Evaluation of Laboratory Application of Camelid Sera Containing Heavy-Chain Polyclonal Antibody

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Evaluation of Laboratory Application of Camelid Sera Containing Heavy-Chain Polyclonal Antibody Against Recombinant CTLA-4 Protein. Cytotoxic T-lymphocyte-associated protein-4 (CTLA-4) is a critical negative immunomodulatory receptor that is normally expressed in activated T cells and noticeably, in many cancerous cells. Indeed, molecular detection of CTLA-4 protein is crucial in basic research. In this work, the extracellular domain of the […]

Efficient detection of eukaryotic calcium-sensing receptor

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In direction of middle-up evaluation of polyclonal antibodies: subclass-specific N-glycosylation profiling of murine immunoglobulin G (IgG) by the use of HPLC-MS   Lately, superior HPLC-MS methods based mostly on intact protein (“top-down”) or protein subunit (“middle-up/middle-down”) evaluation have been applied for the characterization of therapeutic monoclonal antibodies. Right here, we assess feasibility of middle-up/middle-down evaluation for polyclonal […]

In utero exposure to endogenous maternal polyclonal anti-Caspr2 antibody

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Preparation and Characterization of Two Immunogens and Manufacturing of Polyclonal Antibody with Excessive Affinity and Specificity for Darunavir Darunavir (DRV) is a potent antiviral drug used for therapy of infections with human immunodeficiency virus (HIV). Efficient and protected therapy with DRV requires its therapeutic drug monitoring (TDM) in affected person’s plasma throughout remedy. To assist TDM of DRV, […]

Prokaryotic expression of PE8 protein from Mycobacterium tuberculosis

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Improvement of a Extremely Delicate Enzyme-Linked Immunosorbent Assay for Mouse Soluble Epoxide Hydrolase Detection by Combining a Polyclonal Seize Antibody with a Nanobody Tracer   Enzyme-linked immunosorbent assays (ELISA) for the detection of soluble epoxide hydrolase (sEH), a key enzyme within the metabolism of fatty acids and a biomarker, might more and more signify an essential diagnostic software. Nonetheless, […]

Expression of HPV16 E6 recombinant protein and preparation of its rabbit polyclonal antibody

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Era of Excessive Affinity Anti-Peptide Polyclonal Antibodies Recognizing Goat α s1-Casein   The chemical, technological and allergy properties of goat’s milk are considerably affected by the extent of αs1-casein. Detection and quantification of αs1-casein requires high-specificity strategies to beat high-sequence similarity between this protein and others within the casein household. Unavailability of antibodies with excessive affinity and specificity in […]

Preparation of a highly specific polyclonal antibody against and its use in development of ELISA

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The prokaryotic expression, polyclonal antibody preparation, and subcellular localization of the transmembrane protein NS2A of the duck Tembusu virus   Duck Tembusu virus (DTMUV) is a single-stranded, positive-sense RNA arbovirus, belonging to the genus Flavivirus, household Flaviviridae. As a transmembrane protein, non-structural protein 2A (NS2A) performs an vital position in virion meeting, replication advanced and antagonizing host […]