL in 2-5% HNO3

PSR4B2 250ML
EUR 355.12

ICP Std Strontium 10000ug/mL in 3.5% HNO3

PSR4B4-500ML 500ML
EUR 671.08

Strontium fluoride, 99%

GX3818-250 250
EUR 137.2

Strontium titanate, 99.9%

GX4862-100 100
EUR 340.5

Strontium titanate, 99.9%

GX4862-25 25
EUR 164.5

Strontium fluoride, 99.99%

GX7221-10 10
EUR 134.1

Strontium fluoride, 99.99%

GX7221-25 25
EUR 286.8

Strontium fluoride, 99.99%

GX7221-50 50
EUR 562.1

Strontium fluoride, 99.9%

GX8262-10 10
EUR 116.1

Strontium fluoride, 99.9%

GX8262-50 50
EUR 280.2

Strontium titanate, 99+%

GX1727-250 250
EUR 108.5

AAS Strontium Std 1000ppm in 0.5M HNO3

AASRH 500ML
EUR 246

AAS Strontium Std 10000ppm in 1M HNO3

AASRM 500ML
EUR 356.4

Flame Std Strontium 1000ppm

FISR1 500ML
EUR 190.8

Strontium aluminate, 99+%

GX5543-10 10
EUR 113.1

Strontium aluminate, 99+%

GX5543-50 50
EUR 262.3

Strontium carbonate, 99.999%

GX5614-25 25
EUR 258.5

Strontium zirconate, 99+%

GX8495-250 250
EUR 120.5

Strontium molybdate, 99.9%

GX0022-50 50
EUR 189

Strontium tungstate, 99.9%

GX0472-10 10
EUR 101

Strontium tungstate, 99.9%

GX0472-50 50
EUR 233

Strontium carbonate

GK8492-1 1
EUR 50.3

Strontium carbonate

GK8492-250 250
EUR 25.5

Strontium carbonate

GK8492-5 5
EUR 176.1

Strontium carbonate, 99+%

GX9308-100 100
EUR 129.6

Strontium carbonate, 99+%

GX9308-250 250
EUR 229.6

IC Cation Std Strontium 200ppm in H2O

ICCT43 100ML
EUR 135.6

ICP Standard Strontium 2-5pc

PSR2A2 100ML
EUR 221.95

Strontium Pieces max. 8 mm, under oil, 99.2+%

GX0495-25 25
EUR 1023.2

IC Cation Std Strontium 1000ppm in 0.005% HNO3

ICCB43 500ML
EUR 406.8

IC Cation Std Strontium 1000ppm in 0.005% HNO3

ICCS43 100ML
EUR 157.98

IC Cation Std Strontium 100ppm in 0.005% HNO3

ICCU43 100ML
EUR 137.09

Strontium acetate hydrate, 99+%

GX5175-100 100
EUR 126.5

Strontium chloride hydrate, 99.99+%

GX6973-10 10
EUR 172.7

Strontium nitrate, anhydrous, 98%

GK5693-1KG 1 kg
EUR 98.4

Strontium nitrate, anhydrous, 98%

GK5693-250G 250 g
EUR 60

Strontium nitrate, anhydrous, 98%

GK5693-5KG 5 kg
EUR 255.6

Strontium nitrate, anhydrous, 98%

GK5693-1 1
EUR 51.4

Strontium nitrate, anhydrous, 98%

GK5693-250 250
EUR 19.8

Strontium nitrate, anhydrous, 98%

GK5693-5 5
EUR 182

Strontium titanate Nanopowder, 99.9%

GX7135-1 1
EUR 633.8

Strontium titanate Nanopowder, 99.9%

GX7135-100 100
EUR 192.2

Strontium titanate Nanopowder, 99.9%

GX7135-250 250
EUR 281.9

Strontium titanate Nanopowder, 99.9%

GX7135-50 50
EUR 116.1

Strontium titanate-Nano Powder, 99.8%

GX8434-100 100
EUR 192.2

Strontium titanate-Nano Powder, 99.8%

GX8434-250 250
EUR 373

Strontium titanate-Nano Powder, 99.8%

GX8434-50 50
EUR 116.1

Strontium chloride hexahydrate

GK3876-1KG 1 kg
EUR 132

Strontium chloride hexahydrate

GK3876-500G 500 g
EUR 91.2

Strontium chloride hexahydrate

GK3876-5KG 5 kg
EUR 370.8

Strontium chloride hexahydrate

GK3876-1 1
EUR 79.1

Strontium chloride hexahydrate

GK3876-5 5
EUR 276.9

Strontium chloride hexahydrate

GK3876-500 500
EUR 45.9

Genistein

1533-10 each
EUR 164.4

Genistein

1533-100 each
EUR 326.4

Genistein

A2198-100 100 mg
EUR 129.6
Description: Phytoestrogen with a wide range of biological actions. Inhibits protein tyrosine kinases including epidermal growth factor receptor kinase. Also binds to PPAR? and estrogen receptors and acts as an agonist at GPR30. Also available as part of the MAP

Genistein

A2198-5.1 10 mM (in 1mL DMSO)
EUR 135.6
Description: Phytoestrogen with a wide range of biological actions. Inhibits protein tyrosine kinases including epidermal growth factor receptor kinase. Also binds to PPAR? and estrogen receptors and acts as an agonist at GPR30. Also available as part of the MAP

Genistein

20-abx076504
  • EUR 260.40
  • EUR 360.00
  • 10 mg
  • 50 mg

Genistein, >99%

BC033-101 1g
EUR 278.4

Genistein, >99%

BC033-105 5g
EUR 457.2

Genistein, >99%

BC033-110 10g
EUR 813.6

Genistein

20-abx184098
  • EUR 326.40
  • EUR 1378.80
  • EUR 594.00
  • 1 g
  • 25 g
  • 5 g

Genistein

GP7710-100MG 100 mg
EUR 180

Genistein

GP7710-10MG 10 mg
EUR 92.4

Genistein

GP7710-250MG 250 mg
EUR 237.6

Genistein

GP7710-50MG 50 mg
EUR 154.8

Genistein

GP7710-5MG 5 mg
EUR 74.4

Genistein

HY-14596 100mg
EUR 142.8

Genistein

TB0320-0500 8XX25mg
EUR 328.8

Genistein

GP7710-10 10
EUR 74.7

Genistein

GP7710-100 100
EUR 118.7

Genistein

GP7710-250 250
EUR 180.9

Genistein

GP7710-5 5
EUR 31.7

Genistein

GP7710-50 50
EUR 116.1

Strontium ranelate heptahydrate

20-abx184380
  • EUR 444.00
  • EUR 944.40
  • 1 g
  • 500 g

Strontium hydroxide, octahydrate, 99+%

GX1288-1 1
EUR 101

Strontium hydroxide, octahydrate, 99+%

GX1288-500 500
EUR 84.4

Assurance Grade Strontium for AA and ICP 1000 ug/mL (1000 PPM) 500mL in 2% HCl

PLSR1-2X 500ML
EUR 158.4

Genistein 10mM

TG4160 0.25ml
EUR 187.2

Assurance Grade Strontium for AA and ICP 1000 ug/mL (1000 PPM) 30mL in 2% HNO3

PLSR2-2M 30ML
EUR 34.8

Assurance Grade Strontium for AA and ICP 1000 ug/mL (1000 PPM) 250mL in 2% HNO3

PLSR2-2T 250ML
EUR 111.6

Assurance Grade Strontium for AA and ICP 1000 ug/mL (1000 PPM) 500mL in 2% HNO3

PLSR2-2X 500ML
EUR 144

Assurance Grade Strontium for AA and ICP 1000 ug/mL (1000 PPM) 125mL in 2% HNO3

PLSR2-2Y01 125ML
EUR 64.8

Assurance Grade Strontium for AA and ICP 10000 ug/mL (10000 PPM) 500mL in 5% HNO3

PLSR2-3X 500ML
EUR 255.6

Assurance Grade Strontium for AA and ICP 10000 ug/mL (10000 PPM) 125mL in 5% HNO3

PLSR2-3Y 125ML
EUR 108

Strontium Chloride Diluting Solution

SRCL201 1L
EUR 72

Kinesis Hollow Cathode Lamp Strontium 37mm Std

CHR6011 EACH
EUR 334.23

Claritas PPT Grade Strontium for ICP-MS 1 ug/mL (1 PPM) 125mL in 2% HNO3

CLSR2-1BY 125ML
EUR 103.2

Strontium chloride hexahydrate, 99%, ACS grade

GX3549-500G 500 g
EUR 122.4

Strontium Pieces max. 20 mm, under argon, coated with oil, Ba max. 1%, 99%

GX0588-100 100
EUR 550.7

Strontium chloride hexahydrate, 99%, ACS grade

GX3549-500 500
EUR 71.1

Kinesis Hollow Cathode Lamp Strontium 37mm unicam

CHR6015 EACH
EUR 385.15

Kinesis Hollow Cathode Lamp Strontium 37mm Varian

CHR6017 EACH
EUR 385.15

Genistein 8-c-glucoside

HY-N6882 10mM/1mL
EUR 1182

Kinesis Hollow Cathode Lamp Strontium 50mm Standard

CHR6019 EACH
EUR 412.57

Kinesis Hollow Cathode Lamp Strontium 50mm PE AAnalyst (Lumina)

CHR6021 EACH
EUR 539.21

Genistein (Tyrosine kinase inhibitor)

SIH-446-10MG 10 mg
EUR 117.6
Description: The substance Genistein is a tyrosine kinase inhibitor. It is synthetically produced and has a purity of ?98%. The pure substance is creamy off-white solid which is soluble in DMSO (30 mg/ml), 100% ethanol (4 mg/ml) or dilute aqueous base; insoluble in water.

Genistein (Tyrosine kinase inhibitor)

SIH-446-50MG 50 mg
EUR 198
Description: The substance Genistein is a tyrosine kinase inhibitor. It is synthetically produced and has a purity of ?98%. The pure substance is creamy off-white solid which is soluble in DMSO (30 mg/ml), 100% ethanol (4 mg/ml) or dilute aqueous base; insoluble in water.

Genistein 7,4'-di-O-β-D-glucopyranoside

TBW00747 10mg
EUR 631.2

Genistein 7-O-β-D-glucopyranoside-4'-O-[α-L-rhamnopyranosyl-(1→2)-β-D-glucopyranoside]

TBW00759 10mg
EUR 886.8

4''-methyloxy-Genistin

HY-N4129 5mg
EUR 531.6

4''-methyloxy-Genistin

TBZ2680 5mg Ask for price

Anti-Hu STRO-1 PE

1P-649-C025 0.025 mg
EUR 168

Anti-Hu STRO-1 PE

1P-649-C100 0.1 mg
EUR 288

Anti-Hu STRO-1 APC

1A-649-C025 0.025 mg
EUR 168

Anti-Hu STRO-1 APC

1A-649-C100 0.1 mg
EUR 288

STRO-1 Antibody

abx140247-01mg 0.1 mg
EUR 610.8

STRO-1 Antibody (PE)

abx140285-01mg 0.1 mg
EUR 577.2

STRO-1 Antibody (APC)

abx140284-01mg 0.1 mg
EUR 577.2

Anti-Hu STRO-1 Purified

11-649-C025 0.025 mg
EUR 178.8

Anti-Hu STRO-1 Purified

11-649-C100 0.1 mg
EUR 309.6

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